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Organelle

Another possibility for extending the field of biosensors is to employ sections of animal or plant tissue as sources of enzymatic material. Tissue has the advantage of cohesion, and has a structure that is robust enough to be attached directly to the transducer without having to resort to protein immolnlization techniques. [Pg.21]

Plants are useful sources of enzymatic material for analytical chemistry. Using the appropriate transducer, very stable biosensors can be produced because the enzymes remain in their natural environment. Similarly, animal tissue can be considered as a useful bioreceptor for the selective detection of L-amino acids without any notable interference from D-amino acids. Sensor selectivity can also be improved by incorporation of an antimicrobial agent to avoid bacterial contamination. For example, it is recommended that 0.02 % sodium azide is used in the glutamine electrode [17]. [Pg.21]

Other biocatalysts are found in organelles such as lysosomes, chloroplasts, mitochondria and microsomes. These biomaterials have many en matic systems that can be exploited to construct biosensors. For instance, liver microsomes have a mpnooxygenase system with cytochrome P 4S0, which catalyses the hydroxylation of a large number of pharmaceutical derivatives, fatty acids, and steroidal [Pg.21]

Many cellular membranes can be excited by chemical stimuli which induce a conformational change in their chemoreceptors. These changes, and the functional modifications that result, are reversible and provide an interesting model for the construction of biosensors [16]. Neuroreceptors can also be used in the detection of drugs, toxins, and certain other substances [22]. [Pg.22]

The transducer provides the evidence that the reaction of the bioreceptor has occurred. The choice of transducer depends on the reaction type, and the substances liberated or consumed (Table 2.1). Generally, the choice is the appropriate commercialized transducer for the detection method required. There are a large number of electrodes available for electrochemical detection, for example, electrodes that are [Pg.22]


Ion Channels. The excitable cell maintains an asymmetric distribution across both the plasma membrane, defining the extracellular and intracellular environments, as well as the intracellular membranes which define the cellular organelles. This maintained a symmetric distribution of ions serves two principal objectives. It contributes to the generation and maintenance of a potential gradient and the subsequent generation of electrical currents following appropriate stimulation. Moreover, it permits the ions themselves to serve as cellular messengers to link membrane excitation and cellular... [Pg.279]

The DEP of numerous particle types has been studied, and many apphcations have been developed. Particles studied have included aerosols, glass, minerals, polymer molecules, hving cells, and cell organelles. Apphcations developed include filtration, orientation, sorting or separation, characterization, and levitation and materials handhng. Effects of DEP are easily exhibited, especially by large particles, and can be apphed in many useful and desirable ways. DEP effects can, however, be observed on particles ranging in size even down to the molecular level in special cases. Since thermal effects tend to disrupt DEP with molecular-sized particles, they can be controlled only under special conditions such as in molecular beams. [Pg.2010]

Biological membranes provide the essential barrier between cells and the organelles of which cells are composed. Cellular membranes are complicated extensive biomolecular sheetlike structures, mostly fonned by lipid molecules held together by cooperative nonco-valent interactions. A membrane is not a static structure, but rather a complex dynamical two-dimensional liquid crystalline fluid mosaic of oriented proteins and lipids. A number of experimental approaches can be used to investigate and characterize biological membranes. However, the complexity of membranes is such that experimental data remain very difficult to interpret at the microscopic level. In recent years, computational studies of membranes based on detailed atomic models, as summarized in Chapter 21, have greatly increased the ability to interpret experimental data, yielding a much-improved picture of the structure and dynamics of lipid bilayers and the relationship of those properties to membrane function [21]. [Pg.3]

These predictive methods are very useful in many contexts for example, in the design of novel polypeptides for the identification of possible antigenic epitopes, in the analysis of common motifs in sequences that direct proteins into specific organelles (for instance, mitochondria), and to provide starting models for tertiary structure predictions. [Pg.352]

Bioprocess The process that uses complete living cells or their components (e.g., enzymes, organelles, and chloroplasts) to effect desired chemical and/or physical changes. [Pg.901]

Neurons have three parts the cell body and dendrites, the axon, and axon terminals. The cell body contains the nucleus and the organelles needed for metabolism, growth, and repair. The dendrites are branched extensions of the cell body membrane. The axon is a long, thin structure which transfers electrical impulses down to the terminals. The axon divides into numerous axon terminals and it is in this specialized region that neurotransmitters are released to transmit information from one neuron to its neighbors. The synapse has been defined as the space between two subsequent interrelated neurons. ... [Pg.291]

Prokaryotic cells have only a single membrane, the plasma membrane or cell membrane. Because they have no other membranes, prokaryotic cells contain no nucleus or organelles. Nevertheless, they possess a distinct nuclear area where a single circular chromosome is localized, and some have an internal membranous structure called a mesosome that is derived from and continuous with the cell membrane. Reactions of cellular respiration are localized on these membranes. In photosynthetic prokaryotes such as the cyanobacteria,... [Pg.24]

Mitochondria Mitochondria are organelles surrounded by two membranes that differ markedly in their protein and lipid composition. The inner membrane and its interior volume, the matrix, contain many important enzymes of energy metabolism. Mitochondria are about the size of bacteria, 1 fim. Cells contain hundreds of mitochondria, which collectively occupy about one-fifth of the cell volume. Mitochondria are the power plants of eukaryotic cells where carbohydrates, fats, and amino acids are oxidized to CO9 and H9O. The energy released is trapped as high-energy phosphate bonds in ATR... [Pg.27]

Plant cells contain a unique family of organelles, the plastids, of which the chloroplast is the prominent example. Chloroplasts have a double membrane envelope, an inner volume called the stroma, and an internal membrane system rich in thylakoid membranes, which enclose a third compartment, the thylakoid lumen. Chloroplasts are significantly larger than mitochondria. Other plastids are found in specialized structures such as fruits, flower petals, and roots and have specialized roles. [Pg.29]

Plant cells also contain all of these characteristic eukaryotic organelles, essentially in the form described for animal cells. [Pg.29]

These organelles serve the same purposes in plant cells that they do in animal cells. [Pg.29]

Without consulting chapter figures, sketch the characteristic prokaryotic and eukaryotic cell types and label their pertinent organelle and membrane systems. [Pg.32]

Certain proteins endow cells with unique capabilities for movement. Cell division, muscle contraction, and cell motility represent some of the ways in which cells execute motion. The contractile and motile proteins underlying these motions share a common property they are filamentous or polymerize to form filaments. Examples include actin and myosin, the filamentous proteins forming the contractile systems of cells, and tubulin, the major component of microtubules (the filaments involved in the mitotic spindle of cell division as well as in flagella and cilia). Another class of proteins involved in movement includes dynein and kinesin, so-called motor proteins that drive the movement of vesicles, granules, and organelles along microtubules serving as established cytoskeletal tracks. ... [Pg.124]

Starch is stored in plant cells in the form of granules in the stroma of plas-tids (plant cell organelles) of two types chloroplasts, in which photosynthesis takes place, and amyloplasts, plastids that are specialized starch accumulation bodies. When starch is to be mobilized and used by the plant that stored it, it must be broken down into its component monosaccharides. Starch is split into its monosaccharide elements by stepwise phosphorolytic cleavage of glucose units, a reaction catalyzed by starch phosphorylase (Figure 7.23). This is formally an a(1 4)-glucan phosphorylase reaction, and at each step, the prod-... [Pg.228]

Cholesterol is a principal component of animal cell plasma membranes, and much smaller amounts of cholesterol are found in the membranes of intracellular organelles. The relatively rigid fused ring system of cholesterol and the weakly polar alcohol group at the C-3 position have important consequences for the properties of plasma membranes. Cholesterol is also a component of lipoprotein complexes in the blood, and it is one of the constituents oiplaques that form on arterial walls in atherosclerosis. [Pg.255]


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