Morphologies, methods

In a sense, therefore, there have been two conflicting views with respect to the suitability of formalin as a fixative, in the face of demands that biopsy tissues may be examined not only by traditional morphologic methods, but also by IHC, in situ hybridization (ISFI) and, following extraction procedures, by other molecular methods. Both views recognize that these newer methods do not perform well, or at all, on routinely processed FFPE tissues. One view advocates the development of new fixatives that are molecular friendly, the other view holds that AR-based methods may be employed to achieve accurate valid results of IHC, ISH, and other molecular methods using FFPE tissues. 

It is not intended to describe purely morphological methods of identification in any more detail in this book, for we are concerned with... 

Proceeding from the urban LUC, the calculation of the main aerodynamic characteristics of urban areas such as the roughness length and displacement height, can be performed based on the morphometric or morphologic methods. 

Schwatz J. The diagnosis of deep mycoses by morphological methods. Hum Pathol. 1982 13 519-533. 

The third major class of analytical techniques may be called morphological methods. This identification consists of comparing the form of particles captured with the morphology of particles of known composition. It goes without saying that morphological similarity is a necessary but not always sufficient condition for compositional identity. In spite of this problem this procedure is widely employed mainly in clean atmosphere, since even Aitken size particles can be identified morphologically (A. Meszaros and Vissy, 1974 Butor, 1976). 

The detection of tumor-specific translocations can be used to monitor the response of these tumors to the specific therapy and also as a tool for the detection of minimal residual disease. Different studies demonstrate that the elimination of tumor cells in many hematopoietic malignancies below the level of one neoplastic cell in 10 normal cells predicts a stable remission, whereas a concentration of one neoplastic cell in less than 10 background cells is an indicator for poor prognosis, and considered as a molecular relapse. Note that tumor cell concentration of 1 10 cannot be detected by conventional morphological methods. 

If ITC are found in bone marrow, blood or other organs by non-morphologic methods the symbols i and mol are added to MO in addition to the specification of the anatomical sites e.g. MO (i+, liver) or MO (mol+, blood). 

Analyte Polymer material and morphology Method Response/ Sensitivity Analytical range... 

Nowadays in the forensic science laboratory, large pieces of human (and animal) hairs collected from the crime scene are examined by morphological methods using macroscope, microscope, and electron microscope, and may be compared with sample hairs of humans associated with the crime. Finally, their origins are determined. 

However, it is well known that identification of hair by morphological methods is not always reliable and should be done by a well-trained analyst for maximum reliability. It is difficult to distinguish the suspect s hair from many other hairs left at the crime scene if there is no finding of particular characteristics in the morphology. Therefore, in addition to morphological examination, ABO blood typing from hair can be made, which is helpful in the... 

The search for novel pharmacological tools in spider venoms requires precise and reproducible methods for the identification of species. Reversed-phase LC and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of crude venoms offers a powerful systematic tool that, in combination with morphological methods, has been used successfully to discriminate among closely related tarantula species. The method allows for the precise, sensitive, and rapid mass fingerprinting of large numbers of... 

Physiological, morphological methods Standard methods Miniature commercial systems (e.g., API 20C AUX, rapid IDyeast plus) Chemotaxonomic methods Genus, species Genus, species ... 

The term cytoplasmic DNA applies to DNA species found outside a nucleus either by morphological methods or biochemically, after homogenization and differential centrifugation. In the latter case the cytoplasmic DNA may be contaminated with degraded nuclear DNA. Except for comparatively short polydeoxyribonucleotide components attached to cytochrome b, and the DNA components (I-DNA) described by Bell and co-workers which occur in the cytoplasm of embryonic cells (cf. Section, II,C) extranuclear DNA is mainly localized within the mitochondria and chloroplasts of eukaryotic cells. Since mitochondrial DNA (mt DNA) is the only form of cytoplasmic DNA which is well characterized at the moment we shall concentrate on this. 

Dresel S, Grammerstorff J, Schwenzer K, Brinkbaumer K, Schmid R, Pfluger T, Hahn K (2003) [18F]FDG imaging of head and neck tumours comparison of hybrid PET and morphological methods. Eur J Nucl Med Mol Imaging 30 995-1003... 

The interaction of HDL with the lipid droplets has been further investigated by morphological methods (Fig. 3). Upon lipid accumulation in the macrophage, an intimate contact between the endoplasmic reticulum (ER) and the margin of the lipid droplet can be observed. At the site of contact, due to the induction of phospholipid and apolipoprotein synthesis, lamellar bodies are formed which are surrounded by a newly synthesized membrane. These lamellar bodies consist of free cholesterol, phospholipids, and apolipoproteins including Apo E. From these lamellar bodies, HDL take up cholesterol, Apo E, and phosphoUpids on their route through the cell (Fig. 3b). In the absence of HDL, the lamellar bodies which originate from cytoplasmic lipid droplets, cannot be secreted from the cell. 

Metal oxide Optimal mixing ratio Morphology Method V(°c) Detection range (ppm) Response 5 b,c ppm ... 

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