Mouse ascites cells

Thyroid transcription factor-1 TTF-I) 8C7C3/1 40-kD member of NKx2 family of homeodomain transcription factors in lung and thyroid Mouse ascites Cell Marque NA HIER... 

Kinetic studies of the rate of accumulation of 2-/im particles by mouse L cell fibroblasts or by mouse ascites cells showed that only a few minutes mixing time suffice for comparative rate studies. A more-or-less steady state is reached in 10-15 min. 

HDET, L.A. Mechanism of premature polypeptide termination in a mouse ascites cell-free system programmed by EMC REA. J. Virol. (1976), 18, 788-792. 

E. eoli Yeast Pea seeds Mouse ascites cells Pig liver Other... 

The paramyxoviruses, largely because of their profound cell-fusing activity, have served as an important model of membrane perturbation by viruses. During Sendai virus-mediated fusion of mouse ascites cells, Pasternak and Micklem (1973) detected loss of intracellular metabolites coincident with inhibition of their accumulation from the medium. This failure to maintain selective permeability did not occur at 0°C and was unaffected by cytochalasin B which inhibits fusion by the virus. Chick embryo fibroblasts infected with Newcastle disease virus were found to release cellular enzymes, such as lactate dehydrogenase, glutamic oxaloacetic transaminase, and lysosomal enzymes (Katzman and Wilson, 1974). These cells also became... 

Kinniburgh, A., McMullen, M. D., and Martin, T. E., 1979, Distribution of cytoplasmic poly(A) RNA sequences in free mRNP and polysomes of mouse ascites cells, J. Mol. Biol. 132 695. 

Table 3. Quantitative analysis of the reaction between absorbed anti-poly I poly C serum 929 and total RNA from mouse ascites cells...

Anti-poly A poly U antibodies also react with natural polynucleotides, including reovirus RNA, tRNA and ribosomal RNA from mammalian cells. Inhibition studies have shown that the poly A poly U absorbs all the antibodies reacting with RNA from mouse ascites cells. 

Anti-poly G poly C reacts with total RNA extracted from chicken cells, mouse ascites cells, hamster tumor, rabbit kidney, sheep liver and human tumor (Table 7). Since no precipitation occurs with tRNA extracted from the same cells, these reactions must occur with ribosomal RNA. Preincubation of the RNA (mouse ascites cells) with pancreatic RNAase abolishes the reaction completely. Secondary structure plays a role in this immunoreaction since heating the RNA with 1% formaldehyde for 10 min at 100°C, followed by rapid cooling, also abolishes precipitation with the antibody. Lack of activity of tRNA (leukemic chicken myeloblasts) is not modified by this treatment (Nahon-Merlin et al., 1971). 

Grassetti, D.R., and Murray, J.F. (December 1967) The effect of 2,2 -dithiodipyridine on thiols and oxi-dizable substrates of Ehrlich ascites cells and of normal mouse tissues. Biochem. Pharmacol. 16(12), 2387-2393. 

Fetner has also demonstrated chromatid breaks in a human tissue-culture cell line exposed to ozone at 8 ppm for 5 min. Other tissue-culture studies include that of Sachsenmaier et who noted tetraploidy and other chromosomal abnormalities in embryonic chick fibroblasts exposed to ozone and a decrease in transplantability of mouse ascites tumor cells. In addition, Pace et demonstrated an interference by ozone with mitotic activity in two tissue-culture cell lines. More recently, Booher et al. reported that lung cells exposed in culture to ozone concentrations as low as 0.3 ppm demonstrated an inhibition in growth that was proportional to the ozone concentration. 

CVA, Chromosomal aberrations, mouse ascites tumour cells in vivo + 340 gg/mouse Schoneich (1967)... 

Epistephanine Weakly active in vitro against HeLa cells not active in vivo (mouse) against Ehrlich ascites cells 360... 

Fangchinoline Active against HeLa cells in vitro, not active toward Ehrlich ascites cells in mouse 360... 

Gibb, L.E. Eddy, A.A. (1972). An electrogenic sodium pump as a possible factor leading to the concentration of amino acids by mouse ascites-tumour cells with reversed sodium ion concentration gradients. Biochem. J. 129, 979-981. 

Reid, M. Eddy, A.A. (1971). Apparent metabolic regulation of the coupling between the potassium ion gradient and methionine transport in mouse ascites-tumour cells. Biochem. J. 124,951-952. 

Cells in which chemiluminescence has been reported to originate include phagocytic cells, mouse spleen cells (Peterhans et al., 1980), rat thymocytes (Wrogemann et al., 1978), human NK cells (Roder et al., 1982), erythrocytes (canine) (Peerless and Stiehm, 1986), human epidermal cells (Fischer and Adams, 1985), Lettre ascites tumour cells (Mehta et al., 1985), colonic epithelial cells (Crawen et al., 1986) and Walker carcinosarcoma cells (Leroyer et al., 1987). 

Ascites Production. Anti-albumin clones HSA-1 and HSA-2 were thawed, subcloned, and reassayed by ELISA on human albumin, and a rapidly growing subclone was expanded for ascites production. Forty male BALB/c mice were primed with an intraperitoneal injection of 0.5 mL pristane on day 0. On day 14, 2 X 106 cells were injected into each mouse. Ascites fluid was collected over a 1-week period and pooled. Cells and other debris were removed by centrifugation. Approximately 180 mL of ascites fluid was obtained. 

Moser H Vecchio G (1967) The production of stable steady states in mouse ascites mast cell cultures maintained in the chemostat. Experientia 23 1-10. 

Fig. 4. In vivo gene delivery into mouse ascites tumor cells with Bubble liposomes. S-180 cells (1x10 cells) were i.p. injected into ddY mice. After 8 days, the mice were anaesthetized, then injected with 510 p.L of pCMV-Luc (10 p.g) and Bubble liposomes (500 pg) in PBS. Ultrasound (frequency 1 MHz, duty 50% intensity 1.0 W/cm, time 1 min) was transdermally applied to the abdominal area. In another experiment, pCMV-Luc (10 pg) - Lipofectin (50 pg) or Lipofectamine 2000 (50 pg) complex was suspended in PBS (510 pL) and injected into the peritoneal cavity of mice. After 2 days, S-180 cells were recovered from the abdomens of the mice. Luciferase activity was determined, as described in Materials and Methods. Each bar represents the mean S.D. (/j=3-6). P<0.01 compared to the group treated with plasmid DNA, Bubble liposomes, ultrasound exposure or lipofection with Lipofectin or Lipofectamine 2000. LF, Lipofectin. LF2000, Lipofectamine 2000. <10 BLU/mg protein...

The potential cytotoxic activities of forty-six Strychnos alkaloids, including harmine, were tested on different cancer or normal cells cultured in vitro. At a concentration of 1-10 pg/ml, harmine showed modest activity against L 1210 cells derived from DB A/2 mouse ascites tumor and cultured HeLa cells derived from human carcinoma. The alkaloid displayed only slight activity against cultured flow 2002 cells derived from normal embryonic human lungs, and was inactive against cultured B16 melanoma cells derived from C57BL mouse melanoma [273]. 

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