Methylcholanthrene-induced

Recently, the mechanism of 6-nitro-BaP ring hydroxylation has been elucidated by using 3-deutero-6-nitro-BaP (144). When incubated with 3-methylcholanthrene-induced rat liver microsomes, this deuterated analogue yielded the same metabolite profile previously observed with 6-nitro-BaP. Spectroscopic analysis of 3-hydroxy-6-nitro-BaP and 6-nitro-BaP-3,9-hydroquinone indicated that 30% of the deuterium label had migrated to carbon 2, presumably via an NIH shift. Therefore, it appears that 6-nitro-BaP-2,3-oxide is a common intermediate for these two metabolites. 

Bock KW, Schirmer G, Green MD, et al. 1988. Properties of 3-methylcholanthrene-inducible phenol UDP-glucuronosyltransferase from rat liver. Biochem Pharmacol 37 1439-1443. 

Kouri RE, Ratrie H, Whitmire CE. 1974. Genetic control of susceptibility to 3-methylcholanthrene-induced lung subcutaneous sarcomas. J Cancer 13 714-720. 

Nebert. Isolation and characterization of a cloned DNA sequence associated with the murine Ah locus and a 3-methylcholanthrene-induced form of cytochrome P-450. Proc. Natl. Acad. Sci. U.S.A. 78 800-804, 1981. 

Hussain, S.P. and Rao, A.R. (1991) Chemopreventive action of mace (Myristica fragrans Houtt) on methylcholanthrene-induced carcinogenesis in the uterine cervix in mice. Cancer Letters 56(3), 231-234. 

Knockout systems ethylnitrosourea induced uterine tumors in p53 knockout mice. susceptible to methylcholanthrene-induced tumorigenesis. [113] well studied. Can be created for most cytokines or effector cells. May provide strong proof of concept. genes. Compensatory effects may mask deficit. All or nothing effect may overestimate hazard. 

Huang DY, Ohnishi T, Jiang H, Furukawa A, Ichikawa Y. 1999. Inhibition by retinoids of benzo(A)pyrene metabolism catalyzed by 3-methylcholanthrene-induced rat cytochrome P-450 1A1. Metabolism 48 689-92... 

Aloctin A is a glycoprotein obtained from A. aborescens Miller. Aloctin A administered to six mice at a dose of 10 mg/kg/d intraperitoneally for 5 d inhibited growth of methylcholanthrene-induced fibrosarcoma in four of the mice vs zero of ten untreated mice. These results were attributed to aloctin A s immunomodulatory effect, not cytotoxicity (Imanishi et al., 1981). 

Imanishi K, Ishiguro T, Saito H, Suzuki I. Pharmacological studies of plant lectin, Aloctin A. I. Growth inhibition of mouse methylcholanthrene-induced fibrosarcoma (Meth A) in ascites form by Aloctin A. Experientia 1981 37 1186-7. 

Masuda Y, Yasoshima M. 1988. Loss of 3-methylcholanthrene-inducible form of cytochrome P-450 in liver microsomes following administration of carbon disulfide in C57BL/6 Cr mice. Biochem Pharmacol 37 2363-2371. 

Yasoshima M, Masuda Y. 1988. Effects of carbon disulfide on liver microsomal cytochrome p-450 in phenobarbitol and 3-methylcholanthrene-induced animals. Jpn J Pharmacol 46 ... 

Scuderi P, Rosse C. 1981b. The role of bone marrow cells in the growth inhibition of trasplanted methylcholanthrene-induced sarcoma (MCA). XIII(l) 747-751. 

Luukkanen L, Elovaara E, Lautala P, et al. 1997. Characterization of 1-hydroxypyrene as a novel marker substrate of 3-methylcholanthrene-inducible phenol UDP-glucutonosyltransferase(s). Pharmacol Toxicol 80 152-158. 

Ebel, S. Liedtke, R. Missler, B. [Quantitative determination of nitrofurantoin in body fluids by direct injection HPLC]. Arch.Pharm.(Weinheim)., 1980, 313, 95-96 Jonen, H.G. Oesch, F. Platt, K.L. 4-Hydroxylation of nitrofurantoin in the rat. A 3-methylcholanthrene-inducible pathway of a relatively nontoxic compound. Drug Metab.Dispos., 1980, 8, 446-451... 

Methylcholanthrene- induced Adenovirus type 12-induced 1x10 10x3 Tumor occurence 83 - 33 % 79 - 40 % ... 

Comparison of the metabolic profiles of benzo(a)pyrene obtained from primary cell cultures and subcellular fractions derived from normal and methylcholanthrene-induced rat liver. Cancer Letters 5 81-89. 

The rate of chromate reduction using NADH as a cofactor did not vary with the source of microsomes. The same kinetic parameters were found with control, phenobar-bital-induced and 3-methylcholanthrene-induced microsomes. Therefore there was no correlation of rate of chromate reduction with the activity of NADH-Cytochrome P-450 reductase in the microsomes which was 1.25 times greater in the control set than in either the phenobarbital- or 3-methylcholanthrene-induced microsomes. The cytochrome bs content of the microsomes, however, did not vary ( 5%) with the source of microsomes. 

Suga, T., Shiio, T., Maeda, Y., and Chihara, G., Antitumor activity of Lentinen in murine syngenic and autochthonous hosts and its suppressive effect on 3-methylcholanthrene-induced carcinogenesis, Cancer Res., 44, 5132-5137,1984. 

Methylcholanthrene-induced (Meth-A) mouse tumor cells grown in an astaxanthin-supplemented medium had reduced cell numbers and lower DNA synthesis rates 1 to 2 days postincubation than control cultures. Similarly, astaxanthin inhibited murine... 

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