Multiple antigen peptide -system

Tam, J.P. (1988) Synthetic peptide vaccine design Synthesis and properties of a high-density multiple antigenic peptide system. Proc. Natl. Acad. Sci. USA 85, 5409-5413. 

Applications of the TASP approach are then considered for such aspects as four-helical bundles (Section 13.1.2.4.2)J4 The details of structures and synthetic routes for the preparation of the bundles are described. Other chain assemblies and applications are discussed (Section 13.1.2.5) including metal-ion assisted self assemblies, disulfide linkages in the formation of four-helical bundles (Section 13.1.2.5.2), multiple antigenic peptide systems (Section 13.1.2.5.4), and templates constructed from a trialdehyde template by reductive amination to form a three-helical bundle (Section 13.1.2.5.5))5 ... 

High-Density Multiple Antigenic Peptide Systems... 

We have demonstrated that peptides which differ in their C-tennini can be simultaneously synthesized in one reaction vessel by employing resins that possess different cleavage properties. This synthesis, strategy can also be used for the synthesis of multiple antigenic peptide systems, MAPS (8) and their corresponding des-lysine core sequences. Moreover, this strategy can be expanded to the synthesis of more than two peptides by employing other resins such as the HF cleavable Pam resins and the photo-labile resins. 

Coupling of lipids to peptide antigens may be performed directly or via an amplifying dendrimer carrier system. A commonly investigated carrier is the multiple antigen peptide (MAP)-system, which connects peptide antigens through a branched polylysine core. 

Several peptide dendrimers have been reported/ a major application of which is for the preparation of multiple antigen peptides (MAPs). For example, Tam used the poly (lysine) platform to prepare a MAP. These peptides are used to activate the immune system to produce large numbers of antipeptide antibodies, and their use avoids the immunogenicity and the other disadvantages associated with conventional antigenic systems. 

Abbreviations IgGl. immunoglobulin Gl KLH, keyhole limpet hemocyanin BSA, bovine serum albumin RNase A. ribonuclease A LDH. lactate dehydrogenase MHC, major histocompatibility complex LHRH, luteneizing hormone releasing hormone CCK. cholecystokinin VIP. vasoactive intestinal peptide TASP, template-assembled synthetic proteins MAPS, multiple antigen presenting system SUV, small unilamellar vesicle DCC. dicyclohexylcarbodiimide HOSu. N-hydroxysuccinimide Mal>. maleimido TFA, trifluoroacelic acid TFE. triiluoroethanol DMF, dimethylformamide DMSO. 

The lipid-core peptide (LCP) system (Toth et al. 1993 Moyle et al. 2003) (Fig. 11.3) is a delivery system which conjugates synthetic lipoamino acids (a-amino acids with long alkyl side chains) through a polylysine MAP system (or a carbohydrate) (McGeary et al. 2001, 2002) to multiple copies of one or several different peptide antigens. The LCP system induces similar immune responses when LCP-based vaccines are co-administered with conventional adjuvants and represents a promising system for mucosal vaccine development. 

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