Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Interfacial protein adsorption

Sadana A. Interfacial protein adsorption and inactivation. Bioseparation 1993 3(5) 297-320. [Pg.286]

Different models have been used to fit the interfacial protein adsorption data [76] and include, but are not hm-ited to, Langmuir [56, 58, 71, 73, 77], Freundlich [78, 79], and random sequential adsorption (RSA) [80, 81] models. Equilibrium adsorption of different proteins at the silicone oil/water interface, under different solution conditions, has been seen to occur in a monolayer as indicated by the Langmuir [51,55] (Figure 25.6) or RSA model [30,54]. [Pg.387]

J. D. Andrade, Surface and Interfacial Aspects of Biomedical Polymers, Vol. 2, Protein Adsorption, Plenum, 1985. [Pg.425]

In line with the Gibbs adsorption equation (equation 3.33 in chapter 3), the presence of thermodynamically unfavourable interactions causes an increase in protein surface activity at the planar oil-water interface (or air-water interface). As illustrated in Figure 7.5 for the case of legumin adsorption at the n-decane-water interface (Antipova et al., 1997), there is observed to be an increase in the rate of protein adsorption, and also in the value of the steady-state interfacial pressure n. (For the definition of this latter quantity, the reader is referred to the footnote on p. 96.)... [Pg.241]

A situation that commonly occurs with food foams and emulsions is that there is a mixture of protein and low-molecular-weight surfactant available for adsorption at the interface. The composition and structure of the developing adsorbed layer are therefore strongly influenced by dynamic aspects of the competitive adsorption between protein and surfactant. This competitive adsorption in turn is influenced by the nature of the interfacial protein-protein and protein-surfactant interactions. At the most basic level, what drives this competition is that the surfactant-surface interaction is stronger than the interaction of the surface with the protein (or protein-surfactant complex) (Dickinson, 1998 Goff, 1997 Rodriguez Patino et al., 2007 Miller et al., 2008 Kotsmar et al., 2009). [Pg.324]

Specific formulation strategies need to be employed for macromolecule compounds. An excellent review of protein stability in aqueous solutions has been published by Chi et al. (92). In addition to solution stability of proteins and peptides, aerosolization may result in significant surface interfacial destabilization of these compounds if no additional stabilization excipients are added. This is due to the fact that protein molecules are also surface active and adsorb at interfaces. The surface tension forces at interfaces perturb protein structure and often result in aggregation (92). Surfactants inhibit interface-induced aggregation by limiting the extent of protein adsorption (92). [Pg.243]

It is commonly stated that the first readily observable event at the interface between a material and a biological Quid is protein or macromolecule adsorption. Clearly other interactions precede protein adsorption water adsorption and possibly absorption (hydration effects), ion bonding and electrical double layer formation, and the adsorption and absorption of low molecular weight solutes — such as amino acids. The protein adsorption event must result in major perturbation of the interfacial boundary layer which initially consists of water, ions, and other solutes. [Pg.3]

An excellent comprehensive review of all theoretical and practical aspects of dynamic and static interfacial tension measurements written by the most prolific authors in the field of protein adsorption. Contains a wealth of additional references that the interested reader may consult to gain additional understanding of the field of research. [Pg.646]

Interestingly, protein adsorption is also a field of biological interfacial chemistry which parallels that of synthetic materials at the solid - liquid interface. A number of spectroscopic advances have been made which allow FT-IR to be used in kinetic monitoring of protein adsorption on metals and "biocompatible" polymers. In addition to providing in - situ measurements of total adsorbed protein, FT-IR can also yield information about perturbation of protein secondary structure in adsorbed layers. [Pg.4]

Thus, protein adsorption and cell adhesion occur for various reasons and in different appearances. When surfaces of living systems are involved, specific recognition mechanisms undoubtedly play crucial roles. Nevertheless, since we are dealing with a rather general phenomenon, it is likely that these specific interactions are superimposed on a generic interaction mechanism. Bioadhesion and adsorption is very complicated from a physical chemical point of view. Interfacial tensions, wetting and electrical properties of the surfaces are prominently involved. [Pg.160]

Protein function at solid-liquid interfaces holds a structural and a dynamic perspective [31]. The structural perspective addresses macroscopic adsorption, molecular interactions between the protein and the surface, collective interactions between the individual adsorbed protein molecules, and changes in the conformational and hydration states of the protein molecules induced by these physical interactions. Interactions caused by protein adsorption are mostly non-covalent but strong enough to cause drastic functional transformations. All these features are, moreover, affected by the double layer and the electrode potential at electrochemical interfaces. Factors that determine protein adsorption patterns have been discussed in detail recently, both in the broad context of solute proteins at solid surfaces [31], and in specific contexts of interfacial metalloprotein electrochemistry [34]. Some important elements that can also be modelled in suitable detail would be ... [Pg.135]

LTI pyrolytic carbon is one of the very few synthetic materials generally accepted as suitable for long-term blood contact applications (1 ). Although a number of hypotheses have been formulated with respect to the blood tolerability of materials, a general theory or mechanism is not yet available. Nyilas, et al., ( ) have shown that in certain situations the local hemodynamics can play a predominant role, while in most cases the solid-blood interfacial properties have been shown to be equally important (2, 3). It is assumed that understanding the plasma protein adsorption processes on solids used for blood-contact applications will lead to a better understanding of solid-blood interactions (, 2, ... [Pg.383]

Some of the areas where interfacial protein layers dominate the boundary chemistry are reviewed, and we introduce some nondestructive armlytical methods which can be used simultaneously and/or sequentially to detect and characterize the microscopic amounts of matter at protein or other substrates which spontaneously acquire protein conditioning films. Examples include collagen and gelatin, synthetic polypeptides, nylons, and the biomedically important surfaces of vessel grafts, skin, tissue, and blood. The importance of prerequisite adsorbed films of proteins during thrombus formation, cell adhesion, use of intrauterine contraceptives, development of dental adhesives, and prevention of maritime fouling is discussed. Specifics of protein adsorption at solid/liquid and gas/liquid interfaces are compared. [Pg.1]

Because direct electrochemistry is observed only after the problems of interfacial specificity, compatibility, and denaturation have been overcome, it should provide us with a most powerful tool for investigating protein adsorption at surfaces. The studies of genetically engineered cytochrome c variants described in Section II serve as an illustration of this application. [Pg.371]


See other pages where Interfacial protein adsorption is mentioned: [Pg.244]    [Pg.397]    [Pg.89]    [Pg.129]    [Pg.129]    [Pg.138]    [Pg.387]    [Pg.388]    [Pg.391]    [Pg.244]    [Pg.397]    [Pg.89]    [Pg.129]    [Pg.129]    [Pg.138]    [Pg.387]    [Pg.388]    [Pg.391]    [Pg.542]    [Pg.137]    [Pg.68]    [Pg.338]    [Pg.116]    [Pg.79]    [Pg.68]    [Pg.96]    [Pg.308]    [Pg.312]    [Pg.44]    [Pg.44]    [Pg.48]    [Pg.622]    [Pg.68]    [Pg.19]    [Pg.156]    [Pg.384]    [Pg.330]    [Pg.1334]    [Pg.124]    [Pg.429]    [Pg.20]    [Pg.20]    [Pg.231]    [Pg.251]    [Pg.167]   
See also in sourсe #XX -- [ Pg.352 ]




SEARCH



Interfacial adsorption

Protein adsorption

© 2024 chempedia.info