Human melanoma cell line

K8. Kirchheimer, J. C., Wojta, J., Christ, G., and Binder, B. R., Functional inhibition of endogenously produced urokinase decreases cell proliferation in a human melanoma cell line. Proc. Natl. Acad. Sci. U.S.A. 86, 5424-5428 (1989). 

The polyhalogenated monoterpenes 57-59 from the Spanish sea hare Aplysia punctata show identical cytotoxic properties against P-388 mice lymphoma and HT-29 human colon carcinoma (ED50 2.5 pg/ml), A-549 human lung carcinoma and MEL-28 human melanoma cell lines (ED50 1.5 pg/ml) [57]. 

NGF (nerve growth factor) — required for maintenance of neuronal differentiation. Obtained from human melanoma cell line A37S. 

In a similar way, sarcoma growth factors (SGFs e.g. ESG) can be obtained by the serum-free cultivation of murine sarcoma virus transformed 3T3 cells or transformed rat kidney cells. Nerve growth factor (NGF) is produced in excess by the human melanoma cell line A375 and fibroblast growth factor (FGF) is secreted by fibrosarcoma cells (Todaro et al., 1979). 

Boon MH, Parsons PG. 1984. Cyclophosphamide resistance developed in a human melanoma cell line. Cancer Treat Res 68 1239-1246. 

Dysbindin-1 has been localized immunohistochemically in a human melanoma cell line (MNT-1), mouse skeletal muscle, and the mouse and human CNS. 

Bani, M. R., Rak, J., Adachi, D., Wiltshire, R., Trent, J. M., Kerbel, R. S. and Ben-David, Y. (1996). Multiple features of advanced melanoma recapitulated in tumorigenic variants of early stage (radial growth phase) human melanoma cell lines evidence for a dominant phenotype. Cancer Res. 56, 3075-3086. 

Gutman M, Singh RK, Radinsky R, et al. Intertumoral heterogeneity of receptor-tyrosinase kinase expression in human melanoma cell lines with metastatic capabilities. Anticancer Res. 1994 14 1759-1765. 

Trichodermatides A—D (66—69) have been characterized from the fungus Trichoderma reesei, obtained from marine sediments in China. Octaketide derivatives such as 67—69 with an 0 ,/ -unsaturated cyclohexenone fused to a pyran ring have repeatedly been reported from the genus Trichodermabut do not seem to occur elsewhere in nature. 66 is the first example of a pentacyclic polyketide with a ketal moiety. 66—69 exhibited weak cytotoxicity toward the A375-S2 human melanoma cell line. 

In particular, LIF appears to be identical with HSF-III (hepatocyte-stimulating factor III), which is known to stimulate the synthesis of acute phase plasma proteins (Baumann and Wong, 1989), and with MLPLI (melanoma-derived lipoprotein lipase-inhibitor) which is produced in a human melanoma cell line, SEKI. LIF inhibits lipoprotein lipase activity in adipocytes and possibly causes cachexia (Mori et al., 1989). 

Q. Peng, G.W. Farrants, K. Madslien, J.C. Bommer, J. Moan, H.E. Danielsen, J.M. Nesland (1990). Subcellular localization, redistribution and photobleaching of sulfonated aluminum phthalocyanines in a human melanoma cell line. Int. J. Cancer, 49, 290-295. 

Mammalian cells produce two t-PA variants of N-linked glycosylation, type 1 (at asparagines 117,184, and 448) and type 2 (only as asparagines 117 and 448). The rate of fibrin-dependent plasminogen activation is two- to threefold faster for type 2 compared with type 1. The cDNA obtained from a human melanoma cell line was expressed in CHO cells to achieve glycosylation and a protein identical to the natural protein. Protein engineering studies have produced variant t-PA molecules with modified pharmacokinetics, affinity tor fibrin, catalytic activity, and side effects. 

Many of these terpenoids have been reported to be biologically active as indicated in Table 1, although such work has occurred on these compounds when isolated as constituents of plant families other than the Dipterocarpaceae [68-81]. For example, the common triterpenoid, betulinic acid (21), was reported as having selective cytotoxic activity for several human melanoma cell lines, and also demonstrated highly effective tumor growth inhibition in athymic (nude) mice with human melanoma cells at non-toxic doses [72]. 

Sanna K, Rofstad EK (1994) Hypoxia-induced resistance to doxorubicin and methotrexate in human melanoma cell lines in vitro. Int J Cancer 58 258-262... 

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