Broth media

A seed culture of S. cerevisiae ATCC 24860 (American Type Culture Collection, Manassas, VA, USA) was grown in a media of 5g glucose, and 0.5 g yeast extract, respectively, 1.5 g KH2P04 and 2.25 g Na2P04 phosphate buffer up to a total volume of distilled water, 500 ml. The media was sterilised at 121 °C for 15 min. The stock culture of the microorganisms was transferred to the broth media for preparation of seed culture. 

J. Carlsson et al., Hydrogen peroxide and superoxide radical formation in anaerobic broth media exposed to atmospheric oxygen. Appl. Environ. Microbiol. 36, 223-229 (1978)... 

Quality control of each batch of complete broth media should be performed before use by adding at least two different strains of positive control myco-plasmas. A non-inoculated broth should be incubated as a negative control. 

Broth media Dissolve 2 g of mycoplasma broth base in 70 mL distilled water, and autoclave at 15 lb/in.2 for 15 min. 

Figure 4.22 The slow release of Mgp2 NPs inn the broth media and the high killing effect on the planktonic population.

Dilution susceptibility testing methods are used to determine the minimal concentration of antimicrobial required to inhibit or kill the microorganism. This can be achieved by dilution of an antimicrobial in either agar or broth media [20]. 

These tests can be carried out individually, for example, in broth media containing the specifically required nutrients and/or reagents, but they are more commonly performed using commercial kits or automated systems which have the potential to give a rapid identification based on biochemical profiles. 

Use 1-3 mL of the above overnight culture to inoculate 200-mL to 1 L of LB broth media with appropriate antibiotic amounts and grow at 37 °C for 3-5 h. Draw 1 mL cultures to measure the OD at 600 nm between these time periods. 

Figure 5.20 Weight loss study of in-situ and ex-situ prepared PMMA/ceUulose nanocomposite films in liquid broth media inoculated with pure culture. Reproduced from [69] with permission of Elsevie.

The incubation temperature used to culture Aliivibrio sabnonicida is an important aspect of vaccine production, with 10 C (this coincides with the upper range of water temperatures at which the disease coldwater vibriosis is most likely to occur) rather than 15 C giving a higher yield of cells in broth media (Colquhoun et al, 2002). 

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