Kaposi’s sarcoma associated herpesvirus KSHV

Same laser for Raman and one optical tweezers 780 nm Healthy and tumor infected cells causing Kaposi s sarcoma-associated herpesvirus (KSHV) Intensities of the bands at 1,004, 1,093, and 1,663 cm were altered Detection of cancer infected cells. Large study with 85% sensitivity and 92.5% specificity in blind tests to diagnose cancer. Biological relevance of KSHV infection upon cell signaling was investigated... 

Various viruses encode proteins with sequenee homology to host proteins which are known to be involved in host defense functions. Viruses pirate and modify key immunoregulatory molecules, by use of molecular mimicry, to elude the Immune system (Murphy, 1997). Viruses also encode proteins that exploit or alter their host cells, replicate or induce migration for virus dissemination. Interestingly, DNA viruses such as the Herpesviruses (Cytomegalovirus (CMV), human herpesvirus (HHV-6 and 7), herpesvirus Saimiri (HVS) and Kaposi s sarcoma-associated Herpesvirus (KSHV)) all express GPCRs (Table 2). 

Geras-Raaka E. Kaposi s sarcoma-associated herpesvirus (KSHV) chemokine vMIP-II and human SDF-lalpha inhibit signaling by KSHV G protein-coupled receptor. Biochem Biophys Res Commun 1998 253(3) 725-7. 

ORF, open-reading frame NA, not available KS, Kaposi s sarcoma KSHV, Kaposi s sarcoma-associated herpesvirus GPCR, G protein-coupled receptor CMV, cytomegalovirus MCMV, mouse cytomegalovirus MCK, murine cytomegalovirus chemokine CCL5, CC chemokine ligand 5 IFN, interferon IL, interleukin NF-kB, nuclear factor kB Tp, T helper NK, natural killer. 

Viral particles of HCMV and Kaposi s sarcoma-associated herpesvirus/human herpesvirus 8 (KSHV/HHV-8) have recently been examined. During the her-pesviral replicative cycle, different viral particles are formed. For HCMV, this includes mature, infectious virions, noninfectious enveloped particles, and dense bodies [6]. Similarly for KSHV, only a portion of the produced virus particles is infectious [40]. Therefore, analysis of infectious virions requires their separation from the noninfectious and immature forms. Density ultacentrifugation gradients are typically used to separate the various forms. Each fraction can be analyzed by electron microscopy to determine the level of purity [6,41] or by assaying for viral DNA and an envelope glycoprotein [40]. 

KSHV, also termed human herpesvirus 8 (HHV-8), is a 7 herpesvirus that is implicated as a transforming agent in the pathogenesis of Kaposi s sarcoma (KS) and other proliferative disorders such as primary effusion B-cell lymphomas (Ganem, 1997). KS is the most common malignancy in HIV-infected individuals, but has also been detected in non HIV-infected individuals. KS is a highly angiogenic multicentric tumor associated with abundant... 

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