Plate staining

Cells were washed, and half were treated with neuraminidase (see Methods). Other half were treated identically in the absence of enzyme. Lipid extracts later made from neuraminidase-treated and control samples in the usual way, and extracts from 6 X Iff1 cells were compared by TLC. Plates stained with a-naphthol/sulfuric acid blue areas are bracketed. ( —) Controls (+) treated with neuraminidase. 

FICURE19.1 Pictures of the chromatograms on the cellulose plates stained with ninhydrin and valid for (a) DL-proline and (b) L-proline (1) proline-derived oligopeptide fraction (2) L-proline and (3) D-proline. Samples were applied to the plate in the aliqnots of 5 pL, at the concentration of 1.0 mg mL amino acid (plus equimolar amount of Mn(II) acetate) and developed with 2-butanol -I- pyridine -I- glacial acetic acid -I- water (30 20 6 24, v/v). (From Sajewicz, M. et al. 2013. J. Liq. Chromatogr. Relat. Technol., 36 2497. With permission.)... 

Fig. 4 Mortierella alpina (a) asexual lifecycle of the fungus. Haploid cells form sporangiophores, and sporangiospores germinate to hypha. (b) Fungal culture grown rai PDA plate stained with 0.5 % triphenyl tetrazolium chloride. Lipid droplets are stained brown (Wang et al. 2011)...

Water Tests. In colorfastness to water, ISO 10S-E01, the test specimen is placed in contact with the chosen adjacent fabrics, immersed in water, and placed wet between glass plates and left for 4 h at 37°C. After drying, the effect on the test specimen and stain on adjacents are assessed. The test, colorfastness to seawater, ISO 10S-E02, is the same as EOl but uses 30 g/L anhydrous sodium chloride solution instead of water. To test for colorfastness to chlorinated seawater/swimming baths water, ISO 10S-E03, the specimen is immersed in sodium hypochlorite solution containing either 100, 50, or 20 mg of active chlorine per Hter at pH 7.5 for 1 h at 27°C, rinsed, dried, and assessed. 

Poor preparation of the substrate can result in loss of adhesion, pitting, roughness, lower corrosion resistance, smears, and stains. Because electroplating takes place at the exact molecular surface of a work, it is important that the substrate surface be absolutely clean and receptive to the plating. In the effort to get the substrate into this condition, several separate steps may be required, and it is in these cleaning steps that most of the problems associated with plating arise. 

Tin—Nickel. AHoy deposits having 65% fin have been commercially plated siace about 1951 (135). The 65% fin alloy exhibits good resistance to chemical attack, staining, and atmospheric corrosion, especially when plated copper or bron2e undercoats are used. This alloy has a low coefficient of friction. Deposits are solderable, hard (650—710 HV ), act as etch resists, and find use ia pfinted circuit boards, watch parts, and as a substitute for chromium ia some apphcafions. The rose-pink color of 65% fin is attractive. In marine exposure, tin—nickel is about equal to nickel—chromium deposits, but has been found to be superior ia some iadustfial exposure sites. Chromium topcoats iacrease the protection further. Tia-nickel deposits are bfitde and difficult to strip from steel. Temperature of deposits should be kept below 300°C. 

The progress of the reaction may be followed by analytical thin-layer chromatography on alumina. The submitters used polygram pre-coated plastic sheets (Alox N/UV254) purchased from Macherey-Nagel, Inc. The plates were developed with 1 1 hexane-ether and stained with basic permanganate. The Rf of the product is 0.56. 

Do not Handle damaged, leaking or stained packages Load a freight container without valid safety approval plate... 

Aqueous solutions of dyes ean also be employed instead of water. In the ease of hydrophilic dyes such as methylene blue or patent fast blue the transparent background of the TLC/HPTLC plate is stained blue. Pale spots occur where there are nonwetted zones. Dauble [89] detected anion-active detergents in this way on silica gel layers as pale zones on a blue background with palatine fast blue GGN... 

Figure 5. Citrate agar electrophoresis of hemoglobins. The plate is stained with O-dimethyb...

Figure 41, The principle of the tape system of analysis. The three tapes are the reagent tape on the bottom, cellopnarie in the center, and sample tape on top. The sample placed on the top tape folds over and is pressed by the press plate and warmed so that the material dialyses through the membrane. The stain is then formed and is read by reflecting densitometry on the reagent tape.

Total pectinase, cellulase and lipase activities secreted by colonies were detected on BSM plates containing respectively 1% of citrus pectin, 2% Walseth cellulose and 1% olive oil + rhodamine. After few days at 30°C, pectin plates were covered by 1% CTAB for Ihour, positive colonies became surrounded by a clear halo walseth plates are not stained the halo is visible directly on positive clones lipase activity is revealed under UV on oil-rhodamine plates. 

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