Leukemic chicken myeloblasts

Anti-poly G poly C reacts with total RNA extracted from chicken cells, mouse ascites cells, hamster tumor, rabbit kidney, sheep liver and human tumor (Table 7). Since no precipitation occurs with tRNA extracted from the same cells, these reactions must occur with ribosomal RNA. Preincubation of the RNA (mouse ascites cells) with pancreatic RNAase abolishes the reaction completely. Secondary structure plays a role in this immunoreaction since heating the RNA with 1% formaldehyde for 10 min at 100°C, followed by rapid cooling, also abolishes precipitation with the antibody. Lack of activity of tRNA (leukemic chicken myeloblasts) is not modified by this treatment (Nahon-Merlin et al., 1971). 

Although the anti-poly G poly C sera react with poly dG poly dC, no precipitation with Micrococcus lysodeikticus DNA (72% GC) is observed, whether native or heat-denatured DNA is used. Negative results are also obtained with native or denatured DNA from E, coli, Clostridium j>erfringens, phage 2 C, chicken myeloblastic leukemic cells or calf thymus. In contrast, antibodies to poly G poly C react with RNA and appear to be capable of distinguishing ribonucleic acids according to their origin. 

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