Ornithine decarboxylase activity

Administration of single oral doses of 90 or 120 mg/kg mirex by gavage to female Sprague-Dawley rats resulted in induction of hepatic ornithine decarboxylase activity there was, however, no evidence of significant damage to deoxyribonucleic acid (DNA) as measured by alkaline elution (Mitra et al. 1990). 

In agreement with hepatic functional activity studies conducted with mirex, chlordecone administered orally to female Sprague-Dawley rats at 1/5 and 3/5 of the LDso (19 and 57 mg/kg, respectively) caused a significant increase in ornithine decarboxylase activity, but there was no evidence of DNA damage at either level (Kitchin and Brown 1989). 

In animal studies, mirex (a nonmutagenic hepatocarcinogen) promoted mouse skin squamous carcinomas and papillomas after initiation with 7,12-dimethyl-benz[a]anthracene (DMBA) for 1 week. Mirex, also, potentiated the promotional potency of the phorbol ester tumor promoter, 12-0 -tetradecanoylphorbol-13-acetate (TPA). There was a 90% incidence (activation) of the c-Ha-ras tumor gene in these co-promoted tumors. When both mirex and TPA gave a similar tumor yield, only the TPA response was associated with biochemical markers of enhanced cell proliferation, induction of epidermal ornithine decarboxylase activity and increased DNA synthesis, and hyperplasia. Thus, there is evidence for a dual effect of mirex during co-promotion first, as an independent tumor promoter with a mechanism different than that of phorbol esters and second, as a compound that also potentiates skin tumor promotion by TPA (Meyer et al. 1993, 1994 Moser et al. 1992, 1993). 

Yatin SM, Yatin M, Aulick T, Ain KB, Butterfield DA. (1999). Alzheimer s amyloid beta-peptide associated free radicals increase rat embryonic neuronal polyamine uptake and ornithine decarboxylase activity protective effect of vitamin E. Neurosci Lett. 263(1) 17-20. 

Savage RE Jr., Westrich C, Guion C, et al. 1982. Chloroform induction of ornithine decarboxylase activity in rats. Environ Health Perspect 46 157-162. 

Phillips, R. W., Kikendall, J. W., Luk, G. D., Willis, S. M., Murphy, J. R., Maydonovitch, C., Bowen, P. E., Stacewicz-Sapuntzakis, M., and Wong, R. K. (1993). Beta-carotene inhibits rectal mucosal ornithine decarboxylase activity in colon cancer patients. Cancer Res. 53, 3723-3725. 

Ornithine decarboxylase activity. Fixed oil (4.5%), Clupeidae brevortia tyrannus (4%), and Zea mays (1.5%) fixed oil (7.5%), Clupeidae brevortia tyrannus (1%), and Zea mays (1.5%) fixed oil (8.5%) and Zea mays (1.5%), administered orally to mice for 1 year, were active vs benzoyl peroxide-induced ornithine decarboxylase activity Oil, administered to 30 ultraviolet (UV)-irradiated Senear and SKH-1 mice at doses of 1/14% (A diet), 7.9/7.1% (B diet), and 15/0% (C diet) corn oil/coco-nut oil for 6 weeks, produced no increase in enzyme activity. The level of ornithine decarboxylase activity in the UV-irradiated mice fed diet A was significantly higher than in mice fed the B or C diet. In the SKH-1 mice, ornithine decarboxylase activity was increased by 3 weeks and was significantly higher in mice fed diet C than in mice fed diet A. There was no significant effect of dietary fat on UV-induced skin tumor incidence ". 

CN210 Berton, T. R., S. M. Fischer, C. J. Conti, and M. F. Locniskar. Comparison of ultraviolet light-induced skin carcinogenesis and ornithine decarboxylase activity in sencar and hairless SKH-1 mice fed a constant level of dietary lipid varying in com and coco-... 

Epstein-Barr virus early antigen induction. Methanol extract of the dried leaf, in cell culture at a concentration of 1 pg/mL, was inactive. The assay was designed for tumor-promoting activity . Two diastere-oisomers of 2,7,1 l-cembratriene-4,6-diol (a- and 3-CBT) from the neutral fractions of cigarette smoke condensate, in Raji cells, produced potent inhibitory effects on the induction of Epstein-Barr virus (EBV)-EA by 12-0-tetradecanoylphorbol-13-acetate (TPA). The doses of a- and P-CBT required for 50% inhibition of EBV-EA induction by TPA were 7.7 and 6.7 mg/mL, respectively. Application of a- and P-CBT to mouse skin before treatment with TPA, inhibited TPA-induced ornithine decarboxylase activity in a dose-dependent manner. Application of 16.5 pM/mouse of a- and p-CBT resulted... 

Ornithine decarboxylase activity. Cigarette smoke was administered to intact animals and animals with ulcers at concentrations of 2 or 4%. The treatment significantly reduced the thickness of the mucous secreting layer and gastric mucosal ornithine decarboxylase activity in animals with or without ulcers. The extract significantly reduced mucus synthesis and ornithine decarboxylase activity but not its mRNA expression in MKN-28 cells " . Cigarette smoke and its extract, in human MKN-28 cells, markedly decreased mucus synthesis in vivo and in vitro and suppressed ornithine decarboxylase activity . 

Wang, and C. H. Cho. Interactions of EGF and ornithine decarboxylase activity in the regulation of gastric mucus synthesis in cigarette smoke exposed rats. Chin J Physiol 1999 42(3) 137-143. 

ZO024 Park, K. K., K. S. Chum, ]. M. Lee, S. S. Lee, and Y. ]. Surh. Inhibitory effects of [6]-gingerol, a major pungent principle of ginger, on phorbol ester-induced inflammation, epidermal ornithine decarboxylase activity and skin tumor promotion in ICR mice. Cancer Lett 1998 129(2) 139-144. 

Luyengi, L. et al., Rotenoids and chalcones from Mundulea sericea that inhibit phorbol ester-induced ornithine decarboxylase activity. Phytochemistry, 36, 1523, 1994. 

After 7 days, lungs of high-dose group had elevated putrescine, spermidine, and ornithine decarboxylase activity, reflecting changes in polyamine metabolism no measurable effects in low-dose group (Dunbar et al. 1988b)... 

Oral treatment of adult female Sprague-Dawley rats with 425 mg/kg bw caprolactam 21 and 4 h before killing resulted in a significant increase in serum alanine aminotransferase activity (33%), while hepatic ornithine decarboxylase activity and cytochrome P450 content were not changed significantly (Kitchin Brown, 1989). 

Hydroquinone (1-10 (.miol/L) induced fluorescence from 2. 7 -dichlorofluorcscin acetate in HL-60 human leukaemia cells this was interpreted to indicate intracellular generation of hydrogen peroxide and other peroxides (Hiraku Kawanishi, 1996). Hydroquinone (200 mg/kg bw, as a single oral dose) administered to male Sprague-Dawley rats induced a three-fold increase in urinary excretion of malonaldehyde, increased hepatic ornithine decarboxylase activity from a control value of 16.8 pmol/mg/h... 

Ulrich S, Huwiler A, Loitsch S, Schmidt H, Stein JM. 2007. De novo ceramide biosynthesis is associated with resveratrol-induced inhibition of ornithine decarboxylase activity. Biochem Pharmacol 74 281-289. 

Ornithine decarboxylase activity in the brain reflects its pattern of... 

FIGURE 4 Metabolic pathway of polyamines. Increased levels of spermidine, spermine, putrescine, acetylspermidine, and acetylspermine without a change of ornithine in AD pathology were observed. One theory suggests that the NMDA receptor excitotoxicity is caused by an excess of spermidine and spermine due to ornithine decarboxylase activity induced by plaque and/or tangle deposition in specific brain regions. Reproduced from Ref. (112). 

Gupta, A.K., Fisher, G.J., Elder, J.T., Nickoloff, B.J., and Voorhees, J.J., Sphingosine inhibits phorbol ester-induced inflammation, ornithine decarboxylase activity, and activation of protein kinase C in mouse skin, J. Invest. Dermatol., 91, 486-491, 1988. 

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