Isolation of novel

Simons, J. N et al. (1996). Isolation of novel virus-like sequences associated with human hepatitis. Nat. Med. 1, 564-569. 

Yoshikawa T et al. Isolation of novel mouse genes differentially expressed in brain using cDNA microarray. Biochem Biophys Res Commun 2000 275 532-537. 

The development of new chemotherapeutic agents for a particular disease state continues to depend to a significant degree on the discovery of a lead compound , which can be further developed into a viable drug candidate. A very important aspect of this process is to find novel substances that will provide a new mode of action for the treatment of a specific disease. From a historical standpoint, the isolation of novel substances from natural sources has often provided this lead compound. The number of new drugs and their origin for the period 1981-2002 has recently been summarized [1], and, of 79... 

Straub, K. L., Hanzlik, M. and Buchholz-Cleven, B. E. E. (1998). The use of biologically produced ferrihydrite for the isolation of novel iron-reducing bacteria, Syst. Appl. Microbiol., 21, 442-449. 

Inflammation is associated with various diseases such as rheumatoid arthritis, cancer, myocarditis, arteriosclerosis, bowel diseases, multiple sclerosis, asthma, and many others. While several inflammatory markers are commonly expressed during any inflammatory disorder, some are symptom specific. Therefore, the gene array data will be particularly helpful in indicating the appropriate disease model for subsequent preclinical and clinical tests. Only functional, active extracts with potentially safe and novel modes of actions may then be subjected to labor-intensive large-scale extraction, fractionation, characterization, and isolation of novel bioactive components. We believe that the strategy as described schematically in Figure 4.1 will allow efficient use of plant extracts and other natural resources toward identification of novel drug leads for human health care. 

S Gupta, C Montilor, Y Hwang. Isolation of novel beauvericin analogues from the fungus Beauveria bassiana. J Natural Prod 58 733-738, 1995. 

Degradation of the icosahedral 1,2- and 1,7-C2Bi0Hi2 isomers with strong base to produce the nido eleven-vertex anions 7,8- and 7,9-CoB9Hi2-has been previously discussed and is an important route to the preparation of twelve-vertex monometallocarboranes. The discovery that similar reactions could be performed on metallocarboranes led to the isolation of novel chains of metal atoms bridged by carborane groups and to the development of the polyhedral contraction and polyhedral subrogation reactions. 

The first chapter of this volume by Balch discussed the current progress in two-coordinate luminescent gold(I) complexes. This class of complexes is well-known to show weak metal- metal interactions that lead to the isolation of novel architectures and polymorphism from relatively simple building blocks and the appearance of unique electronic absorption and emission spectroscopic features. The effect of the environment, such as solvents and counter ions, on the luminescence behavior of a number of two-coordinate gold(I) complexes was discussed. 

Olsen, M. J., Stephens, D., Griffiths, D., Daugherty, P. S., Georgiou, G., and Iverson, B. L. (2000). Isolation of novel enzymes from large libraries. Submitted. 

Osbourn JK, Derbyshire EJ, Vaughan TJ, Field AW, Johnson KS, Pathfinder selection in situ isolation of novel antibodies, Immunotech., 3 293-302, 1998. 

Isolation of novel free carbenes is not always trivial, mainly due to difficulties with decomposition and the need to handle free NHCs under inert atmosphere conditions. In this context, the use of protected forms of free NHC carbenes has appeared as a useful alternative to the preparation of NHC complexes. N-Heterocyclic rings containing alkoxide or trichloromethyl groups, such as shown in Scheme 7, can be considered as NHC-adducts in the sense that they can readily eliminate alcohol or chloroform to unmask the carbene, which would then coordinate to the metal. 

A. M. Maszenan, R. J. Seviour, B. K. Patel, P. Schumann, J. Burghardt, Y. Tokiwa and H. M. Stratton (2000). Three isolates of novel polyphosphate-accumulating gram-positive cocci, obtained from activated sludge, belong to a new genus, Tetrasphaera gen. nov., and description of two new species, Tetrasphaera japonica sp. nov. and Tetrasphaera australiensis sp. nov. Int. J. Syst. Evol. Microbiol., 50, 593-603. 

Stable as well as transitory perchlorate-coordinated species are convenient intermediates for the isolation of novel complexes. With four potential donor sites, the role of C104 acting as a bridging ligand perhaps has interesting possibilities yet to be revealed. 

B. L. Iverson. Function-based isolation of novel enzymes from a large library. Nat Biotechnol (2000) 18(10) 1071-4. 

In the case of AGT, we have demonstrated the isolation of novel mutants that show enhanced function, variants that are more resistant to inhibitors, and mutants that have altered substrate specificity. Mutants in each category have important applications in structure/function studies, gene therapy, and mutation analysis. 

Olsen Ml, Stephens D, Griffiths D, Daugherty P, Georgiou G, Iverson BL. Eunction-based isolation of novel enzymes from a large Ubrary. Nat. Biotechnol. 2000 18 1071-1074. 

Suzuki, S., Isolation of novel disaccharides from chondroitin sulfates. /. Biol. Chem. 235, 3580-3588 (1960). 

Haider T, Pawelec G, Kirkin AF et al. Isolation of novel HLA-DR restricted potential tumor-associated antigens from the melanoma cell line FM3. Cancer Res 1997 57(15) 3238-44. 

Baer, K.N. and P. Thomas. Isolation of novel metal-binding proteins distinct from metallothionein from spotted seatrout (Cynoscion nebulosus) and Atlantic croaker (Micropogonias undulatus) ovaries. Mar. Biol. 108 31-37, 1991. 

Beullens M, Van Eynde A, Stalmans W et al (1992) The isolation of novel inhibitory polypeptides of protein phosphatase 1 from bovine thymus nuclei. J Biol Chem 267 16538-16544... 

Since DFRC does not scramble the (5-carbon stereochemistry, the recovery of optically inactive (5-5 (Figure 2.10d) and (5-(5 DFRC dimers very elegantly confirmed the racemic nature of lignins [99]. The isolation of novel arylisochroman trimers from DFRC-degraded loblolly pine wood sample supports the occurrence of... 

Isolation of Novel Pyranoanthocyanins from Pinotage Wines... 

CHROMONE ALKALOIDS Isolation of Novel Compounds Cassiadinine... 

Isolation of Novel Sesquiterpenoid MMP-3 Inhibitors from an Acid Mine Waste Extremophile... 

Kraus, M.H. Aaronson, S.A. Detection and isolation of novel protein-tyrosine kinase genes employing reduced stringency hybridization. Methods EnzymoL, 200, 546-557 (1991)... 

Katsanis N., Fitzgibbon J., Fisher E.M. (1996). Paralogy mapping identification of a region in the human MHC triplicated onto human chromosomes 1 and 9 allows the prediction and isolation of novel PBX and NOTCH loci. Genomics 35 101-108. 

In this paper, we describe the production, detailed structure, enzymatic degradation and isolation of novel oligosaccharides, and also derivatization, such as 3,6-anhydro-elsinan. Some of rheological properties and potential utilization of this glucan will also be discussed. 

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