Neuroblastoma SY5Y, cell line

GO Human neuroblastoma SH-SY5Y cell line <80 pg mL" 48 and 96 h No obvious cytotoxicity 146... 

SH-SY5Y neuroblastoma, cisplatin-sensitive A2780 and cispla-tin-resistant A2780cis human ovarian cancer cells was observed, but upon irradiation 7 strongly reduced the viability of the cancer cells (Fig. 8). In the A2780 cell line, the complex was 80x more toxic than cisplatin under identical conditions, and ca. 15 x more effective against the cisplatin-resistant A2780cis cell line (33). The trans diazido-Pt(IV) complex therefore has remarkable cytotoxic properties. 

Newton RA, Phipps SL, Flanigan TP, Newberry NR, Carey JE, Kumar C, McDonald B, Chen C, Elliott JM. (1996). Characterisation of human 5-hydroxytryptamine2A and 5-hydroxytryptamine2C receptors expressed in the human neuroblastoma cell line SH-SY5Y comparative stimulation by hallucinogenic drugs. J Neurochem. 67(6) 2521-31. 

Manzamine A was able to block the cytosolic calcium increase induced by KCl depolarization on human neuroblastoma SH-SY5Y cells. The maximum effect was observed at 1.0 jxM, inhibiting by 31.5%. 8-Hydroxymanzamine A also showed a similar effect whereas manzamines E, F, and Y, ircinal A and weo-kauluamine did not show any significant effect [44]. In the B lymphocytes reaction assay hyrtiomanza-mine showed immunosuppressive activity with an EC50 of 2.0 xg/mL, but no activity was displayed against the KB human epidermoid carcinoma cell line [53]. 

The simplest in vitro model, the cell line, is a population of cells that can be maintained in culture for an extended period of time. Neuronal cell lines normally originate from a single common ancestor cell (clonal) and are often derived from tumors, e.g., pha-eochromocytomas (adrenal medullary tumor) (e.g., PC12 cell line) [13] and neuroblastomas [14] such as mouse N2a or human SH-SY5Y. However, a recently developed technique to introduce oncogenes into primary cultures through retroviruses has opened up new possibilities [15]. 

Discovery Epolactaene (1) was isolated from the fungal strain Penicillium sp. BM 1689-P as a neuritogenic compound that induced neurite outgrowth in the human neuroblastoma cell line SH-SY5Y, which lacks significant TRK family mRNAs [1],... 

NB4I43 mouse neuroblastoma and SK.-N-SH-SY5Y human neuroblastoma cell lines differentiated with retinoic acid (Ehrich etal.. J997)... 

Low levels (0.1-1 pg/mL) of resveratrol have been reported to enhance cell proliferation, whereas higher amounts (10-100 pg/mL) cause apoptosis in a vari-ety of tumor and endothelial cells, including JB6 epidermal cells, human promyeloctye leukemia HL-60 cells, THP-1 human monocytic leukemia cells, U937 human promonocytic cells, various colon cancer cell lines, human mammary cancer cell lines, and human prostate cancer cells [121-131]. In somewhat of a contrast to these studies, a very recent report indicated that resveratrol reduced paclitaxel-induced apoptosis in a human neuroblastoma cell line (SH-SY5Y) by blocking paclitaxel-induced phosphorylation of JNKs, Raf-1, and BcT2 [132]. Of particular interest is a recent study in which it was shown to induce apoptosis in leukemic human lymphocytes but, under similar conditions, had no effect on the survival... 

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