MALDI mass spectrometry novel matrix

A recently developed technique that has found extensive use in the characterization of dendrimers, specifically in determining the purity and monodispersity of these novel materials, has been matrix-assisted laser desorption/ionization (MALDI) mass spectrometry [16,17]. For dendrimers grown by the divergent strategy, incomplete functionalization of the periphery can lead to subsequent failure sequences and loss of strict dendritic growth. Observation and quantification of these defects is extremely difficult by other techniques, however MALDI mass spectrometry has been successfully employed by a variety of authors to... 

Kaufmann, R. Matrix-assisted laser desorption ionization (MALDI) mass spectrometry A novel anal3dical tool in molecular biology and biotechnology. J. Biotechnol. 1995, 41, 155-175. 

Use of Matrix Assisted Laser Desorption/ Ionization Imaging Mass Spectrometry (MALDI-IMS) in the Development of Novel Small Molecule Drugs... 

Since then, many novel methods have been developed to analyze gene expression and mutation by using electrochemical techniques [1,2], matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MASS) [3], polymerase chain reaction (PCR) [4,5], bacterial magnetic particles [6], and microbeads [7]. 

The classical area of application of mass spectrometry has been with small volatile compounds, although non-volatile samples could be analysed if they were suitably derivatised. The application of mass spectrometry to large complex molecules like proteins has been made possible by the development of novel ionisation techniques which enable large molecules (> 200 kDa) to be introduced into the mass spectrometer in an intact form suitable for analysis (Siuzdak 1996 Dass 2000). Of the various techniques that have been developed, electrospray ionisation (ESI) and matrix-assisted laser desorption ionisation (MALDI) are the ones best suited for use with macromolecules and macromolecular complexes. 

An impetus in the field of mass spectrometry (MS) analysis occurred in the early 1990s with the invention of two novel and soft ionization methods, electrospray ionization (ESI) by John Fenn and matrix-assisted laser desorption ionization (MALDI) by Koichi Tanaka, who both shared the Nobel Prize in Chemistry in 2002. A second impetus, which is more diffuse, is currently occurring and consists of miniaturization. Whereas the intrinsic sensitivity of mass spectrometers has roughly remained the same for a couple of decades, the amount of material required for recording one spectrum has... 

The hnished product will be subjected to inspection and rigorous testing for identity, uniformity, residual water content, stability, sterility and potency. In addition, all analytical techniques employed in testing these attributes will themselves have been subjected for reliability, reproducibility, experimental uncertainty limits. The biotechnological revolution has resulted in the appearance of ever more rehned and sensitive analytical techniques, mainly novel types of spectroscopy and coupled techniques, based on mass spectrometry, known usually by complex acronyms, e.g. MALDI-TOE-MS (Matrix-Assisted-Laser-Z)esorption-71me-of-Tlight-Mass Spectrometry). Some of the available analytical procedures are treated in more detail in the next chapter. 

The search for novel pharmacological tools in spider venoms requires precise and reproducible methods for the identification of species. Reversed-phase LC and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of crude venoms offers a powerful systematic tool that, in combination with morphological methods, has been used successfully to discriminate among closely related tarantula species. The method allows for the precise, sensitive, and rapid mass fingerprinting of large numbers of... 

Ichiki Y, Ishizawa N, Tamura H, Teramoto K, Sato H, Yoshikawa H. Environmental distribution and novel high-throughput screening of APEO-degrading bacteria using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS). J Pestic Sci. 2008 33(2) 122-7. 

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