Microorganisms culture collections

A seed culture of S. cerevisiae ATCC 24860 (American Type Culture Collection, Manassas, VA, USA) was grown in a media of 5g glucose, and 0.5 g yeast extract, respectively, 1.5 g KH2P04 and 2.25 g Na2P04 phosphate buffer up to a total volume of distilled water, 500 ml. The media was sterilised at 121 °C for 15 min. The stock culture of the microorganisms was transferred to the broth media for preparation of seed culture. 

The reproducibility of a determination is a critical element of a standard method, so they are carefully written to ensure that they can be followed accurately by any qualified laboratory. The designated test biological RMs must not only be reproducible in their reactions to the performance tests, but they must also respond in a predicable manner. In order to guarantee that the same strain of microorganisms could always be available, many of these biological RMs have been deposited with the ATCC and other culture collections around the world. 

China Committee of Culture Collection for Microorganisms (CCCCM). 

Institute for Fermentation, Osaka (IFO) Culture Collection of Microorganisms (In Japanese). 

KUKENS collection, Istanbul Faculty of Medicine, Centre for Research and Application of Culture Collections of Microorganisms an introduction. 

National Culture Collection of Armenia for Microorganisms (RCDM) an introduction. 

World Data Centre for Microorganisms - Microbial Collections - WDCM Statistics The Culture Collections in the world. 

The bacterial strains were submitted to the National Collection of Microorganism Cultures of the Pasteur Institute (CNCM) and included R. erythropolis CNCM 1-2204, -2205, -2207, and -2208, and/or R. rhodnii 1-2206. The activity of the microorganisms includes a selective attack on the carbon-sulfur bonds in the organic molecules without significant alteration of the carbon atom structure. As usual, strains were isolated and subjected to successive enrichment phases, in media containing various sources of carbon and DBT as the only sulfur source, then a purification of the enhanced culture led to the fifteen DBT specific strains. From those strains, a subset of ten strains was selected... 

Biodegradation studies may use either pure or mixed cultures of microorganisms. Pure cultures can be obtained either from culture collections or by enrichment from natural samples (sewage, soil, river water, etc.). In the latter case, cultures usually are isolated by serial transfer in media containing the test chemical as the sole carbon source. 

To locate sources of cloned materials, cell lines, or cultures of live microorganisms American Type Culture Collection... 

When a suitable biocatalyst for a desired biotransformation is not always available it becomes necessary to screen for a new or improved biocatalyst. Traditionally, this has been done by screening living microorganisms from either environmental samples or culture collections. With the recent advances in molecular biology and the explosion of genomic sequences it is possible to isolate... 

Because the specific microorganism cultured is the most critical factor in any antibiotic process, strain identification, including morphologic, cultural, and biochemical characteristics should be performed. The source of the microbial isolate (e.g., soil, air, water), as well as any genetic engineering or mutation procedures should be documented. Microbial deposition should be reported (e.g., American Type Culture Collection or Type Culture Collection of the U.S. Department of Agriculture). 

As of 10 December 2008, 544 culture collections in 68 countries had been registered at WDCM (World Data Centre of Microorganisms http // wdcm.nig.ac.jp/hpcc.html) containing 1 413 100 microbial cultures. 

Microbial Sources. In many cases, screening for the microbial production of volatiles can be achieved by selecting pure cultures of microorganisms from public collections such as the American Type Culture Collection (ATCC). The chances of obtaining an effective culture can often be improved by selecting organisms known to perform the desired types of reactions. 

A partial listing including web addresses of some of the largest culture sources are given in Table 2, and many of these have links to most of the other available strain collections. Some of these include the American Type Culture Collection (ATCC) and the DSMZ German Collection of Microorganisms and Cell Cultures. 

Blood cultures usually should be performed in the acutely ill febrile patient. Blood culture collection should coincide with sharp elevations in temperature, suggesting the possibility of microorganisms or microbial antigens in the bloodstream. Ideally, blood should be obtained from peripheral sites as two sets (one set consists of an aerobic bottle and one set an anaerobic bottle) from two different sites approximately 1 hour apart. In selected infections, bacteremia is qualitatively continuous (e.g., endocarditis), so cultures may be obtained at any time. ... 

There are far more microorganisms available from culture collections than enzymes on the market. A list of important culture collections can be found in Table 1-6. Further information can be found on the internet http //wdcm.nig.ac. jp/wfcc/wfcc.html... 

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