Escherichia coli plasmids

Rodriguez H, Loechler EL. 1993. Mutational specificity of the (+) - anti-diol epoxide of benzo [a] pyrine in a supF gene of an Escherichia coli plasmid DNA sequence context influences hotspots, mutagenic specificity and the extent of SOS enhancements of mutagenesis. Carcinogenesis 14(3) 373-383. 

Ingmer, H. Miller, C.A. Cohen, S.N. Destabilized inheritance of pSClOl and other Escherichia coli plasmids by DpiA, a novel two-component system regulator. Mol. Microbiol., 29, 49-59 (1998)... 

As an example, we use RNAI, an antisense repressor of the replication of some Escherichia coli plasmids with a ColEl origin of replication. 

Walsh, M.K. and Swaisgood, H.E. 1994. An Escherichia coli plasmid vector system for production of streptavidin fusion proteins expression and bioselective adsorption of streptavidin-(3-galactosidase. Biotechnol. Bioengng 44, 1348-1354. 

The structure of the bacterial ArsC from Escherichia coli plasmid R773 has been solved at 1.65 A resolution, and revealed that arsenate reductase (ArsC) has only one cysteine residue (Cys-12) in the active site, surrounded by an arginine triad composed of Arg-60, Arg-94, and Arg-107 (Mukhopadhyay et al, 2002). However, the arsenate reductase from Staphylococcus aureus has three cysteine residues (Cys-10, Cys-82, and Cys-89). The biochemical and mutational studies established that the arsenate binds to the triad of arginine (Arg-60, Arg-94, and Arg-107) residues and forms a covalent bond with the cysteine (Cys-12) residue near the N-terminus at the active site of arsenate reductase and/or participates in catalysis (Rosen, 2002a Silver and Phung, 2005 Shi etal, 2003 Martin et al, 2001). 

Escherichia coli K12 TGI strain was used as a recipient for transformation. At studying of SOS-system activity the recombinant bioluminescent strain of Escherichia coli recA lux containing plasmid-borne fusions of the recA promoter-operator region to the Photorhabdus luminescens ZM 1 lux genes (GosNlIgenetika, Russia) was used. Increase of their luminescence in the presence of DNA damage factors [Rosen et al., 2000], were shown previously. Investigation of the luminescent response of this strain to UV radiation allows quantitatively estimate in a real time a SOS-system induction. 

Recombinant DNA technology can also be used to design genes that encode for proteins with desired features [34]. The gene can be incorporated into a plasmid, which is then used to transform a bacterial host such as Escherichia coli. Finally, the production of the desired amino acid polymer is performed by the host with a precisely defined sequence and near absolute monodispersity [29, 35]. 

Ruther, A. et al., Production of zeaxanthin in Escherichia coli hansformed with different carotenogenic plasmids, Appl. Microbiol. Biotechnol. 48, 162, 1997. 

The degradation of phenylmercuric acetate to benzene, methylmercuric chloride to methane, and ethylmercuric chloride to ethane and Hg + is apparently carried out by different enzymes from the plasmid-carrying Escherichia coli strain K12 (R831) (Schottel 1978) and Pseudomonas sp. Resistance to organic mercury compounds has also been found in clinical isolates of nontuber-culous, rapidly growing mycobacteria (Steingrube et al. 1991) and can present a challenge in the clinical environment. 

Schottel JL (1978) The mercuric and organomercurial detoxifying enzymes from a plasmid-bearing strain of Escherichia coli. J Biol Chem 253 4341-4349. 

A study with a strain of plasmid-bome antibiotic-resistant Escherichia coli indicated that the strain did not transmit these plasmids to indigenous strains after introduction into the terrestrial environment (Devanas et al. 1986). 

Devanas MA, D Rafaeli-Eshkol, G Stotsky (1986) Survival of plasmid-containg strains of Escherichia coli in soil effect of plasmid size and nutrients on survival of hosts and maintenance of plasmids. Curr Microbiol 13 269-277. 

Stalker DM, KE McBride (1987) Cloning and expresssion in Escherichia coli of a Klebsiella ozaenae plasmid-borne gene encoding a nitrilase specific for the herbicide Bromoxynil. J Bacterial 169 ... 

Fig. 1 Schematic outline of procedures employed in the synthesis of a cDNA gene copy from a polyadenylated mRNA template, insertion of the cDNA into a bacterial plasmid vector by a homopolymer tailing strategy, and cloning of the recombinant plasmid in an Escherichia coli host.

Metcalf, W. W., Jiang, W., and Wanner, B. L. (1994). Use of the rep technique for allele replacement to construct new Escherichia coli hosts for maintenance of R6K gamma origin plasmids at different copy numbers. Gene 138, 1-7. 

Bouanchaud DH, Hellio R, Bieth G, et al. 1975. Physical studies of a plasmid mediating tetracycline resistance and hydrogen sulfide production in Escherichia coli. Mol Gen Genet 140(4) 355-359. 

An (5)-specific alcohol dehydrogenase gene from Rhodococcus erythropolis and GDH from Bacillus subtilis were ligated into one plasmid, which was expressed in Escherichia coli strain DSM14 459 to provide an (S)-selective whole-cell catalyst. 

An (R)-selective counterpart was constructed in the same manner by using Escherichia coli DSM14 459 as host organism, but in this case two separate plasmids were used, which contain... 

Muller, J., Dijl, J.V., Venema, G. and Bron, S. (1996) Cloning of heterologous genes specifying detrimental proteins on pUC-derived plasmids in Escherichia coli. Molecular Genetics and Genomics, 252, 207-211. 

Denis-Larose, C. Bergeron, H. Labbe, D., et al., Characterization of the Basic Replicon of Rhodococcus Plasmid pSOX and Development of a Rhodococcus Escherichia Coli Shuttle Vector. Applied and Environmental Microbiology, 1998. 64(11) pp. 4363-4367. 

Nataro JP, Yikang D, Giron JA, Savarino SJ, Kothary MH, Hall R Aggregative adherence fimbria I expression in enteroaggregative Escherichia coli requires two unlinked plasmid regions. Infect Immun 1993 61 1126-1131. 

Navarro-Garcia F, Canizalez-Roman A, Luna J, Sears C, Nataro JP Plasmid-encoded toxin of enteroaggregative Escherichia coli is internalized by epithelial cells. Infect Immun 2001 69 1053-1060. 

Nataro JP, Seriwatana J, Fasano A, Maneval DR, Guers LD, Noriega F, Dubovsky F, Levine MM, Morris JG Jr Identification and cloning of a novel plasmid-encoded enterotoxin of enteroinvasive Escherichia coli and Shigella strains. Infect Immun 1995,63 4721-4728. 

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