Matrix-assisted laser desorption/ionization tandem time of flight

W. Wuhrer, C. H. Hokke, and A. M. Deelder, Glycopeptide analysis by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry reveals novel features of horseradish peroxidase glycosylation, Rapid Commun. Mass Spectrom., 18 (2004) 1741-1748. 

Macht, M., Asperger, A., and Deininger, S. O. (2004) Comparison of laser-induced dissociation and high-energy collision-induced dissociation using matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOE/TOF) for peptide and protein identification. Rapid Commun. Mass Spectrom. 18, 2093-2105. 

Conrotto, R Heilman, U. Lys Tag An easy and robust chemical modification for improved de novo sequencing with a matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometer. Rapid Commun. Mass. Spectrom. 2008, 22,... 

This chapter describes a step-by-step protocol for top-down proteomic identification of protein biomarkers of bacterial food-borne pathogens using matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF-TOF-MS/MS) and web-based software developed at the Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture (USDA). 

Alley, W. R., Jr., Mechref, Y., Klouckova, L, Novotny, M. V. (2007) Improved collision-induced dissociation analysis of peptides by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry through 3-sulfobenzoic acid succinimidyl ester labeling. / Proteome Res, 6, 124-132. 

Bennett, K.L., Stensballe, A., Podtelejnikov, A.V., Moniatte, M. and Jenson, O.N. (2002) Phos-phopeptide detection and sequencing by matrix-assisted laser desorption/ionization quadrupole time-of-flight tandem mass spectrometry. Journal of Mass Spectrometry 37, 1 79-1 90. 

Tandem mass spectrometry (MS/MS) is a method for obtaining sequence and structural information by measurement of the mass-to-charge ratios of ionized molecules before and after dissociation reactions within a mass spectrometer which consists essentially of two mass spectrometers in tandem. In the first step, precursor ions are selected for further fragmentation by energy impact and interaction with a collision gas. The generated product ions can be analyzed by a second scan step. MS/MS measurements of peptides can be performed using electrospray or matrix-assisted laser desorption/ionization in combination with triple quadruple, ion trap, quadrupole-TOF (time-of-flight), TOF-TOF or ion cyclotron resonance MS. Tandem... 

Loboda A.V., Krutchinsky A.N., Bromisrski M., Ens W., and Standing K.G. (2000), A tandem quadrupole/time of flight mass spectrometer with a matrix-assisted laser desorption/ionization source design and performance, Rapid Commun. Mass Spectrom. 14, 1047-1057. 

Yau, P.M. and Burlingame, A.L. (2002) Identification of acetylation and methylation sites of histone H3 from chicken erythrocytes by high-accuracy matrix-assisted laser desorption ionization-time-of-flight, matrix-assisted laser desorption ionization-postsource decay, and nanoelectrospray ionization tandem mass spectrometry. Analytical Biochemistry, 306, 259-269. 

Mechref Y, Kang P, Novotny M (2006) Differentiating structural isomers of sialylated gly-cans by matrix-assisted laser desorption/ionization time-of-flight/time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom 20 1381-1389. doi 10.1002/rcm.2445... 

High-Performance Liquid Chromatography LC/Electrospray Tandem MS 50 % Lethal Dose Matrix-assisted Laser Desorption Ionization/Time-Of-Flight/Mass Spectrometry propylsulfonic acid... 

Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is a direct mass spectral technique that has found application for the analysis of various phytochemicals. MALDI represents the ion source, whereas TOF is the mass analyzer. The two techniques may be coupled with other ion sources or mass analyzers, but are most commonly used in tandem. In MALDI, the analytes are mixed with a matrix, which is usually an aromatic organic acid, which aids in... 

The methods for each study are divided into the initial protein separation step, a second separation step if applicable, the type of mass analysis, and the software used for peptide identification. ID = one dimensional polyacrylamide gel electrophoresis, 2D = two dimensional polyacrylamide gel electrophoresis, MS = mass spectrometry (peptide mass fingerprinting), MS/MS = tandem mass spectrometry, MALDI-TOF = matrix assisted laser desorption/ionization-time of flight, MS FIT = software from Protein Prospector, http //prospector.ucsf edu/, ESI = electrospray ionization, Q-TOF = quadrupole-time of flight, PPSS2 =Protana s Proteomic Software Suite (ProtanaEngineering, Odense, Denmark), Mascot = Matiix Science, http //www.matrixscience.com/, TOF-TOF = MALDI plus TOF tandem mass spectrometry, RP-HPLC = reverse phase high performance liquid chromatography, Q-IT = quadrupole ion trap, LIT = linear ion trap. Bioworks = Thermo Electron Corporation. 

These interface techniques for connecting the HPLC with the MS-system are very sensitive for most of the substances of interest to the flavour industry. Therefore, HPLC-MS coupling techniques have become an increasingly powerful tool for quality control of flavourings, especially for the analysis of complex mixtures like process flavourings or contaminants present in such complex mixtures. New developments in the area of mass detection systems, such as time-of-flight (ToF) mass analysers and tandem mass spectrometry systems or the features of matrix-assisted laser desorption ionization (MALDI) techniques, may enhance the analytical capabilities of these systems in the near future [16, 17, 28-31 ]. 

Chen, R., Tseng, A. M., Uhing, M., and Li, L. Application of an integrated matrix-assisted laser desorption/ionization time-of-flight, electrospray ionization mass spectrometry and tandem mass spectrometry approach to characterizing complex polyol mixtures.. Am. Soc. Mass Spectrom., 12, 55, 2001. 

IM has been coupled with a range of mass spectrometers. Both electrospray ionization (ESI) [21,22], and matrix-assisted laser desorption/ionization (MALDI) [18] ionization sources have been used to generate ions prior to IM analysis. Different configurations of mass analyzers have been used to perform mass analysis including quadrupole mass analyzers [23], time-of-flight (TOE) analyzers [24] and Fourier transform ion cyclotron resonance spectrometry (FT-ICR) [25]. Recently, an instrument utilizing two and three drift cells in tandem has been described [26]. 

---