Liposomes injections

Gregoriadis G, Ryman BB. Fate of protein-containing liposomes injected into rats. An approach to the treatment of storage diseases. Eur J Biochem 1972 24 485. 

I. Yanagihara, K. Inui, G. Dickson, G. Turner, T. Piper, Y. Kaneda, and S. Okada, Expression of full-length human dystrophin cDNA in mdx mouse muscle by HVJ-liposome injection, Gene Ther. 3 549 (1996). 

Doxil (doxorubicin HC1 liposome injection, 2 mg/mL) from Ortho Biotech. 

L.A. Trissel, et al., Compatibility of doxorubicin hydrochloride liposome injection with selected other drugs during simulated Y-site administration. Am. J. Health Sys. Pharmacol. 54 2708-2713, 1997. 

Dezube BJ. Safety assessment Doxil (doxorubicin HCl liposome injection) in refractory AIDS-related Kaposi s sarcoma. Doxil Clinical Series, Vol. 1, No. 2. Menlo Park, California SEQUUS Pharmaceuticals Inc, 1996. 

Bedikian AY et al (2006) Pharmacokinetics and urinary excretion of vincristine sulfate liposomes injection in metastatic melanoma patients. J CKn Pharmacol 46 727-737... 

Ishida T et al (2005) Accelerated blood clearance of PEGylated liposomes following preceding liposome injection Effects of lipid dose and PEG surface-density and chain length of the first-dose liposomes. J Control Release 105 305-317... 

Mice are randomized and liposomes injected IV via tail vein injection. The injection schedule followed is as per a previous study conducted using ceramide liposomes (10), with mice injected at a frequency of one injection per 2 days. 

The encapsulation efficiency of a thalidasine liposome injection was studied using Sephadex G-50 column fluorimetry. The free alkaloid was separated from the liposome particles by the column, with satisfactory recovery of the liposome particles, as well as column reproducibility. The encapsulation efficiency of this particular liposome preparation (139-2) was approximately 93% due to the highly lipophilic property of the alkaloid. The authors concluded that the method was suitable for the quality control of encapsulation efficiency of this polyphase liposome injection [147]. 

In a separate study, the quantity of thalidasine in a polyphase-liposome injection (139-2) was determined by dilution of the injection with EtOH-O.OlN HC1 (4 1), followed by spectrofluorometric determination at 235 nm (for excitation) and at 318 nm (for emission). The recovery was > 96% and the coefficient of variation was < 3%. The polyphase-liposome encapsulation rate was also determined by this method after separation via Sephadex column chromatography [148]. 

Other recent applications Include effects against inflammation with liposomes injected in arthritic joints,H2 anti-tumor effects of encapsulated methotrexate against l5rmphosarcomas resistant to this drug,H3 anti-tumor effects of encapsulated alkylating agents and nucleotide analogs such... 

Five hours after liposome injection, animals are euthanized by an overdose of pentobarbital. 

Intravitreal liposome injection as a means to achieve sustained intraocular levels has been studied extensively. After injection, liposomes appear to be eliminated... 

Fluocinolone acetonide topical 011,0.01% Cytarabine liposome injection... 

Mor[Jiiiie sulfate extended-release liposome injection Cholesterol, dioleoylphosphatidyldioline, dipalmitoyl[ osi atidylglycerol, tric irylin and triolein Control of pain after major surgery [260]... 

Doxorubicin HCl liposome injection (intravenous infusion) MPEG-DSPE, fully hydrogenated soy [ os[ atidylchohne, cholesterol Treatment of AIDS-related Kaposi s sarcoma [260,261]... 

Bupivacaine liposome injectable suspension Cholesterol, dipalmitoyl[ os[ atidylglycerol, tric irylin, diemcoylphosphatidylcholine Management of postsurgical pain [262]... 

Vincristine sulfate liposome Injection Cholesterol Treating Philadelphia chromosome negative acute lymphoblastic leukemia [263]... 

Dasta J et al. Bupivacaine liposome injectable suspension compared with bupivacaine HCl for the reduction of opioid burden in the postsurgical setting. Current Medical Research and Opinion. 2012 28(10) 1609-1615. 

Gregoriadis, G., and Ryman, B. E. (1972) Lysosomal localization of enzyme-containing liposomes injected into rats. Biochem. J. 129, 123-133. 

A liposomal injection solution of verteporin (Visudyne ) is formulated with egg phosphatidyl-glycerol and dimyristoylphosphatidyl choline. The freeze-dried powder, contains apart from the liposomes, verteporin, lactose as lyoprotector, and osmolality adjusting excipients. By reconstitution with water an opaque dark green injectable solution is generated, which is injected intravenously within the scope of photodynamic therapy. 

Enzyme replacement is the logical approach for the treatment of lysosomal storage diseases (7) but use of an "address label" for the injected molecule is required (8). Antibodies (5,6), hormones (9), lectins (10), glycoconjugates (11), virus coats (12), bacterial toxins (13) might be used as vectors. Liposomes injected in the blood stream are taken up rapidly by cells in the reticuloendothelial system (3). 

Hydrogenated soy phosphatidylcholine-cholesterol-BST liposomes injected into dairy cattle increased weekly milk production (Weiner et al, 1989). 

More than thirty years have passed since Bangham described a liposome for the first time (14). Liposomes had been considered to be a carrier for the delivery of dmg because of non-toxic ceU-Uke stmcture. However the original liposomes possessed a drawback as the liposomes injected intravenously into animals were rapidly cleared primarily by the RES. Consequently they disappear before reaching the tumor tissues and exerting their ceU-kiUing effect. Thus requirements for liposomes with longer circulation times and improved avoidance of RES capture, became prerequisite, which was later accomplished by the development of stealth Uposome for cytotoxic dmg delivery (6-10). In the cases of stealth... 

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