Immunocytochemical studies

It is the a2A/D-adrenoceptor that predominates in the locus coeruleus and this subtype seems to be responsible for reducing neuronal excitability and transmitter release. Strangely, immunocytochemical studies suggest that most a2c-receptors are intracellular. The explanation for this finding and its functional implications are as yet unknown but it could reflect differences in intracellular trafficking of different receptor subtypes. 

Contrasting in vitro and in vivo effects of p-chloroamphetamine (PCA) on 5-HT axons Immunocytochemical studies in hippocampal slices. Abstr Soc Neurosci 14 210, 1988. 

Rosier, O. Joh, T.H. and Beaudet, A. Ultrastmctural relationships between serotonin and dopamine neurons in the rat arcuate nucleus and medial zona incerta A combined radioautographic and immunocytochemical study. Neurosci Lett 48 279-285, 1984. 

Giannetti N., Pellier V., Oestreicher A.B. and Astic L. (1995). Immunocytochemical study of the differentiation process of the Septal Organ of Masera in developing rats. Dev Brain Res 84, 287-293. 

Takagi, H., Morishima, Y., Matsuyama, T. el al (1986). Histaminergic axons in the neostriatum and cerebral cortex of the rat a correlated light and electron microscopic immunocytochemical study using histidine decarboxylase as a marker. Brain Res. 364, 114-23. 

Vos, J.G., Ruitenberg, E.J., Van Basten, N., Buys, J., Elgersma, A. and Kruizinga, W. (1983) The athymic nude rat. IV. Immunocytochemical study to detect T-cells, and immunological and histopathological reactions against Trichinella spiralis. Parasite Immunology 5, 195—215. 

Fellowes, R.A., Dougan, P.M., Maule, A.G., Marks, N.J. and Halton, D.W. (1999) Neuromusculature of the ovijector of Ascaris suum (Ascaroidea, Nematoda) an ultrastructural and immunocytochemical study. Journal of Comparative Neurology 415, 518-528. 

Sithigorngul, P., Stretton, A.O.W. and Cowden, C. (1990) Neuropeptide diversity in Ascaris an immunocytochemical study. Journal of Comparative Neurology 294, 362-376. 

Smart, D.S., Johnston, C.F., Maule, A.G., Halton, D.W., Hrckova, G., Shaw, C. and Buchanan, K.D. (1995) Localization of Diploptera punctata allatostatin-like immunoreactivity in helminths an immunocytochemical study. Parasitology 110, 87-96. 

Immunocytochemical studies of GABAa receptor subunit localization have revealed that oq, p2, and p3 subunits are equally distributed throughout the six cortical layers, whereas oq, a4, 72, and 8 are enriched in the outermost layers. In contrast, a3, oq, and Pi subunits are concentrated in the inner layers (a3 predominantly in layer V-VI, oq in IV-VI, and pi in IV) (36). 

It is at this point that the real fun begins, to visualize the material and determine whether the obtained results are scientifically useful. Instrument accessibility is an important aspect of EM immunocytochemical studies. EM facilities are expensive to acquire and maintain, which induces many universities and other organizations to centralize the EMS and require advance scheduling. This can impede developing an immunochemical assay where multiple samples must be examined in order to refine an assay protocol. It may be necessary to repeat the assays many times before obtaining a satisfactory result. It is very useful to have access to an EM that allows an assay to be conducted, followed by a quick examination of the grid, and then to conduct additional rounds of assays and examination. In most cases, it will take several assays before... 

Naftolin F, Horvath TL, Jakab RL, Leranth C, Harada N, et al. 1996. Aromatase immunoreactivity in axon terminals of the vertebrate brain. An immunocytochemical study on quail, rat, monkey and human tissues. Neuroendocrinology... 

Couce M, Weatherington A, McGinty JE. 1992. Expression of insulin-like growth factor-II (IGF-II) and IGE-II/Mannose-6-phosphate receptor in the rat hippocampus an in situ hybridization and immunocytochemical study. Endocrinology 131 1636-1642. 

Liners, F., Van Cutsem, P. (1992). Distribution of pectic polysaccharides throughout walls of suspension-cultured carrot cells - An immunocytochemical study. Protoplasma, 170,10-21. 

Scheffer, R. C. T., Poort, C. and Slot, J. W. 1980. Fate of the major zymogen granule membrane-associated glycoproteins from rat pancreas. A biochemical and immunocytochemical study. Eur. J. Cell Biol 23, 122-128. 

Sako, H., Nakane, Y, Okino, K., Nishihara, K., Kodama, M., Paku, K., Takayama, H, Tomoyoshi, T, Kawata, M, and Yamada, H (1987) Immunocytochemical study of the cells infiltrating human renal allografts by the ABC and the IGSS methods using monoclonal antibodies. Transplantation 44, 43-50... 

Robertson, D, Monaghan, P., Clark, C., and Atherton, A. J (1992) An appraisal of low temperature embedding by progressive lowering of temperature into Lowicryl HM20 for immunocytochemical studies J Microsc. 168, 85—100... 

Gustafsson, M.K., Terenina, N.B., Reuter, M. and Movsessian, S.O. (2003) NO nerves and their targets in a tapeworm an immunocytochemical study of cGMP in Flymenolepis diminuta. Parasitology Research 90, 148-152. 

Solis-Soto, J.M. and Brink, M.D. (1994) Immunocytochemical study on biologically active neurosubstances in daughter sporocysts and cercaria of Trichobilharzia ocellata and Schistosoma mansoni. Parasitology 108, 301-31 1. 

Terenina, N.B. and Custafsson, M.K. (2003) Nitric oxide and its target cells in cercaria of Diplostomum chro-matophorum a histochemical and immunocytochemical study. Parasitology Research 89, 199-206. 

Paridaens, D. A., Alexander, R. A., Hungerford, J. L., and McCartney., A. C. E. 1991. Oestrogen receptors in conjunctival malignant melanoma Immunocytochemical study using formalin-fixed paraffin wax sections. J. Clin. Pathol. 44 840-843. 

Vorbrodt, A.W., et al. 1995. Immunocytochemical studies of protamine-induced blood-brain barrier opening to endogenous albumin. Acta Neuropathol (Berl) 89 491. 

Siaud P, Manzoni O, Balmefrezol M, Barbanel G, Assenmacher I, Alonso G. The organization of prolactin-like-immunoreactive neurons in the rat central nervous system, light- and electron-microscopic immunocytochemical studies. Cell Tissue Res 1989 255 107-115. 

J.M. Lauder, A.C. Towle, K. Patrick, P. Henderson and H. Krebs, Decreased serotonin content of embryonic raphe neurons following maternal administration of p-chlorophenylalanine a quantitative immunocytochemical study, Develop. Brain Res., 20 (1985) 107-114. 

A14. Alexander, E., Buyon, I. P., Provost, T. T., and Guamieri, T., Anti-Ro/SS-A antibodies in the pathophysiology of congenital heart block in neonatal lupus syndrome, an experimental model. In vitro evectrophysiologic and immunocytochemical studies. Arthritis Rheum. 35, 176—189 (1992). 

Wright R. and Rine J. (1989) Transmission electron microscopy and immunocytochemical studies of yeast analysis of HMG-Co A reductase overproduction by electron microscopy. Met. Cell. Biol. 31, 473-512. 

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