Mouse embryonic

The biological impact of starch capped copper nanoparticles on mouse embryonic fibroblast (3T3L1) cells in vitro) was also evaluated by various parameters. More than 85 % of the 3T3Llcells were found to be viable, even after 20 hours time exposure which implies minimum impact on cell viability and morphology. The study demonstrates dose dependent cytotoxic potential of SCuNPs, that is non cytotoxic in the nanogram dose and moderately cytotoxic in the microgram doses (Fig. 10). Comparison of SCuNPs with Cu ions and uncapped copper nanoparticles (UCuNPs) revealed that, ions are more cytotoxic than SCuNPs. This observation supports the theory of slow release of ions from starch coated nanoparticles. 

Fig. 10. Effect of starch capped copper nanoparticles on cell viability (MTT assay) in case of mouse embryonic fibroblast (3T3L1).

Fig. 11. Photomicrographs of mouse embryonic fibroblast (3T3L1) cells (A) untreated, treated with (B) control (C) SOOng CuNPs, (D) lOOOng CuNPs.

Nemeth KR, Charlap JH, O Hara MF, Craig RC, Knudsen TB. 2002. Microarray analysis of developmental toxicity ontogenetic profiles of susceptibility in the mouse embryonic eye. Toxicologist 66 28. 

Zhu, L. et al. (2007) DNA damage induced by multiwalled carbon nanotubes in mouse embryonic stem cells. Nano Letters Issues, 7 (12), 3592-3597. 

It is well known that 1,10-phenanthrolines are highly active ironchelating agents. The parent compound itself has recently been shown to increase HIF-la levels in ocular tissue and to suppress 02-mediated epithelial cell proliferation when administered to mice [29]. A quantitative assay was developed to measure transcriptional potency of certain HIF stabilizers via an HRE-mediated (3-lactamase production in which the EC50 of 1,10-phenanthroline was measured to be approximately 8 pM. In addition, VEGF was dose-dependently produced in mouse embryonic fibroblasts by 1,10-phenanthroline with an EC50 of... 

Fig. 13 Phase contrast microscope images of mouse embryonic stem cells in the PMBV/PVA hydrogel left) and on TCPS (right)...

Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126 663-676... 

Stavridis, M. P. and Smith, A. G. Neural differentiation of mouse embryonic stem cells. Biochem. Soc. Trans. 31 45-49, 2003. 

Kanellopoulou C, Muljo SA, Kung AL, Ganesan S, Drapkin R, Jenuwein T, Livingston DM, Rajewsky K (2005) Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing. Genes Dev 19 489—501... 

Found in microsomal fraction of rat and mouse embryonic but not adult CNS (Compagnone et al., 1995). 

In vitro studies with unmodified and modified N-terminal peptides of H3 demonstrated that Lys-14 acetylation did not interfere with methylation at Lys-9 by Suv39hl, while phosphorylation at Ser-10 and acetylation at Lys-9 did (Fig. 7). Further dimethylation of Lys-9 reduced enzymatic activity [186], A Suv39h double null primary mouse embryonic fibroblasts had higher levels of Ser-10 phos-phorylated H3 than wild type cells. These mutant cells had increased numbers of micro- and polynuclei. Oversized nuclei were characteristic of subpopulation of cells. The level of Lys-9 methylated FI3 in wild type cells and Suv39h double null cells was similar, demonstrating that other FI3 methyltransferases were involved [195]. Phosphorylation of Ser-10 by Ipll/aurora was also studied. Acetylation at Lys-14 promoted the activity of the mitotic kinase, while dimethylation, but not acetylation at Lys-9, reduced activity of the kinase [186]. 

Gregory, R.I. et al. (2002) Inhibition of histone deacetylases alters allelic chromatin conformation at the imprinted U2afl-rsl locus in mouse embryonic stem cells. J. Biol. Chem. 277, 11728-11734. Thomas, G., Lange, H.W., and Hempel, K. (1975) Kinetics of histone methylation in vivo and its relation to the cell cycle in Ehrlich ascites tumor cells. Eur. J. Biochem. 51, 609-615. 

Irradiated wild type (WT) and CD 154 expressing (CD 154) NIH3T3 mouse embryonic fibroblasts (see Note 2) WT and CD154 NIH3T3 cells are cultured in complete DMEM and maintained in a humidified atmosphere of 5% CO at 37°C. 

Sachinidis A, Eleischmann BK, Kolossov E, Wartenberg M, Sauer H, Hescheler J. Cardiac specific differentiation of mouse embryonic stem cells. Cardiovasc Res 2003 58 278-291. 

Jchibler Bert Van der Horst did an experiment where he showed that he couldn t induce circadian rhythms in Crj double knockout mouse embryonic fibroblasts. This is not surprising because they probably need the same components than in the clock. My strong prediction would be that everything is flat in the periphery. 

Agnish ND, Kochhar DM (1976) Direct exposure of mouse embryonic limb-buds to 5-bromodeoxyuridine in vitro and its effect on chondrogenesis increasing resistance to the analog at successive stages of development. J Embryol Exp Morphol 36(3) 639-652... 

Bain G, Ray WJ, Yao M et al (1996) Retinoic acid promotes neural and represses mesodermal gene expression in mouse embryonic stem cells in culture. Biochem Biophys Res Commun 223(3) 691-694... 

NHEERL J1 mouse embryonic stem cells, 9-day (mESC) ACDC assay for cell number and... 

Barrier M, Jeffay S, Nichols HP, Chandler KJ, Hoopes MR, Slentz-Kesler K, Himter ES 111 (2011) Mouse embryonic stem cell adherent cell differentiation and cytotoxicity (ACDC) assay. Reprod Toxicol 31 383-391... 

---