Diploptera punctata

Smart, D.S., Johnston, C.F., Maule, A.G., Halton, D.W., Hrckova, G., Shaw, C. and Buchanan, K.D. (1995) Localization of Diploptera punctata allatostatin-like immunoreactivity in helminths an immunocytochemical study. Parasitology 110, 87-96. 

Some cockroach species exhibit obligatory or facultative parthenogenesis, but most species reproduce sexually with reproductive modes that include oviparity, ovo-viviparity, and viviparity (Roth, 1970). Oviparous females oviposit their eggs inside a protective egg case (ootheca), which is either deposited soon after being produced or carried by the mother until the nymphs hatch. In ovoviviparous and viviparous species, the egg case is reduced and remains unsclerotized it is incubated within a brood sac or uterus and live nymphs hatch from the female. At least one species, Diploptera punctata (Eschscholtz), is viviparous and the embryos receive nutrients directly from the mother in the form of milk (Evans and Stay, 1995). 

Evans, L. D. and Stay, B. (1995). Regulation of competence for milk production in Diploptera punctata interaction between mating, ovaries and the corpus allatum. Invertebrate Reproduction and Development 28 161-170. 

Mundall E. C., Szibbo C. M. and Tobe S. S. (1983) Vitellogenin induced in adult male Diploptera punctata by juvenile hormone and juvenile hormone analogue identification and quantitative aspects. J. Insect Physiol. 29, 201-207. 

Youngsteadt, E., Fan, Y., Stay, B. and Schal, C. (2005). Cuticular hydrocarbon synthesis and its maternal provisioning to embryos in the viviparous cockroach Diploptera punctata. J. Insect Physiol., 51, 803-809. 

Figure 2. HPLC of precocene II cis- and trans-3,4-dlhydrodlols obtained from iii vitro incubation of Diploptera punctata corpora allata with H-precocene II.

Figure 4. Effect of various Inhibitors on JH 111 biosynthesis by Diploptera punctata corpora allata In vitro ...

Figure 6. Inhibition of oocyte growth in adult female Diploptera punctata over a 3-day period (day 2 to day 5).

Neuropeptide Inhibitors of Juvenile Hormone Synthesis in Brain and Corpora Allata of the Cockroach Diploptera punctata... 

The allatostatins are a family of brain peptides which rapidly and reversibly inhibit juvenile hormone biosynthesis in isolated insect corpora allata. Five allatostatic peptides from brains of the cockroach Diploptera punctata have now been structurally identified (i 2 our unpublished results), their size ranging from octapeptide to octadecapeptide. The currently identified allatostatins all possess the consensus C-terminal sequence (L/V)YXFGL-amide, which has no significant homology with other known peptides. It is likely that they are released locally from nerve terminals within the glands (3). 

Allatostatic peptides from brains of Diploptera punctata biological activity, 188,189/ control of sample loss, 178-179 dose-response curves for biological activity, 188,189/ effect of N-terminal truncation on activity, 191-192... 

Fig. (2). Rates of juvenile hormone III (JH III) biosynthesis by the corpora aiiata (CA) of the penuitimnte and final instars of the cockroach, Diploptera punctata [43]. The rate of biosynthesis, which is closely correlated to haemolymph titers, was measured by an in vitro JH III radiochemical assay [45, 46, 47], Each point represents the mean of 3-12 determinations. Arrows indicate approximate times of ecdysis (reprinted with kind permission from Academic Press).

Fig. (4). Jnvenile hormone III (JH III) haemolymph litres in mated adult females of the viviparous cockroach, Diploptera punctata [57], Haemolymph litre was measured by derivitizing JH III and subjecting the resultant methoxyhydrin to gas chromatography-mass spectroscopy [37], Arrows indicate approximate time of oviposition (reprinted with kind permission from Birkhaeuser Publ.).

Fig. (5). Biosynthetic pathway of juvenile hormone III in the cockroach, Diploptera punctata [65, 71].

Allatostatins (AST), a family of neuropeptides first isolated from the brain of the cockroach Diploptera punctata. There are three allotostatin families according to C-terminal sequence homology (a) the cockroach type (>70 peptides, consensus sequence FGLa), comprising... 

C7 Diploptera punctata Farnesol, farnesal, famesoic acid, methyl farneso- [251] ... 

The members in this class of reproductive and developmental neuropeptides have been isolated and sequenced from M. sexta and the cockroach, Diploptera punctata. The allatotropin (AT) from Af. sexta (see Table I) is the only AT to be isolated and sequenced (775). This 13-residue peptide was isolated from 10,000... 

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