Med proteins

Urokinase (from human urine) [9039-53-6] Mr 53,000, [EC 3.4.21.31]. Crystn of this enzyme is induced at pH 5.0 to 5.3 (4") by careful addition of NaCl with gentle stirring until the soln becomes turbid (silky sheen). The NaCl concentration is increased gradually (over several days) until 98% of saturation is achieved whereby the urokinase crystallises as colourless thin brittle plates. It can be similarly recrystd to maximum specific activity [104K CTA units/mg of protein (Sherry et al. J Lab Clin Med 64 145 1964)]. [Lesuk et al. Science 147 880 1965 NMR Bogusky et al. Biochemistry 28 6728 1989.] It is a plasminogen activator [Gold et al. Biochem J 262 1989 ]. 

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Salo, D.C., Donovan, C.M., Davies, K.J, (1991). Hsp70 and other possible heat shock or oxidative stress proteins are induced in skeletal muscle, heart, and liver during exercise. Free Radic. Biol. Med 11,239-246. 

Smiley, J.D. Hoffman, W.L. (1991). The role of infections in the rheumatic diseases molecular mimicry between bacterial and human stress proteins Am. J. Med. Sci. 301, 138-149. 

Vanbuskirk, A., Crump, B.L., Margoliash, E., Pierce, S.K. (1989). A peptide binding protein having a role in antigen presentation is a member ofthehsp70 heat shock family.). Exp. Med. 170,1799-1809. 

Cinatl J Jr, Michaelis M, Doerr HW (2007b) The threat of avian influenza A (H5N1). IV. Development of vaccines. Med Microbiol Immunol 196 213-225 Colman PM (1994) Influenza virus neuraminidase structure, antibodies, and inhibitors. Protein Sd 3 1687-1696... 

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Townsend L, Devivar R, Turk S, Nassiri M, Drach J (1995) Design, synthesis, and antiviral activity of certain 2,5,6-trihalo-l-(beta-d-ribofuranosyl)benzimidazoles. J Med Chem 38 4098 105 Turlure F, Devroe E, Silver PA, Engelman A (2004) Human cell proteins and human immunodeficiency virus DNA integration. Front Biosd 9 3187-3208 Umehara T, Fukuda K, Nishikawa F, Kohara M, Hasegawa T, NisUkawa S (2005) Rational design of dual-functional aptamers that inhibit the protease and helicase activities of HCV NS3. J Biochem 137 339-347... 

Tyagi SR, Singh Y, Sriram K, et al. 1985. Quality and quantity of dietary protein and acute endosulfan metabolic toxicity in rat liver microsomes. Indian J Med Res 81 480-487. 

W. H. Discovery of nanomolar ligands for 7-transmembrane G-protein-coupled receptors from a diverse N-(substi-tuted)glycine peptoid library. J. Med. Chem. 1994, 37, 2678-2685. 

Romani N, Koide S, Crowley M. Witmer-Pack M. Livingstone AM, Fathman CG. Inaba K. Steinman RM Presentation of exogenous protein antigens by dendritic cells to T cell clones. Intact protein is presented best by immature, epidermal Langerhans cells. J Exp Med 1989 169 1169-1178. 18... 

Greer J, Erickson JW, Baldwin, JJ, Varney MO. Application of three-dimensional structures of protein target molecules in structure-based drug design. J Med Chem 1994 37 1035-54. 

Ferrara P, Gohlke H, Price DJ, Klebe G, Brooks CL. Assessing scoring functions for protein-ligand interactions. J Med Chem 2004 47 3032-47. 

Raha K, Merz KM. Large-scale validation of a quantum mechanics based scoring function predicting the binding affinity and the binding mode of a diverse set of protein-ligand complexes. J Med Chem 2005 48 4558-75. 

Roche O, Kiyama R, Brooks CL 3rd. Ligand-protein database linking protein-ligand complex strnctnres to binding data. J Med Chem 2001 44 3592-8. 

Bondensgaard K, Ankersen M, Thogersen H, Hansen BS, Wulff BS, Bywater RP. Recognition of privileged structures by G-protein coupled receptors. J Med Chem 2004 47 888-99. 

Erickson JA, Jalaie M, Robertson DH, Lewis RA, Vieth M. Lessons in molecn-lar recognition the effects of ligand and protein flexibility on molecular docking accuracy. J Med Chem 2004 47 45-55. 

Muegge I, Martin YC. A general and fast scoring function for protein-ligand interactions a simplified potential approach. J Med Chem 1999 42 791-804. 

Bissantz C, Folkers G, Rognan D. Protein-based virtual screening of chemical databases. 1. Evaluation of different docking/scoring combinations. J Med Chem 2000 43 4759-67. 

Charifson PS, Corkery JJ, Murcko MA, Walters,WP. Consensus scoring a method for obtaining improved hit rates from docking databases of three-dimensional structures into proteins. J Med Chem 1999 42 5100-9. 

Lavrador K, Murphy B, Saunders J, Struthers S, Wang X, Williams J. A screening library for peptide activated G-protein coupled receptors. 1. The test set. J Med Ghent 2004 47 6864-74. 

Forino M, Jung D, Easton JB, Houghton PJ, Pellecchia M. Virtual docking approaches to protein kinase B inhibition. J Med Chem 2005 48 2278-81. 

Vangrevelinghe E, Zimmermann K, Schoepfer J, Portmann R, Fabbro D, Furet P. Discovery of a potent and selective protein kinase CK2 inhibitor by high-throughput docking. J Med Chem 2003 46 2656-62. 

Doman TN, McGovern SL, Witherbee BJ, Kasten TP, Kurumbail R, Stallings WC, Connolly DT, Shoichet BK. Molecular docking and high throughput screening for novel inhibitors of protein tyrosine phosphatase IB. J Med Chem 2002 45 2213-21. 

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