Open-reading frames

As the complete genomes of more and more species are sequenced, the determination of the function of previously unidentified open reading frames is becoming an increasing and challenging problem. The possibility of... 

The cDNA encoding the luciferase of Renilla reniformis has been obtained and expressed in Escherichia coli (Lorenz et al., 1991). The cDNA contained an open reading frame encoding a 314-amino acid sequence. The recombinant Renilla luciferase obtained had a molecular weight of 34,000, and showed an emission maximum at 480 nm in the luminescence reaction of coelenterazine, in good agreement with the data of natural Renilla luciferase. 

Neuronal NO synthase (nNOS) is constitutively expressed in neurons of the brain. Its activity is regulated by Ca2+ and calmodulin. Half-saturating L-arginine concentrations are around 2 pM. cDNAs encoding nNOS have been cloned from rat and human brain. The open reading frame of human nNOS consists of 4299 bp, corresponding to 1433 aa. This predicts a protein of 160 kDa, which is in accordance with the molecular mass of the purified protein. 

Computer algorithms facilitate identification of the open reading frames that encode a given protein by using partial sequences and peptide mass profiling to search sequence databases. 

The open reading frame of the mRNA is the series of codons, each specifying a certain amino acid, that determines the precise amino acid sequence of the protein. 

DNA sequencing Establishes the highest resolution physical map. Identifies open reading frame. Facilities with many high throughput instruments could sequence millions of base pairs per day. 

Figure 2. Restriction map of the A. aculeatus rhgA genomic clone plM803. pIM803 contains a 3.9-kb chromosomal Ba/nHI-Sa/1 fragment in pBluescriptSK(-l-). The open reading frame is indicated as closed bars. 5 - and 3 - untranslated sequences and introns are given as open boxes.

Uptake was inducible by the substrate, did not occur with fructose-grown cells, and was inhibited by cyanide that prevents development of a protomotive force, and by the protophore carbonylcyanide-3-chlorophenylhydrazone (Leveau et al. 1998). The protein involved was encoded by an open reading frame on the plasmid designated tfdK. 

B - An interrupted open reading frame - An uninterrupted open reading frame... 

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