Cells Ehrlich ascites

L Na -iiidepeiideiit Braiiched-chaiii and aromatic amino acids Ehrlich ascites cells Chinese hamster ovary cells Hepatocytes... 

Hall IH, Liou YF, Lee KH. Antitumor agents LII The effects of molephantinin on nucleic acid and protein synthesis of Ehrlich ascites cells. J Pharm Sci 1982 71 687-690. 

Grassetti, D.R., and Murray, J.F. (December 1967) The effect of 2,2 -dithiodipyridine on thiols and oxi-dizable substrates of Ehrlich ascites cells and of normal mouse tissues. Biochem. Pharmacol. 16(12), 2387-2393. 

Chromatographic evidence has been obtained for the formation of 6-thioguanosine di- and triphosphates in Ehrlich ascites cells [197] and their formation is supported by the demonstrated incorporation of thioguanine into nucleic acids as thioguanylic acid [198]. The incorporation of both the a- and 3-anomers of 2 -deoxythioguanosine (XXXVI) [199] into DNA without... 

Cordycepin-1-oxide (XLVIIl) is activated in Ehrlich ascites cells by reduction to cordycepin [220] and a similar A -oxide reduction probably underlies the action of 6-mercaptopurine-3-oxide (XLIX) [221]. 

Fig. 2 a) Results of gel fraction assay on cytoplasmic extracts of Ehrlich ascites cells (after Ishiura and 0kada(13)). 

Hall, I. H., . H. Lee, W. L. Williams, T. Kimura, and T. Hiryama. 1980. Antitumor agents XLI effects of eupaformosanin on nucleic acid, protein, and anerobic and aerobic glycolytic metabolism of Ehrlich ascites cells. J. Pharm. Sci. 69 294-297. 

Inhibition of mainly DNA synthesis has been shown for Ehrlich ascites cells (43) and human HV3 cells (43a), as studied by the uptake of radiolabeled DNA, RNA, and protein precursors. However, RNA and protein inhibition are still being examined (44). 

The nine steps involved in the purification of UMP synthase from starting tissue are outlined in figure 6.8. All steps were carried out at 0-5°C. About 200 g of Ehrlich ascites cells, a mammalian tumor rich in the desired enzymes, was suspended in buffer and processed in a tissue homogenizer, which mechanically breaks down the tissue... 

Epistephanine Weakly active in vitro against HeLa cells not active in vivo (mouse) against Ehrlich ascites cells 360... 

Fangchinoline Active against HeLa cells in vitro, not active toward Ehrlich ascites cells in mouse 360... 

Active against HeLa-S3 cells in vitro ineffective in vivo toward Ehrlich ascites cells in mice 360... 

Ineffective in inhibiting mitochondrial respiration of Ehrlich ascites cell suspensions 458... 

Thalicberine Active against HeLa and Ehrlich ascites cells in 360... 

Colombini, M. Johnstone, R.M. (1974). Na+ gradient stimulated AIB transport in membrane vesicles from Ehrlich ascites cells. J. Membr. Biol. 18, 315-334. 

McCormick, J.I. Johnstone, R.M. (1988a). Simple and effective purification of a Na+-dependent amino acid transport system from Ehrlich ascites cell plasma membrane. Proc. Natl. Acad. Sri. USA 85,7877-7881. 

McCormick, J.I., Tsang, D Johnstone, R.M. (1984). A simple and efficient method for reconstitution of amino acid and glucose transport systems from Ehrlich ascites cells. Arch. Biochem. Biophys. 231,355-365. 

Viallet, P., Kohen, E., Schactschabel, D.0., Marty, A., Salmon, J.M., Kohen, C., Leising, H.B. and Thorell, B. The effect of atebrine and an acridine analog (BCMA) on the coenzyme fluorescence spectra of cultured melanoma and Ehrlich ascites cells (EL2 cells). Histochemistry 57,... 

Early mechanistic studies in the 1960s demonstrated that tylophorine alkaloids irreversibly inhibit DNA synthesis and affected protein synthesis at the elongation stage of the translation cycle [84], In HeLa cells, tylophorine (1) reversibly inhibits RNA synthesis and irreversibly inhibits DNA synthesis, but a predominant effect is exerted on protein synthesis and elongation of peptide ehains by preventing breakdown of polyribosomes and release of nascent peptides. Tyloerebrinc (2) was shown to have equivalent activity to emetine on inhibition of protein synthesis in Entamoeba histolytica [85]. Tylophorine and tylocrebrine were also found to inhibit protein synthesis in Ehrlich ascites cells and... 

Similar to the in vitro study using Lewis lung cells, it was observed that animals pretreated with pyran had no detectable Ehrlich ascites cells in phase GgM and only a few in the S phase after 2 or 6 days (Table XIV) (60) compared to pyran untreated mice which had 58 in the S phase and 22 in the GgM phase. Consequently, the use of pyran activated macrophage resulted in a) a high overall level of tumoricidal activity both in vivo and in vitro, b) the appearance of a tumor cell population with 50 of their normal DNA content and c) a shift of tumor cells from phase GgM to G. These results indicate that a possible mechanism of activated macrophage tumoricidal activity involves the induction of tumor cells with a reduced DNA content. 

TCII plays a major role in transport of cobalamins to tissues. A receptor for the TCII-B12 complex has been tentatively identified in vitro on HeLa cells,Ehrlich ascites cells,and human fibroblasts. The bound complex is transferred into the cell, where the cobalamin is released and the TCII is degraded in lysosomes (Figure 38-18). An inborn error of vitamin Bj2 metabolism has been attributed to a defect in vitamin B12 release from lysosomes. A congenital... 

In some 6-substituted purine 3 -deoxyribonucleosides,a direct correlation between the extent of phosphorylation of the nucleosides and inhibition of RNA synthesis in intact Ehrlich ascites cells has been shown. 

Refsnes, K., Olsnes, S. and Pihl, A. (1974) On the toxic proteins abrin and ricin. Studies of their binding to and entry into Ehrlich ascites cells. J Biol Chem, 249, 3557-3562. 

Lectin reactivities of Ehrlich ascites cell variants ... 

---