Mouse plasma extract

H. M. M. Arafa, E. M. A. Hamada, M. M. A. Elzamai and H. Nau, Eully automated detemination of selective retinoic acid receptor ligands in mouse plasma and tissue by reversed-phase liquid chi omatography coupled on-line with solid-phase extraction , 7. Chromatogr. A 729 125-136 (1996). 

Ulrich, B. Frey, F.J. Speck, R.F. Frey, B.M. Pharmacokinetics/pharmacodynamics of ketoconazole-prednisolone interaction. J.Pharmacol.Exp.Ther, 1992, 260, 487-490 [mouse plasma dexamethasone (IS) LOD 10 ng/mL extracted prednisolone]... 

Figure 6.3 Representative two-dimensional mass spectrometric analyses of lysoPC molecular species in Upid extracts of mouse plasma in the positive-ion mode. A full MS scan in the mass range from m/z 460 to 610 was acquired first from a diluted hpid extract of mouse plasma in the positive-ion mode, which displayed the abundant sodium adducts of lysoPC species. Neutral loss scan (NLS) of 59.0 amu (i.e., trimethylamine) with a collision energy (CE) of 22 eV or 205 amu (i.e., sodium chohnephosphate, CE 34 eV), precursor-ion scan (PIS) of m/z 104.1 (i.e., choline, CE 34 eV), and PIS of m/z 147.0 (i.e., sodiated five-membered cyclophosphane, CE 34 eV) with collision-gas pressure at 1 mTorr were also acquired from the diluted hpid extract of mouse plasma in the positive-ion mode. All scans were displayed after normalization to the base peak in individual scan.

Historically, the development of animal cell culture systems has been dependent upon the development of new types of tissue culture media. Mouse L cells and HeLa cells were developed using a balanced salt solution supplemented with blood plasma, an embryonic tissue extract, and/or serum. In 1955 Eagle developed a nutritionally defined medium, containing all of the essential amino acids, vitamins, cofactors, carbohydrates, salts, and small amounts of dialyzed serum (Table 1). He demonstrated that this minimal essential medium (MEM) supported the long-term growth of mouse L and HeLa ceils. Eagle s MEM was so well defined that the omission of a single essential nutrient eventually resulted in the death of these animal cells in culture. 

One of the challenges in the determination of lovastatin and its P-hydroxy acid in mouse and rat plasma was the small amount (0.1 ml) of plasma available [46], The analytes were isolated from plasma by SPE. The reconstituted extracts were separated using a 5Qx2-mm-lD Cig column (5 pm) and 1 mmol/1 aqueous AmOAc (pH 4.0)-acetonitrile gradient. They were analysed by ESl-MS in SRM mode with polarity switching, i.e., in positive-ion mode for lovastatin and in negative-ion mode for its P-hydroxy acid. Simvastatin and its P-hydroxy acid were used as ANIS. An LOQ of 0.5 ng/ml was achieved with intra-day and inter-day precision better than 7%. The method was vahdated. 

At the same time, the bioanalysis of LOR and DCL in rat, rabbit, mouse, and dog plasma was reported by others [64]. In order to get more rehable toxicology data, the bioanalysis in these four preclinical species is done simultaneously instead of on separate days. The sample pretreatment was SPE in a 96-well plate format, using a Tomtec Quadra hquid handling system and an Empore Cig 96-well extraction disk plate. Fom-channel parallel LC was done with four 100x2-mm-lD Cg colunms (5 pm) and a mobile phase of 85% methanol in 25 mmol/1 aqueous AmOAc (adjusted to pH 3.5). The mobile phase was delivered at a flow-rate of 800 pl/min and split into 200 pl/min over each of the four colunms. A multi-injector system was apphed with four injection needles. A post-column spht was applied to deliver 60 pEmin per column to a four-channel multiplexed ESI source (Ch. 5.5.3). The interspray step time was 50 ms. Positive-ion ESI-MS was performed in SRM mode with a dwell time of 50 ms for each of the four transitions, i.e., LOR, DCL, and their [DJ-ILIS, with 20 ms interchannel delay. The total cycle time was thus 1.24 s. The LOQ was 1 ng/ml for both analytes. QC samples showed precision ranging from 1 to 16% and accuracy from -8.44 to 10.5%. The interspray crosstalk was less than 0.08% at concentrations as high as 1000 ng/ml. 

Partially purified extracts of brain also induce cholinergic traits in sympathetic neurons (Kessler et al., 1986) and this is facilitated by plasma membranes and membrane-bound factor (Lee et al., 1990). Depolarization stimulates CAT in mouse spinal cord cultures (Ishida and Deguchi,... 

The modified polymer beads [347] passed all of the standard battery of biocompatibility tests required by the International Organization for Standardization guidelines (ISO 10993). The tests included in vitro coagulation tests (plasma recalcification time), hemolysis study (extraction method), cytotoxicity study using the ISO elution method, etc. In in vivo experiments, extracts of the polymer beads did not elicit pyrogenic irritation or sensitization reactions in laboratory animals (acute systematic toxicity study in the mouse, acute intracutaneous reactivity study in the rabbit, rabbit pyrogen study). 

New still considered heterologous sera unsatisfactory for embryo culture. In a previous study (New and Stein, 1964) in which mouse and rat embryos were cultured on clots of plasma and embryo extract, it was interesting to note that development was eomparable with embryo extraet prepared from 13-day chick embryos or from the entire uterine contents of 17-19-day pregnant mice. 

The following protocol has been used in the analysis of steroids extracted from cerebrospinal fluid (CSF), plasma, brain (see the sections Plasma for ESI-MS and Tissue [Mouse Brain] for ESI-MS ), and cell culture [58,67,74]. Volumes and column sizes have been optimized for steroid extracts from 500 pL CSF, 100 pL plasma, and 50 mg rat brain. Isotope-labeled internal... 

Wu Y, Zhao J, Hettion JD, Korfmacher WA, Lpaiguera AP, Lin C-C. Microsample determination of lovastatin and its hydroxy acid metabolite in mouse and rat plasma by liquid ehromatography-ionspray tandem mass spectrometry. J Mass Spectrom. 1997 32 379-87. Zhao JJ, Xie IH, Yang AY, Roadcap BA, Rogers JD. Quantitation of simvastatin and its beta-hydroxy acid in human plasma by liquid-liquid cartridge extraction and liquid chromatography-tandem mass spectrometry. J Mass Spectrom. 2000 35 1133-43. 

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