Fast blue

Depending upon the metallic compound used, different metallic phthalocyanine derivatives are obtained, e.g. when copper chloride is used copper phthalocyanine (Monastral Fast Blue B) is obtained. 

Dyes polyester fibers fast blue, brown, and red shades... 

Aqueous solutions of dyes ean also be employed instead of water. In the ease of hydrophilic dyes such as methylene blue or patent fast blue the transparent background of the TLC/HPTLC plate is stained blue. Pale spots occur where there are nonwetted zones. Dauble [89] detected anion-active detergents in this way on silica gel layers as pale zones on a blue background with palatine fast blue GGN... 

Dipping solution Dissolve 140 mg fast blue salt B in 10 ml water and make up to 40 ml with methanol. Add 20 ml of this solution with stirring to a mixture of 55 ml methanol and 25 ml dichloromethane. 

Fast Blue Salt B Reagent 289 Spray solution II Caustic soda solution (c = 0.1 mol/1)... 

Fast blue salt B couples best with phenols in alkaline medium, e.g. with 11-nor-d -THC-9-carboxylic acid to yield the following red-colored product ... 

Note The detection is not affected if the dipping solution exhibits a slight opalescent turbidity. Fast blue salt BB [18] or fast blue salt RR [18,19] can be employed in the reagent in place of fast blue salt B. It is occasionally preferable not to apply spray solutions I and II separately but to work directly with a 0.1% solution of fast blue salt B in caustic soda solution (c=l—2mol/l) [13, 15] or in 0.5% methanolic caustic potash [3]. 

A dipping solution consisting of 0.2% fast blue salt B in hydrochloric acid (c = 0.5 mol/1, immersion time 30 s) has been reported for the detection of resorcinol homologues [1]. 

Fig. 1 Chromatograms of urine samples containing THC metabolites detection with fast blue salt RR (A) and fast blue salt B (B). Track Ai and B, metabolite-free urines tracks A,6 and B15 represent ca. 60 ng total cannabinoids per ml urine (determined by RIA).

Note The alternative fast blue salt BB produced the most intensely colored chromatogram zones for visual analysis in daylight, while fluorescence quenching in UV light (A = 254 nm) was greater with fast blue salt B and fast blue salt RR (Figs. 1 and 2). 

Bcht-base,/. (Dyeing) fast color base, -baum-wollblau, n. fast cotton blue, -blau, n. fast blue, -braun, n. fast brown, -farben, n. fast dyeing. 

Fast blue salt B, fast blue salt BB, fast black salt K, diazotized sulfanilic acid (Pauly s reagent), diazotized sulfanilamide or 4-nitroaniline... 

Phenols (capable of coupling) Fast blue salt B, fast blue salt BB, fast black salt K, diazotized sulfanilic acid (Pauly s reagent) diazotized sulfanilamide or 4-nitroaniline Intensely colored azo dyes are formed. Catecholamines [20, 3S], imidazoles [21] and amines capable of coupling also react. [3, 17]... 

It is also probable that there is coupling to colored derivatives in the case of aromatic amines and phenols (cf. Fast blue salt B reagent). 

Dipping solution Dissolve O.S g fast blue salt BB (C.I. 3717S) in 2S ml water and make up to 100 ml with methanol [1]. 

Spray solution Dissolve 0.5 g fast blue salt BB in 100 ml water [2]. 

Fast blue salt BB couples with phenols, preferably in alkaline medium, to yield intensely colored azo dyes. 

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