German Collection of Microorganisms and Cell Culture

German Collection of Microorganisms and Cell Cultures Mascheroder Weg 1 b, Braunschweig, Germany DSM... 

A partial listing including web addresses of some of the largest culture sources are given in Table 2, and many of these have links to most of the other available strain collections. Some of these include the American Type Culture Collection (ATCC) and the DSMZ German Collection of Microorganisms and Cell Cultures. 

The strain used in this study was Thermoanaerobacter BGILI, which is a lactate dehydrogenase-defieient mutant of the thermophilic anaerobic bacterial strain BGl. The strains Thermoanaerobacter BGl and BGILI have been deposited in the German Collection of Microorganisms and Cell Cultures as patented strains. 

Inoculum was prepared by growing cells in 100-ml vials containing 45 ml anaerobic synthetic medium (BA medium) [13], amended with 1 g/1 yeast extract but without cystein. The medium was neutralized and flushed for 15 min with a mixture of N2/CO2 (4 1) to ensme anaerobic conditions before autoclaving at 140°C for 20 min. Prior to inoculation, the mediinn was reduced with a sterile anaerobic solution of sodium sulfide to a final concentration of 0.5 g/1. Xylose and vitamins were further added from filter-sterilized anaerobic solutions of D-xylose and vitamins DSMZ medium No 141 (German Collection of Microorganisms and Cell Cultures) to initial concentrations of 5 g/1 and 10 ml/1, respectively. The medium was inoculated with 10% (v/v) culture and incubated overnight at 70°C in the dark without shaking. 

DSMZ German Collection of Microorg. and Cell Cultures Microbial Information Network of China MSDN Microbial Strain Data Network World Data Center for Microorganisms (Japan)... 

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