Neurospora crassa genes

Springer ML, Yanofsky C. Expression of con genes along the three sporulation pathways of Neurospora crassa. Genes Dev. 1992 6 1052-7. doi 10.1101/gad.6.6.1052. 

Neurospora crassa is a filamentous pink mould. It became famous to scientists due to the work of Beadle and Tatum in the 1940s when they developed the one gene—one enzyme hypothesis. Its life cycle is shown in Fig. 2.9. Unforturrately, the full force of fungal nomenclature comes into play when considering this organism. Aerial hyphae... 

Bieszke, J. A., E. L. Braun et al. (1999). The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins. Proc. Natl. Acad. Sci. 96(14) 8034-8039. 

Bai, C., et ak, SKPl connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cdl, 1996, 86(2), 263-74. Kumar, A. and J. V. Paietta, The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with -transducin repeats. 

DIMS was identified as the responsible gene for one of DNA hypomethylation mutants in Neurospora crassa (Tamaru and Selker, 2001). This is the first report that histone (H3K9) methylation regulates DNA methylation. Furthermore, it is reported that this regulation is mediated through the HPl recruitment to the tri-methylated H3K9 loci (Freitag et al, 2004), but it remains to be elucidated whether DNA methylation (DNA methyltransferase) is directly controlled (recruited) by HPl. 

The gene mutation test in Neurospora crassa US-EPA OPPTS 870.5250... 

The test is used to detect forward and reverse gene mutation in Neurospora crassa, a eukaryotic fungus. These mutations are detected by biochemical or morphological changes in the treated population. The most commonly used mutation assay in Neurospora crassa measures forward mutation in the ad-3 region of the genome. 

Laurila, M. R., Salgado, P S., Makeyev, E. V., Nettleship, J., Stuart, D. I., Grimes, J. M. and Bamford, D. H. (2005). Gene silencing pathway RNA-dependent RNA polymerase of Neurospora crassa yeast expression and crystalUzation of selenomethionated QDE-1 protein. J. Struct. Biol. 149,111-115. 

Metallothioneins from Neurospora crassa contain only 25 amino acid residues, but the primary structure is quite similar to the N-terminal part of the mammalian proteins.1454 These data indicate that the gene that codes for the Neurospora crassa metallothionein is evolutionary related to the gene of the vertebrate metallothioneins. Equine metallothionein exists in two major variants (metallothionein 1A and metallothionein 2A), which show remarkable similarities. Some allelic polymorphic variants also occur in man, horse and rabbit. Recent developments have been comprehensively reviewed.14678... 

Munger, K., Germann, U.A. Lerch, K. (1987). The Neurospora crassa metallothionein gene. Journal of Biological Chemistry 262, 7363-7. 

Fu, Y.-H., Kneesi, Y.Y. Marzluf, G.A. (1989). Isolation of Nit-4, the minor nitrogen regulatory gene which mediates nitrate induction in Neurospora crassa. Journal of Bacteriology 171, 4067-70. 

Fu, Y.-H. Marzluf, G.A. (1990). nit-2, the major positively-acting nitrogen regulatory gene of Neurospora crassa, encodes a sequence-specific DNA-binding protein. Proceedings of the National Academy of Sciences (USA) 87, 5331-4. 

Okamoto, P.M., Fu, Y.-H. Marzluf, G.A. (1991). Nit-3, the structural gene of nitrate reductase in Neurospora crassa nucleotide sequence and regulation of mRNA synthesis and turnover. Molecular and General Genetics 227, 213-23. 

Genes for pyruvate decarboxylases have been isolated from yeasts and fungi such as Saccharomyces cerevisiae [86-90], Hanseniaspora uvarum [91], Klyveromyces marxianus [92], Klyveromyces lactis [93], Neurospora crassa [94], Aspergillus parasiticus [95], plants, like maize (Zea maize) [96,97], rice (Oryza sativa) [98], tomato (Lycopersicon esculentum) [99,100], pea (Pisum sativum) [101], and tobacco (Nicotiana tabacum) [102] and from the bacterium Zymomonas mobilis [103-106]. 

Kuper, U., Niedermann, D. M., Travaglini, G. and Lerch, K. (1989). Isolation and characterization of the tyrosinase gene from Neurospora crassa. J. Biol. Chem., 264, 17250-17258. 

Fungi Mutation Neurospora crassa ad-3 system red adenine mutants Gene (forward) mutations and small deletions in ad3A and ad3B <2 mo M M M L... 

Mutation Neurospora crassa Reversion of adenine auxo-trophy in strains N23 and N24 Gene mutations <1 mo L L S NA... 

Katz, T-M. Ong, A.J.F. Griffiths, and R.S. Stafford. Mutation tests in Neurospora crassa. A report for the U.S. EPA GENE-TOX Program. Mutat. Res. (in press)... 

Cu-induced transcriptional activation of metallothionein is known in Neurospora crassa, hut the factor that mediates Cu activation is unknown (Munger el a/., 1987). The MT gene does not contain an Acel-hinding site in its 5 promoter sequences. 

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