Immunization guinea pig

Jemski, J. (1961b). Challenge of parenterally immunized guinea pigs with aerosols of botulinum toxins disseminated by the Hartman fixture at 75°F and 50% relative humidity. Fort Detrick Applied Aerobiology Division - Technical Evaluation Report Test No. 60-TE-1323. DTIC AD0497563. 

LAPA E SILVA, J.R., BACHELET, C.M., PRETOLANI, M BAKER, D SCHEPER, R.J. VARGAFTIG, B.B. (1993) Immunopathologic alterations in the bronchi of immunized guinea pigs. American Journal of Respiratory Cell and Molecular Biology, 9, 44—53. 

Rosenstreich DL, Blake JT, Rosenthal AS (1971) The peritoneal exudate lymphocytes. I. Differences in antigen responsiveness between peritoneal exudate and lymph node lymphocytes from immunized guinea pigs. J Exp Med 134 1170-1186... 

Liu M, Wang L, Enhoming G. Surfactant dysfunction develops when the immunized guinea pig is challenged with ovalbumin aerosol. Clin Exp Allergy 1995 25 1053-1060. 

PH-20, a guinea pig sperm protein of 64 kD, is present on both the plasma membrane and inner acrosomal membrane of sperm. It is essential for adhesion of sperm to the zona peUucida, the initial step in the fertilization process. Active immunization with PH-20 causes infertility in both male and female guinea pigs for a period ranging from 6 to 15 months (120). 

Guinea pig, Cavia 50 or 150 mg/kg diet No evidence of altered immune function 11... 

Vos, J.G. and de Roij, T., Immunosuppressive activity of a polychlorinated diphenyl preparation on the humoral immune response in guinea pigs, Toxicol. Appl. Pharmacol., 21, 549, 1972. 

Katsutani, N., and Shionoya, H., Drug-specific immune responses induced by immunization with drugs in guinea pigs and mice. J. Toxicol. Sci., 17, 169, 1992. 

Cell-mediated immunity Cytotoxic T lymphocyte killing delayed type hypersensitivity response mouse ear swelling test (MEST Gad et al., 1986) guinea pig maximization test (Magnusson and Kligman, 1969). 

Ueda, S., Wakahim, Y., Takei, L, Mori, T. and Lesato, K. (1980). Autologous immune complex nephritis in gold injected guinea pigs. Nippon Jinzo Gakkai Shi 22 1221-1230. 

The guinea pig maximization test (GPMT) is a preferred method for the detection of skin sensitizers. It belongs to the class of adjuvant-tests, where the substance will be applied in Freund s complete adjuvant (FCA). The test is based on the possible induction of an immune response of the skin during an induction period (at least 1 week). This pretreatment of the subject will eventually result in a hypersensitive reaction during a further exposure, the so-called challenging phase. 

The test is started with an intradermal and/or epidermal application of the test substance, using the induction dose on young adult guinea pigs of either gender. In the case of female animals, these have to be nulliparous and nonpregnant. For 5 days prior to the test, animals are kept at (20 3)°C with 30-70% relative humidity, conventional laboratory diet, and unlimited access to water. The induction dose is administered to the shoulder region and is followed by the induction period (10-14 days), in which the animals can rest and a possible immune response may develop. It should be noted that the location of the induction dose is kept occluded for the first 48 h after administration. 

Tolo K (1974) Penetration of human albumin through the oral mucosa of guinea-pigs immunized to this protein. Arch Oral Biol 19 259-263... 

In a review. Arts and Kuper (2007) have summarized the animal test methods, which have been used to detect immune-mediated respiratory disease. The tests for respiratory sensitization include dermal as well as inhalatory or topical exposure of mice, rats, or guinea pigs for induction and challenge, and may measure various endpoints to evaluate respiratory sensitization. The review concludes that standardized and validated dose-response test methods are urgently required in order to allow identification of respiratory allergens and to make it possible to recommend safe exposure levels for consumers and workers. 

There are a number of models for detecting allergic contact dermatitis in guinea pigs. The maximisation test developed by Magnussun and Kligman is the most widely used and employs both an intradermal and topical sensitisation phase, together with the non-specific stimulation of the immune system by the intradermal injection of Freund s complete adjuvant. 

No histopathological effects in the lymph nodes were found in rats, guinea pigs, rabbits, or dogs exposed to 5 mg thorium/m (0.550 nCi/m = 20 Bq/m ) for 1 year as thorium dioxide (Hodge et al. 1960). Since no parameters of immune function were examined, this value does not appear as a NOAEL for immunological effects in Table 2-1 or Figure 2-1. 

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