P-D-glucuronidase

In 2007, Chou s group (2007) (112) conducted an investigation on the physical adsorption of anti-p-D-glucuronidase (GUD) antibodies onto the unclad region of the PMMA fiber core. This was done by incubating the fiber in the... 

Mucopolysaccharidosis VII (Sly syndrome) P-D-Glucuronidase HS, DS Short stature, skeletal dysplasia, coarse facial features, joint stiffness, visceromegaly, CNS involvement, cardiac disease... 

S-Carboxy-3/ ,4/ ,55 -trihydroxy-piperidine from Baphia racemosa (Leguminosae) seeds is an inhibitor of human P-D-glucuronidase and a-L-iduronidase [147]. The all trans-dihydroxyproline, (25, 3/ ,4f )-2-carboxy-3,4-dihydroxypyrrolidine, from Amanita virasa is structurally analogous at C(2), C(3) and C(4) to O-glucuronic acid and it is also a moderate inhibitor of human P-D-glucuronidase [148]. 

However, the absence of detection of phenotypically atypical STEC 0157 with media based on sorbitol-fermenting profde and P-D-glucuronidase activity still remains a problem. 

This is the reason why non-circulating human enzymes appeared us as better candidates for prodrug activation in the ADEPT approach. We selected in our work the human lysosomal glycosidases a-D-galactosidase and p-D-glucuronidase. An additional argument for the choice of these enzymes is that they can be used in conjunction with highly hydrophilic... 

The therapeutic efficacy of the combined use of the humanized anti-carcinoembryogenic mococlonal antibody-human P-D-glucuronidase fusion protein (48) and of the prodrug 73 (66) was demonstrated in nude mice... 

Sample preparation Prepare urine samples by adjusting pH to 5.0 with 2 M HCl then hydrol3 ing with 20 p,L 20 000 U/mL p-D-glucuronidase at 37° for 5 h. 500 pL Plasma or urine + 15 p,L 2.5 mg/L desmethylflunitrazepam in MeOH 50 pL 0.5 M NaOH + 5 mL diethyl ether, agitate, centrifuge, evaporate to dryness at 45° under vacuum, take up residue in 100 p,L MeOH, iiyect 25 pL aliquot. 

The clearance of P-D-2-acetamido-2-deoxyglucosidase from the circulatory system and the clearance induced by modified glycoproteins have been studied in rats. Intravenous infusion of a purified P-D-2-acetamido-2-deoxyglucosidase from rat epididymis showed that the enzyme is cleared comparatively slowly. Kinetic and competitive experiments with P-D-2-acetamido-2-deoxyglucosidase and P-D-glucuronidase indicated that the lysosomal compartment of rat liver has an important role in clearing the enzymes. ... 

The rate of removal of purified P-D-glucuronidases from rats following intravenous infusion depended on the source of the enzyme. Thus, the P-D-glucuronidase from rat serum was cleared more slowly from circulation than that from rat preputial glands. The lysosomal compartment of rat liver has an important role in the removal of circulating tris and glycoside hydrolases. ... 

The p-D-glucuronidase from the preputial glands of rats crystallized following fractional precipitation, fractionation in ethanol, and gel filtration. The purified enzyme (12S, mol. wt. 3.2 x 10 ) appeared to be homogeneous on examination by polyacrylamide gel electrophoresis, but electrophoresis in the presence... 

Injection of alkyl phosphates into rats raised the level of P-D-glucuronidase activity in their sera without affecting the levels of lysosomal hydrolases and cholinesterase. Dibutyl and tributyl phosphates were most and equally effective, the levels of P-D-glucuronidase activity increasing 120- and 90-fold after 1 and 2 h, respectively. The increase in enzymic activity elicited with tributyl phosphate correlated with a lowering of the enzymic activity in the liver microsomes, which appear to be the main source of the excess of P-D-glucuronidase activity in the serum. 

The removal of p-D-glucuronidase from the circulatory system of rats has been investigated. ... 

Anti- Phaseolus vulgaris a-o-mannosidase) antibody Anti- Phaseolus vulgaris a-D-mannosidase I) antibody immunoglobulin IgG Anti-(rat p-D-glucuronidase) antibody... 

Purification of rat-liver p-D-glucuronidase Sulphide-disulphide interchange chromatography in the separation of plasma proteins... 

P-D-Glucuronidases.—Removal of the terminal neuraminic acid residues from rabbit-liver /S-D-glucuronidase decreased the number of multiple forms, chiefly those of lowest p7, observed by isoelectric focusing. ... 

D.-M. He, I. Kaleem, S.-Y. Qin, D.-Z. Dai, G.-Y. Liu, C. Li, Biosynthesis of glycyrrhetic acid 3-O-mono-P-D-glucuronide catalyzed by p-D-glucuronidase with enhanced bond selectivity in an ionic liquid/buffer biphasic system. Process Biochem. 45 (2010) 1916-1922. 

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