Methylated serum albumin

The lipids are classical substances to be complexed for immunization with heterologous proteins, formerly called Schlepper proteins. On the other hand, nucleic acids and nucleotides have only recently been jfound to be immunogenic, provided these polyacidic derivatives are combined with polybasic carriers like methylated serum albumin. 

Plescia OJ, Braun W, Imperato S, Cora-Block E, Jaroskova L, Schimbor C (1968) Methylated serum albumin as a carrier for oligo- and polynucleotides. In Plescia OJ, Braun W (eds) Nuclei acids in immunology. Springer, New York Pressmann D, Goldberg AL (1968) The structural basis of antibody specificity. Benjamin, New York... 

When this synthetic polyribonucleotide complex, poly I poly C adsorbed onto methylated serum albumin is used as immunogen, antibodies reacting... 

This experiment provides a nice example of the application of spectroscopy to biochemistry. After presenting the basic theory for the spectroscopic treatment of protein-ligand interactions, a procedure for characterizing the binding of methyl orange to bovine serum albumin is described. 

Data from a single study in dogs suggest that hepatic first-pass metabolism may limit systemic availability of the parent compound following oral exposure (Braeckman et al. 1983). Placental transfer of methyl parathion was demonstrated in pregnant rats 1-3 days before parturition. Thirty minutes after administration, both methyl parathion and methyl paraoxon were found in fetal brain, liver, and muscle methyl parathion, but not methyl paraoxon, was detected in placenta and maternal liver (Ackermann and Engst 1970). Methyl parathion binds reversibly to serum albumin, but is readily distributed to the tissues (Braeckman et al. 1980, 1983). 

GME glycine methyl ester GDM glutamic dimethyl ester EDA ethylendiamine GAM glucosamine HDA hexadecylamine BSA bovine serum albumin OVA ovalbumin CA carbonic anhydrase MYO myoglobin H alkaline hydrolysis for converting ester groups from GME or GDM into free carboxylic acid groups that were subsequently activated with EDC for further modifications. 

MARCKS Myristolated, alanine-rich C kinase substrate specific protein kinase C substrate MBP Major basic protein MBSA Methylated bovine serum albumin... 

Pandurangi, R.S. et al. (1995b) High efficiency photolabeling of human serum albumin and human g-globulin with [14C]Methyl 4-azido-2,3,5,6-tetrafluorobenzoate. Bioconjugate Chem. 6, 630-634. 

Recent studies with quinoline-4-carboxylic acid angiotensin II receptor antagonists have confirmed the interest of (oxodioxolyl)methyl esters as prodrugs with improved oral bioavailability and efficacy in laboratory animals [79], Olmesartan, another angiotensin II receptor antagonist, has also been derivatized to a (5-methyl-2-oxo-l,3-dioxol-4-yl)methyl carboxylate designated as olmesartan medoxomil [80], In this case, both human serum albumin and arylesterase (presumably EC 3.1.1.2, although paraoxonase cannot be excluded) were shown to be involved in hydrolysis. 

Fig. 4. Extent of binding of methyl orange at pH 7.0 and 25°C as a function of free (nonbound) dye concentrations (1) polyethylenimine with 8.4% of residues acylated by lauroyl groups (2) polyethylenimine with 11.5% of residues acylated by hexanoyl groups (3) polyethylenimine with 10% of residues acylated by butyrl (O) or isobutyrl ( ) groups (4) Polyethylenimine, PEI-600 (5) bovine serum albumin.

The enantioselective oxidation of prochiral sulfides with DMD has been achieved by using bovine serum albumin (BSA) as the chiral inductor Moderate to good enan-tioselectivities have been reported in the presence of this protein, for which a typical example is shown in equation 22 . As yet, however, no enantioselective oxidation of a prochiral sulfide has been documented by employing an optically active dioxirane. We have tried the enantioselective oxidation of methyl phenyl sulfide with the dioxirane generated from the ketone 7 (Shi s ketone), but an ee value of only ca 5% was obtained. One major hurdle that needs to be overcome with such enantioselective dioxirane oxidations is the suppression of the background oxidation of the sulfide substrate by Caroate, an unavoidable feature of the in-situ mode. 

Fig. 12.1.4 Immobilization of bovine serum albumin on colloidal silica grafted with poly (maleic anhydride-styrene) (O), poly(maleic anhydride-methyl methacrylate) ( ), and poly (ethylene glycol)-poly(maleic anhydride-styrene) (A). (Front Ref. 42.)...

Fig. 1. Comparison of binding affinities of bovine serum albumin (BSA), polyvinylpyrrolidone (PVP), and polylysine (POLYLYS) for methyl orange anions (A), in acetate buffer, pH 5.6, 0.1 ionic strength, and 25°C.

Zhang, F., Guo, J., Peng, X., Fi, Y. (2004) Preparation and release behaviour of carboxy-methylated chitosan/alginate microspheres encapsulating bovine serum albumin. Journal of Applied Polymer Science, 92, 878-882. 

Interpretation of the NMR Spectra of Membranes. Let us first consider a model system of lysolecithin and serum albumin (74), shown in Figure 12. Lysolecithin in D20 is shown in Figure 12A, while the effect of increasing amounts of bovine serum albumin is shown in Figures 12B and 12C. When no protein is present, the lines for both the methylene protons on the lysolipid tails and the quaternary ammonium methyl protons on the choline moiety of the polar heads are narrow. Thus, the polar ends of the molecules are free to move, and the apolar hydrocarbons within the detergent micelles are in a liquid-like state (24). When protein is added, the hydrocarbon proton line broadens and shifts upfield slightly, but neither the width nor area of the quaternary ammonium line... 

NBD-amino acids (serine, threonine, valanie, phenylalanine, and histidine) were separated by using a 20 mM ammonium acetate buffer (pH 9.0) containing 10 mM copper acetate, 20 mM L-prolinamide, and 1 mM SDS. The elution order was the L-enantiomer followed by the D-form except in NBD-histidine. Weng et al. [32] described chiral separation of tryptophan on a poly(methyl methacrylate) microfluidic chip coated with bovine serum albumin (32 mm... 

Hpba Hppbpy Hppy Hpq HSA IgG IL LLCT mcsubpy 4-(2-Pyridyl)benzaldehyde 4-(4-Hydroxyphenyl)-6-phenyl-2,2 -bipyridine 2-Phenylpyridine 2-Phenylquinoline Human serum albumin Immunoglobulin G Intra-ligand Ligand-to-ligand charge transfer 4-Carboxy-4 -methyl-2,2 -bipyridine N-hydroxysuc- cinimide ester... 

Anti-a-D-mannose antibodies have been isolated from the serum of rabbits immunized with the glycoconjugate of a-D-mannose and bovine serum albumin [85], The antibodies were purified by affinity chromatography with adsorption on a mannosyl-Sepharose column and elution with a-D-mannose or methyl a-D-mannoside. Such antibodies should be especially useful for studying the detection of diseases due to the appearance of abnormal glycoproteins that contain mannose polymers. 

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