Methylated bovine serum albumin

MARCKS Myristolated, alanine-rich C kinase substrate specific protein kinase C substrate MBP Major basic protein MBSA Methylated bovine serum albumin... 

Methylated bovine serum albumin (mBSA) (from Sigma) Dissolve in water (1 mg/mL) and store at-20°C. 

Generally, all soluble immunogens or protein-hapten conjugates are mixed with, emulsified in or adsorbed onto adjuvants. The most widespread adjuvants are (i) Freund s adjuvants (ii) aluminum salts and bentonite (iii) methylated bovine serum albumin and, (iv) heat-inactivated Bordetella pertussis. 

Methylated bovine serum albumin (MBSA) MBSA... 

Water-in-oil emulsions such as Freund s complete adjuvant, Freund s incomplete adjuvant. The complete adjuvant contains QdX- aDlQ6. Mycobacterium tuberculosis bacteria whereas the incomplete does not. Both have the basis of an immune modulator, e.g., branched glucose polymers or methylated bovine serum albumin (Pierce s AdjuPrimeTM). 

The modified DNA (0.25-0.5mg) was electrostatically complexed to equal amounts of methylated bovine serum albumin in phosphate buffered saline (PBS), and emulsified with complete Freund s adjuvant. Immunization of rabbits and purification of IgG fractions were performed as described previously (6,7). 

MBSA=methylated bovine serum albumin CFA=complete Freund s adjuvant DH = delayed hypersensititivy Ab=antibody response RB=rabbits GP=guinea pigs H = humans IFA=incomplete Freund s adjuvant... 

The precipitate results ftom electrostatic motecular a regation of methylated bovine serum albumin (which has an overall positive charge) with DNA (polyanionk). 

Abbreviations DNA deoxyribonucleic acid RNA ribonucleic acid rRNA ribosomal RNA tRNA transfer RNA. A adenine U uracil G guanine C cytosine, poly A polyadenylic acid poly U polyuridylic acid poly C polycytidylic acid poly I poly-inosinic acid poly G polyguanylic acid. The letters r and d refer to the ribose or deoxy-ribose series. Complexes between the polynucleotides are written as follows polyG poly C double-stranded helical complex of poly G and poly C 2 poly G poly C, triple-stranded complex. BSA bovine serum albumin MBSA methylated bovine serum albumin. N nitrogen. AMV Avian myeloblastosis virus NDV Newcastle disease virus. SLE systemic lupus erythematosus. 

Plescia, O. J., Braun, W., Imperato, S., Cora-Block, E., Jaroskova, L., Schimbor, C. Methylated bovine serum albumin as a carrier for oligo and polynucleotides. In Nucleic acids in immunology, ed. by Plescia O. J. and Braun W. p. 5-17. Berlin-Heidelberg-New York Springer 1968. 

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