P-£>-glucuronidase

Erythrocyte-entrapped P-glucuronidase has been retained in the circulation and in the Hver of P-glucuronidase-deficient mice for longer periods of... 

Rabbit peritoneal neutrophils were harvested and their release of p-glucuronidase was measured at 37°C, as described previously (13). For indo-1, neutrophils were washed twice in a calcium-free buffer, then loaded with 15 indo-1 acetoxymethyl ester (24) for 40 min at 37 C at a density of 5 x 10 cells/ml. The cells were then washed twice more in calcium-free buffer, resuspended to a density of 1 X 10 cells/mL, and kept on ice. Prior to fluorometry, cells were diluted 4x with the appropriate buffer at 37 C. For CTC, neutrophils were incubated with 20 pH CTC at 37°C in the spectrofluorometer cuvette. All measurements were carried out using an SLH-Aminco SPF 500C fluorospectrometer interfaced with an IBM PC microcomputer. 

Figure 4. Effect of hyperosmolality on lysosomal enzyme release from rabbit neutrophils. Cells were preincubated 10 min at 37 C in either regular HEPES buffer at 320 mosmol/kg ( ) or in HEPES buffer with 0.3-M sucrose at 680 mosmol/kg ( ), 5 Mg/mL cyto-chalasin B was added, cells were stimulated with FMLP, and p-glucuronidase was released into the medium during a 6-min period measured.

Figure 5. Inhibition of lysosomal enzyme release from neutrophils by increased osmotic strength. Cells were preincubated for 10 min at 37°C in regular buffer containing no additions (o), or containing sodium sulfate ( ), sodium HEPES ( ), or sucrose ( ) to increase the osmotic strength. Cells were treated with cyto-chalasin B (5 arid FMLP (10" M) and p-glucuronidase was...

MIM 253200) Sly (MIM 253220) MPS VII sulfatase (arylsulfatase B) P-Glucuronidase Dermatan sulfate, heparan sulfate, chondroitin... 

The first proteins from transgenic plants to reach commercial status were avidin and P-glucuronidase (GUS) both of which are used as diagnostic agents in molecular biology. An important principle demonstrated by these case studies is that molecular farming in cereals can be an economical alternative even when the natural source of a protein is abundant (i.e. egg whites for avidin, and Escherichia coli for GUS) and where a market is already established. 

Two studies have involved the fusion of an antigen to P-glucuronidase (GUS). Gil et al. [61] fused GUS to the 2L21 protective epitope from canine parvovirus, and Dus Santos et al. [23] fused GUS to the protective epitope from FMDV. Transformants in both cases were selected on the basis of GUS activity, and both proved to be immunogenic. Mice immunized orally or parenterally with the GUS-FMDV epitope fusion were completely protected against challenge with the native virus. 

Sly syndrome MPS VII P-glucuronidase Heparan sulfate Dermatan sulfate... 

Transgenic tomato plants Wound induced vascular bundle-specific expression of P-glucuronidase gene Jacinto et al. (14)... 

Glueuronidase E. coli, A. aerogenes Cleavage of P-glucuronidases of alcohols and phenols... 

Amines may form N-glucuronides that, unlike 0-glucuronides, are resistant to bacterial p-glucuronidases. 

Administered in the drinking water to male rats for 60 days, 0.7 mg thallium/day, as thallium sulfate, caused abnormalities in testicular morphology, function, and biochemistry. Effects included increased epididymal sperm with increased numbers of immature cells, decreased sperm motility, and reduced testicular P-glucuronidase. 

Lampe, J.W., Li, S.S., Potter, J.D., and King, I.B., Serum p-glucuronidase activity is inversely associated with plant-food intakes in humans, J. Nutr., 132, 1341, 2002. 

Marshall, T., Shult, P., and Busse, W.W., Release of lysosomal enzyme p-glucuronidase from isolated human eosinophils, J. Allergy Clin. Immunol, 82, 550, 1988. 

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