Antibodies antipeptide

The small luminal loop in the proposed structure of Ca -ATPase [11] between transmembrane helices 7 and 8 (residues 870-890) was suggested to contain the epitope for mAb A20 [139], and for an antipeptide antibody directed against the 877-888 sequence (TEDHPHFEGLDC) of the Ca -ATPase [138],... 

Petralia, R. S., Wang, Y. X., and Wenthold, R. J. (1994) Histological and ultrastructural localization of the kainate receptor subunits, KA2 and GluR6/7, in the rat nervous system using selective antipeptide antibodies. J. Comp. Neurol. 349, 85-110. 

Brass, M., Hammermann, R., Brimijoin, S., and Bonisch, H. (1995) Antipeptide antibodies confirm the topology of the human norepinephrine transporter. J. Biol. Chem. 270,9197-9201. 

Like FRET, today BRET is predominantly used in biological sciences, especially in the monitoring of protein-protein interactions such as hormone-receptor interaction [223, 224] and protein-DNA interaction in living systems. However, BL resonance energy transfer can also be applied in immunoassays by using for instance a peptide-tagged luciferase and a fluorescein-labeled antipeptide antibody [225]. The development of more BRET assays for small-molecule analytes is thus awaited. 

CYPlAl appears to be expressed at very low levels (if at all) in human liver. Wrighton and Stevens (1992) report that only one of 50 human liver samples contained protein immunoreactive to an antibody raised against rat CYPlAl. Murray et al. (1993) used CYPlAl-specific and CYPlA2-specific antipeptide antibodies and found no evidence for CYPlAl expression in human liver microsomes. However, McKinnon. (1991) reported detection of CYPlAl mRNA in 11 of 23 human liver samples studied. 

Ransnas, L. A., and Insel, P. A. (1988). Quantitation of the guanine nucleotide binding regulatory protein Gs in S49 cell membranes using antipeptide antibodies to alphas. / Biol. Chem. 263, 9482-9485. 

Birkenberger, L., and Ip, W. (1990). Properties of the desmin tail domain Studies using synthetic peptides and antipeptide antibodies./. Cell Biol. Ill, 2063-2075. 

Ishii, Y., Takami, A., Tsuruda, K., Kurogi, A., Yamada, H., and Oguri, K. (1997). Induction of two UDP-glucuronosyltransferase isoforms sensitive to phenobarbital that are involved in morphine glucuronidation. Production of isoform-selective antipeptide antibodies toward UGTl.lr and UGT2B1. Drug Metab. Dispos. 25 163-167. 

The methods described are suitable for use with immunogens derived from a variety of sources, for example, bacterially expressed fusion proteins, baculovirus-expressed proteins or synthetic peptides conjugated to suitable carrier proteins (see Chapter 2). Antibody responses to peptide immunogens often differ from those where the immunogen is a larger macromolecule in that maximal antipeptide titres (which arise rapidly after two to three immunizations) do not always coincide with maximal titres against the intact... 

The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way. 

Because synthetic peptide immunogens used to generate antipeptide antibodies often are available in comparatively large... 

Stresser DM, Kupfer D. Monospecific antipeptide antibody to cytochrome P-450 2B6. Drug Metab Dispos 1999 27 517-525. 

Basu SK, Shen J, Elbert KJ, et al. Development and utility of anti-PepTl antipeptide polyclonal antibodies. Pharm Res 1998 15 338-342. 

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