Walseth cellulose

Total pectinase, cellulase and lipase activities secreted by colonies were detected on BSM plates containing respectively 1% of citrus pectin, 2% Walseth cellulose and 1% olive oil + rhodamine. After few days at 30°C, pectin plates were covered by 1% CTAB for Ihour, positive colonies became surrounded by a clear halo walseth plates are not stained the halo is visible directly on positive clones lipase activity is revealed under UV on oil-rhodamine plates. 

The Cl enzymes, hydrocellulases, and Avicelases may now be correctly and more specifically termed cellobiohydrolases because of their action on cellooligosaccharides and Walseth cellulose. The Cx enzymes may now be classified as endo- or xo-glucanases, or /5-glucosidases. We therefore suggest abandoning the Ci-Cx terminology in favor of a more precise one based on the hydrolytic activities of the component enzymes. 

Degree of Crystallinity. The influence of degree of crystallinity on the susceptibility of cellulose to enzymatic hydrolysis has been studied by Norkrans (48), Walseth (74, 75) and Reese, Segal, and Tripp (57), among others. Using several cellulose samples precipitated after swelling in phosphoric acid, Walseth (74) showed that the preparations with... 

With crude enzymes from Aspergillm niger imder optimal conditions (for this enzyme, pH 4.5 at 47 C.), Walseth 60) was able to degrade a particularly reactive cellulose (preswollen with phosphoric acid) to the extent of 96 % and to account for the loss of weight as D-glucose (by reducing-sugar determinations). 

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