Cell line mouse embryonic fibroblast

Spielmann H, Pohl I, Doering B, Liebsch M, Moldenhauer E (1997) The embryonic stem cell test, an in vitro embryotoxicity test using two permanent mouse cell lines, 3 T3 fibroblasts and embryonic stem cells. In Vitro Toxicol 10 119-127... 

Another important discovery was made when comparing MVLBisG2 and DOTAP in a number of different cell lines. As shown in Fig. 12, complexes of MVLBisG2 efficiently transfect a variety of mouse and human cells in culture [24]. Their TE reaches or surpasses that of optimized complexes prepared from commercially available DOTAP. Most importantly, complexes containing MVLBisG2 are significantly more transfectant over the entire composition range in mouse embryonic fibroblasts (MEFs). MEFs are important as feeder cells for embryonic stem cells and are a cell line that is empirically known to be hard to transfect. 

Fig. 12 Transfection efficiencies for DOTAP/DOPC and MVLBisG2/DOPC complexes in four different cell lines, plotted against the mole fraction of cationic lipid. The data points were obtained at a constant pchg (7 for HeLa cells, 4.5 for all others), corresponding to a constant amount of DNA applied to the cells for each data point in a plot. Remarkably, MVLBisG2 complexes are significantly more transfectant in mouse embryonic fibroblasts, a cell line empirically know to be hard to transfect and of large practical importance as feeder cells for embryonic stem cells. Reprinted with permission from [24]. Copyright 2006 American Chemical Society...

Cells and cell lines NIH 3T3, mouse embryonic fibroblasts K562, human chronic myeloid leukemia MDA-MB-231, human breast cancer MiaPaCa2, human pancreatic cancer n/d, not determined NSCLC, nonsmall cell lung cancer p-, phosphporylated form of a protein RB, retinoblastoma protein. 

Cell lines Pluripotent hESC H9.2 clone [56], mouse embryonic fibroblast (MEF) feeder layer. 

Primary mouse embryonic fibroblast (EMFI) cells (37) or the STO fibroblast cell line (38) is the most commonly used feeder layers. Details for the preparation of a stock of EMFI or STO cells are described elsewhere (39). A brief protocol for the preparation of EMFI feeder layers will be given here. 

Qiu, H. Y, Y. Fujimori, S. R. Kai et al. 2003. Establishment of mouse embryonic fibroblast ceU lines that promote ex vivo expansion of human cord blood CD34(-l-) hematopoietic progenitors./ffemfltoffier Stem Cell Res 12(1) 39-46. 

Fetner has also demonstrated chromatid breaks in a human tissue-culture cell line exposed to ozone at 8 ppm for 5 min. Other tissue-culture studies include that of Sachsenmaier et who noted tetraploidy and other chromosomal abnormalities in embryonic chick fibroblasts exposed to ozone and a decrease in transplantability of mouse ascites tumor cells. In addition, Pace et demonstrated an interference by ozone with mitotic activity in two tissue-culture cell lines. More recently, Booher et al. reported that lung cells exposed in culture to ozone concentrations as low as 0.3 ppm demonstrated an inhibition in growth that was proportional to the ozone concentration. 

The EST has been developed with the aim to exploit the characteristics and differentiation potential of mouse embryonic stem cells (ES cells), established from the early embryo in 1981 [4, 5], ES cells are cultured in suspension to induce the formation of embryoid bodies, and afterwards they are transferred in 24-well dishes to allow attachment and differentiation in contracting cardiomyocytes. The toxicological endpoint is the inhibition of cardiac differentiation. In parallel a cytotoxicity test is performed on undifferentiated ES cells and a control somatic (fibroblast) cell line (3T3). The concentrations of testing chemicals that induce 50 % of differentiation inhibition (ID50) and 50 % cytotoxicity (IC50) in ES cells and 3T3 cells are inserted in a validated prediction model to classify the test chemical as non-embryotoxic, moderate, or strong embryotoxic [2, 6, 7], The validation of the method has been... 

The embryonic stem-cell test (EST). The test is composed of two procedures, a cytotoxicity test, which is conducted with the mouse embryonic stem (ES) cell line D3 and cells of the differentiated mouse fibroblast cell hne 3T3, and a differentiation assay using D3 cells (Spielmann et al, 1997 Genschow et al, 2(X)0). Three toxicological endpoints were identified to classify the embryotoxic potential of chemicals ... 

---