Injection manual system

Figure 11(A) shows a principle sketch of a SEC set-up. The eluent (solvent) is pumped at a constant flow rate through the system. A small amount of polymer solution (typically 200 pL) is injected manually or with an autosampler. The main part comprises a set of columns (typically 3-4 columns+pre-column) typically packed with microporous styrene-divinylbenzene, porous glass, or silica. In the routine analytical laboratory it is especially useful to have a pre-column in order to collect impurities that might be present in the samples. If many different samples are to be analyzed, it is necessary to check the reliability of the columns frequently to avoid artefacts due to residues from previous samples still held on the column. In order to avoid problems, samples should be purified before they...

Typical protein precipitation procedures use one volume of plasma plus three to six volumes of acetonitrile or methanol (or a mixture) with the internal standard at an appropriate concentration for the assay. Poison et al.102 reported that protein precipitation using acetonitrile eliminates at least 95% of the proteins after filtration or centrifugation, the supernatant can often be directly injected into the HPLC/MS/MS system. Usually this step is performed using 96-well plates that are ideal for semi-automation of sample preparation. Briem et al.103 reported on a robotic sample preparation system for plasma based on a protein precipitation step and a robotic liquid handling system that increased throughput by a factor of four compared to a manual system. 

M 20] [P 19] The pTAS module is made for performing colorimetric analyses as typically applied in cuvette tests, e.g. for on-site water analysis [30], A continuous test replaces copious manual pipetting of the sampling volumes. The module consists of a micro flow restrictor, a micro mixer and an optical microcuvette for colorimetric analysis. The sample is injected by a conventional FIA (flow injection analysis) system. By close connection, a dead volume of only 2.2 pi is given. 

Apart from continuous sample aspiration also flow injection and discrete sampling can be applied (see Section 3.1), both of which deliver transient signals. In the latter case 10-50 pi aliquots can be injected manually or with a sample dispenser into the nebulization system, as was first proposed by Ohls et al. [125] and described by Bemdt et al. (see Ref. [126]). The approach is especially useful for preventing clogging in the case of sample solutions with high salt contents, for the analysis of microsamples as required in semm analysis or when aiming at the... 

Craig, I.P., Matthews, G.A. and Thornhill, E.W. (1993) Fluid injection metering system for closed pesticide delivery in manually operated sprayers. Crop Protection 12, 549-553. 

With a manual system, the sample is injected via a syringe through the needle port into a fixed volume loop. The handle is then turned to the inject position and the sample is swept out of the loop, with the aid of the mobile phase, and onto the column. The syringe is then removed and the handle is returned to the load position. Loop sizes can vary from 2 pL to 5 mL. Some manual systems allow operation in both complete- and partial-fill modes while maintaining accurate delivery of sample volume. There is the risk of error in relation to the volume delivered when using a partial-fill method and only those systems designed for that purpose should be used. 

The major disadvantage of a manual system is that the operator must be present to make the injection. This can be time consuming and also means that the system cannot be used when there are no operators present, such as overnight and during lunch and dinner breaks. In the forensic environment, it is often necessary to run many samples at a time therefore, the manual systems have been replaced by automated systems capable of handling up to 100 samples at any one time. 

Sample injection manual injection and autoinjection are available however, more errors might be expected with a manual injection system. 

One potentially effective combination can be that of SI A and MSFIA (Figure 1.28). In fact, the latter allows all preliminary operations on the sample (e. g. preconcentration on a solid support, photooxidation) to be conducted by mimicking the work of manual systems while the latter can contribute with increased precision in injecting the reagents in parallel via a multisyringe and hence more efficient mixing with the sample. 

An automated system called the short-path thermal desorber (SPTD) (Fig. 2) was developed jointly by researchers at Rutgers University and Scientific Instrument Services of Ringoes, New Jersey (an independent company, separate from the previously mentioned SIS) (14). This instrument is placed on top of the GC injection port and can be adjusted to allow conventional injections without disconnecting the unit. The sample is placed in a glass-lined stainless steel tube and held in place with glass wool. An injection needle is affixed to one end and the other end is screwed into the SPTD. The injection is made pneumatically, and the sample is not heated prior to injection. Control of the temperature, desorption time, and equilibration are provided by an electronic controlling unit. An automated system has an inherent advantage over manual systems for improved reproducibility from one run to the next. Quantitative data with a precision of less than 5% STD has been reported (15). 

SPME has been utilized for deterrnination of pollutants in aqueous solution by the adsorption of analyte onto stationary-phase coated fused-siUca fibers, followed by thermal desorption in the injection system of a capillary gas chromatograph (34). EuU automation can be achieved using an autosampler. Eiber coated with 7- and 100-p.m film thickness and a nitrogen—phosphoms flame thermionic detector were used to evaluate the adsorption and desorption of four j -triazines. The gc peaks resulting from desorption of fibers were shown to be comparable to those obtained using manual injection. 

In some BWR transient scenarios, the high pressure injection systems are postulated to fail. To make use of the low pressure injection system, it is necessary to depressurize the reactor coolant system, a function performed by the automatic depressurization system (ADS). In the scenario considered, ADS actuation is manual because the signals for automatic initiation of the system are not present. 

Figure 2. Schematic representation of the reactor system computer-controlled pumps (PI, P2) pump controllers (fc) reactor (CSTR) reception vessel valves (S1-S4) monomer and initiator storage vessels (Tl, T2). (a) Digital input from GPC injection valve (b) analogue output from GPC (c, d) digital outputs to recorder chart drive and event marker (e, f) analogue outputs for pump set-point adjustment (g,h) reactor feeds (i) reactor output (j-m) digital outputs to reception system valves (n) manual sampling of products by GPC,...

The oranges were washed, chopped in a meat mincer and homogenised by a Fryma mill. Water (0.6 volumes) were added before the slurry was heat treated by steam injection at 100°C for 2 minutes. The enzyme treatment was carried out for 1 hour at 40°C with 10 lU/g slurry of PME and 25 pg enzyme protein/g slurry of the other enzymes for each of the enzymes. The gelated orange slurry were treated at 85°C for 3 minutes to inactivate the enzymes before the strength of the gel was measured by a SMS TeJrture Analyser TA-XT2 (Stable Micro Systems, XT. RA Dimensions, Operations Manual versions) by compression analysis using a flat cylinder (20 mm dia.) with a speed of 2 mm/s. The force to provide a 20% compression was recorded. 

If simple sample pretreatment procedures are insufficient to simplify the complex matrix often observed in process mixtures, multidimensional chromatography may be required. Manual fraction collection from one separation mode and re-injection into a second mode are impractical, so automatic collection and reinjection techniques are preferred. For example, a programmed temperature vaporizer has been used to transfer fractions of sterols such as cholesterol and stigmasterol from a reversed phase HPLC system to a gas chromatographic system.11 Interfacing gel permeation HPLC and supercritical fluid chromatography is useful for nonvolatile or thermally unstable analytes and was demonstrated to be extremely useful for separation of compounds such as pentaerythritol tetrastearate and a C36 hydrocarbon standard.12... 

Hardware requirements — The system controller responsible for synchronizing the events is defined as LC System 1. It requires at least two time event outputs to trigger the injection of LC System 2 and start MS data collection. If MS fails, the injection of LC System 1 should be inhibited. Autosampler with ready-in, alarm-in, and stop inputs indicate capability to be stopped remotely. The autosampler of LC System 2 must be able to prepare a sample before the run from LC System 1 is finished and hold the sample in the injector loop until an injection signal is received. A manual injection input devices indicates that the autosampler can perform the required function. 

A patented water injection system has been devised for extinguishing oil and gas well fires in case of a blowout. The "Blowout Suppression System" (BOSS) consist of finely atomized water injected to the fluid stream of a gas and oil mixture before it exits a release point. The added water lowers the flame temperature and flame velocities thereby reducing the flame stability. In the case where the flame cannot be completely dissipated, the fire intensity is noticeably deceased, preserving structural integrity and allowing manual intervention activities. A precaution in the use of such a device is that, if a gas release fire is suppressed but the flow is not immediately isolated, a gas cloud may develop and exploded that would be more destructive that the pre-existing fire condition. 

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