Nude mouse human tumor models

Lactam-based analogs of epothilones were conceived by the BMS group as metabolically more stable alternatives to the lactone-based natural products (which exhibit limited metabolic stability in rodent plasma). It is worth noting, however, that despite its short plasma half-life in rodent species, Epo B shows potent antitumor activity in a variety of nude mouse human tumor models,and the same is tme for Epo D (vide infra). In addition, Epo D has subsequently been demonstrated to... 

Ossowski, L., Russo-Payne, H., and Wilson, E. L., Inhibition of urokinase-type plasminogen activator by antibodies The effect on dissemination of a human tumor in the nude mouse model. Cancer Res. 51, 274-281 (1991). 

Pimm, M. V., Durrant, L. G., and Baldwin, R. W. Influence of circulating antigen on the biodistribution and tumor localization of radiolabelled monoclonal antibody in a human tumor Nude mouse xenograft model. Eur. J. Cancer. Clin. Oncol. 25 1325-1332, 1989. 

A second animal model uses H-ros transgenic mice (Oncomouse ) which spontaneously develop mammary and salivary tumors.97 This model is considered to be more relevant to human cancer in that tumor initiation and growth is endogenous, in contrast to nude mouse tumor explants. Mice with palpable tumors between 50 and 350 mm3 were treated with either the ester prodrug 18c (40 mg/kg, once daily, s.c.) or vehicle, and the size of the tumors monitored over time.44 While... 

No rodent bioassay Tumor promotion potential was evaluated by first identifying human tumor cell lines that bind natalizumab in vitro Tumor promotion potential was then evaluated by assessing tumor growth in athymic nude mice after tumor transplantation (sc) followed by treatment with natializumab. Natalizumab did not show any tumor promoting potential on two oc4 expression tumors (leukemia, melanoma) in a nude mouse xenograft model Not genotoxic (Ames, in vitro chromosomal aberrations in human PBLs)... 

Carcinogenicity Tg.rasH2 transgenic mouse model Other antigenicity in guinea pigs, mice, and rats tumor cell lines (effects of the product on proliferation) human tumor xenografts in athymic nude mice hemolytic potential studies... 

An important advance in preclinical models for anticancer agents was the development of immuno-suppressed mouse strains that allowed for the reproducible implantation and growth of human tumor cells in vivo. The first xenograft of a human colon cancer cell line into immunocompromised "nude" mice was reported in 1969 by Rygaard and Povlsen (41). 

As mentioned above, drug potency is a body weight independent parameter, and it should be the same across species. Therefore, Vk2 obtained from a mouse model can be used to predict the total AUC during the mean tumor growth time in humans. It was demonstrated that Vk2 obtained from human tumor xenografts in nude mice correlates to total AUC in a three-week cycle in human for 10 anticancer agents at their average therapeutic dose [55],... 

Park B, Prasad S, Yadav V, Sung B, Aggarwal BB (2011) Boswellic acid suppresses growth and metastasis of human pancreatic tumors in an orthotopic nude mouse model through modulation of multiple targets. PloS One 6 e26943... 

Fig. 9 Fluorescence imaging of lymphocytes in mice. Labeled and/or engineered cells were administrated intravenously (n = 3, 100 pL/mouse, lO cells) and whole body was scanned liom the back side by eXplore Optix, GE Healthcare, Bioscience (excitation at 646 nm, anission 663 nm), 1 h, 2 h, 4 h, 6 h, 48 h, and 1 week after injection. Data woe normalized. SP spleen, LN lymph node of epidermal intestinal tract TM DLD-1 human colon carcinoma, (a) Cy5-labeled cells to the nude mice, (b) Cy5-labeled cells to the DLD-1 implanted nude mice at dorsal division, (c) Both Cy5-labeled and Al-glycan-engineered lymphocytes into the tumor model...

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