Infrared -MALDI

Dondi, F., Bassi, A., Cavazzini, A., Pietrogrande, M.C. (1998). A quantitative theory of the statistical degree of peak overlapping in chromatography. Anal. Chem. 70, 766. Eckerskom, C., Strupat, K., Schleuder, D., Hochstrasser, D.F., Sanchez, J.-C., Lottspeich, F., Hillenkamp, F. (1997). Analysis of proteins by direct-scanning infrared-MALDI mass spectrometry after 2D-PAGE separation and electroblotting. Anal. Chem. 69, 2888. Expasy,http //www.expasy.ch. [Pg.89]

Berkenkamp, S. Menzel, C. Hillenkamp, F. Dreisewerd, K. Measurements of Mean Initial Velocities of Analyte and Matrix Ions in Infrared MALDI-MS. J. Am. Soc. Mass Spectrom. 2002, 75, 209-220. [Pg.435]

Hillenkamp, F. Mechanisms of Energy Deposition in Infrared MALDI-MS. Int. J. Mass Spectrom. 2001,207,73-96. [Pg.435]

Atmospheric pressure chemical ionization Atmospheric pressure infrared MALDI mass spectrometry... [Pg.400]

The ionization methods reported for IMS included MALDI [41,76-80], Secondary Ion Mass Spectrometry (SIMS) [19, 81-86], Matrix-enhanced (ME)-SIMS [87, 88], Desorption Electrospray Ionization (DESI) [89-99], Nanostructure Initiator Mass Spectrometry (NIMS) [100-102], Atmospheric Pressure Infrared MALDI Mass Spectrometry (AP-IR-MALDI-MS) [103], Laser Ablation-inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) [104-106], Laser Desorption Postionization (LDPI) [107], Laser Ablation Electrospray Ionization Mass Spectrometry (LAESI) [108, 109], and Surface-assisted Laser Desorption/ioniza-tion Mass Spectrometry (SALDI) [110-112], Another method was called probe electrospray ionization (PESI) that was used for both liquid solution and the direct sampling on wet samples. [Pg.405]

Li Y, Shrestha B, Vertes A (2007) Atmospheric pressure molecular imaging by infrared MALDI mass spectrometry. Anal Chem 79 523-532. doi 10.1021/ac061577n... [Pg.419]

S. Berkenkamp, F. Kirpekar, and F. Hillenkamp, Infrared MALDI mass spectrometry of large nucleic acids, Science 281, 260-262 (1998). [Pg.479]

Berkenkamp, S. and Hillenkamp, F. (2005) Infrared MALDI with an orthogonal ion extraction TOP for high performance analysis of large proteins. Proceedings, 53rd American Society for Mass Spectrometry and Allied Topics, San Antonio, Texas, June 5-9, 2005. [Pg.99]

This means that the lateral resolution can be better than the diffraction limit of the chosen laser wavelength. It has been shown that a lateral resolution a factor of 3 below the diffraction limit is possible in infrared MALDI microscope mode imaging [16]. [Pg.139]

Several other ionization methods and MS technologies have been investigated for possible use for imaging. Atmospheric pressure infrared MALDI (AP IR-MALDI) and mid-infrared laser ablation with electrospray ionization (LAESI) have been reported and use focused laser radiation for ambient sampling with no sample preparation or chemical modification required. In these... [Pg.12]

Li, Y., Shrestha, B., Vertes, A. (2008) Atmospheric pressure infrared MALDI imaging mass spectrometry for plant metabolomics. Anal Chem, 80, 407—420. [Pg.171]

DP Degree of polymerisation FT-IR Fourier transform- infrared MALDI-TOF Matrix assisted laser desorption/ionisation-time of flight GPC Gel permeation chromatography SEC Size exclusion chromatography SEM Scanning electron microscopy SEM Scanning Electron Microscopy ... [Pg.439]

Leisner, a. Rohlfing, A. Rohung, U. Dreisewerd, K. Hillenkamp, F. Time-resolved imaging if the plume dynamics in infrared MALDI with a glycerol matrix. J. Phys. Chem. B. 2005, 109, 11661—11666. [Pg.176]

Feldhaus, D. Menzel, C. Berkenkamp, S. Hillenkamp, F. Influence of the laser fluence in infrared MALDI with a 2.94 mm Er YAG laser and a flat-top beam profile. J. Mass Spectrom. 2000,35,1320-1328. [Pg.181]

Figure 6.5. Infrared MALDI of double-stranded DNA. (Reproduced with permission from reference 76).

Liquid chromatography (LC) encompasses several techniques, which includes HPLC and SEC (sometimes referred to as GPC). Many variants of and developments from these techniques exist, and are used in the study and analysis of polymer degradation/oxidation. As discussed earlier, SEC is often coupled with MALDI-ToF-MS to facilitate the identification of the products of polymer degradation. SEC has also been coupled with mid-infrared detection and similarly used for studies of polymer degradation. SEC/GPC is discussed further below. [Pg.443]

DGE a AC AMS APCI API AP-MALDI APPI ASAP BIRD c CAD CE CF CF-FAB Cl CID cw CZE Da DAPCI DART DC DE DESI DIOS DTIMS EC ECD El ELDI EM ESI ETD eV f FAB FAIMS FD FI FT FTICR two-dimensional gel electrophoresis atto, 10 18 alternating current accelerator mass spectrometry atmospheric pressure chemical ionization atmospheric pressure ionization atmospheric pressure matrix-assisted laser desorption/ionization atmospheric pressure photoionization atmospheric-pressure solids analysis probe blackbody infrared radiative dissociation centi, 10-2 collision-activated dissociation capillary electrophoresis continuous flow continuous flow fast atom bombardment chemical ionization collision-induced dissociation continuous wave capillary zone electrophoresis dalton desorption atmospheric pressure chemical ionization direct analysis in real time direct current delayed extraction desorption electrospray ionization desorption/ionization on silicon drift tube ion mobility spectrometry electrochromatography electron capture dissociation electron ionization electrospray-assisted laser desorption/ionization electron multiplier electrospray ionization electron transfer dissociation electron volt femto, 1CT15 fast atom bombardment field asymmetric waveform ion mobility spectrometry field desorption field ionization Fourier transform Fourier transform ion cyclotron resonance... [Pg.11]

From the analytical point of view, reactions were efficiendy monitored using a combination of FTIR (Fourier transform infrared), TLC (thin-layer chromatography), and MALDI-TOF mass spectroscopy analysis of crudes resulting from the cleavage of small resin samples. [Pg.79]

Both LDI and MALDI make use of the absorption of laser light by a solid sample layer. The energy uptake upon laser irradiation then causes evaporation and ionization of the sample. Wavelengths ranging from ultraviolet (UV) to infrared (IR) have been employed, e.g., nitrogen lasers (337 nm), excimer lasers (193, 248, 308 and 351 nm), Q-switched, frequency-tripled and quadrupled Nd Yag lasers (355 and 266 nm, respectively), [24] Er Yag lasers (2.94 pm) [24,25] and TEA-CO2 lasers (10.6 pm). [16,26]... [Pg.411]

Nordhoff, E. Ingendoh, A. Cramer, R. Overberg, A. Stahl, B. Karas, M. Hillenkamp, F. Crain, P.F. MALDI-MS of Nucleic Acids With Wavelengths in the Ultraviolet and Infrared. Rapid Commun. Mass Spectrom. 1992, 6, 771-776. [Pg.434]

In the MALDI technique a pulsed laser beam strikes a solid sample and heats, vaporizes, and ionizes compounds with little decomposition.201-209 Proteins or other biopolymers are mixed with a "matrix" Fiat absorbs the heat of Fie laser beam. The protein sample together with Fie matrix is dried. Most proteins form crystals and Fie laser beam is directed toward individual protein crystals or aggregates. Various materials are used for the matrix. Compounds as simple as glycerol, succinic acid, or urea can be used with an infrared laser. For proteins an ultraviolet nitrogen laser tuned to 337 nm is usually employed with an ultraviolet light-absorbing matrix such as hydroxy-benzoic acid, 2,5-dihydroxybenzoic acid, a-hydroxy-... [Pg.112]

The two additives have heen identified hy matrix assisted laser desorption ionisation mass spectrometry (MALDI-MS) and attenuated total reflection infrared spectroscopy (ATR). [Pg.186]