Human cancer cell lines melanoma

Haterumadioxins A and B were isolated from the Okinawan sponge Plakortis lita. Haterumadioxin A 100 was evaluated against a human cancer cell line panel and found to be effective against a melanoma cancer cell line. An analysis of the results obtained... 

In a study of the cytotoxic effects of the alkaloids of M. euchrestifolia on human cancer cell lines, it was found that while the monomeric alkaloids murrayaquinone and murrayafoline showed significant cytotoxicity against SK-MEL-5 and Colo-205 cells, the biscarbazole alkaloids, bismurrayafoline A (21) and chrestifoline A (40) by comparison, showed only marginal cytotoxicity against HOP-92, a nonsmall cell lung cancer cell line, and LOXIMVI, a melanoma cell line, whereas chrestifoline C (42), bismurrayafoline B (22), and murranimbine (48), were inactive toward all the cell lines tested (40). 

Synoxazolidinone C (3) also exhibited eytotoxic activity against the human cancer cell line s A2058 (melanoma ), MCF-7 (breast adenocarcinoma ) and HT-29 (colon carcinoma ) with an IC50 value of 30.5 gM. It was also found that at the same level of concentration normal lung fibroblast cells (MRC-5) were killed. 

In vitro, EGCG has been shown to cause growth inhibition and apoptosis in a number of human cancer cell lines including leukemia, melanoma, breast cancer, lung, bladder, and colon [47, 287-289]. Yamauchi (2009) reported EGCG in green tea polyphenols (GTP) as a potent apoptosis inducer that functions exclusively... 

Current efforts favor tumor cell line tests, conducted by the National Cancer Institute (NCI) drug development program [62]. In the current NCI anticancer screen, each compound is tested against 60 human tumor cell lines derived from several cancer types (lung, colon, melanoma, kidney, breast, ovary, brain, leukemia). The tumor cells are seeded on 96-well microtiter plates and pre-incubated for 24 h. The test agents are then added to the wells (five 10-fold dilutions 0.01 -100 pmol/1) and are incubated for 48 h with the tumor cell lines. At the termination of the assay, the cells are fixed in situ with trichloroacetic acid (TCA), washed and dried. Sulforhodamine B (SRB), a dye that binds to the basic amino... 

Preliminary in vitro and animal studies of the effects of silymarin and silybinin have been carried out with several cancer cell lines. In murine models of skin cancer, silybinin and silymarin were said to reduce tumor initiation and promotion. Induction of apoptosis has also been reported using silymarin in a variety of malignant human cell lines (eg, melanoma, prostate, leukemia cells, bladder transitional-cell papilloma cells, and hepatoma cells). Inhibition of cell growth and proliferation by inducing a Gx cell cycle arrest has also been claimed in cultured human breast and prostate cancer cell lines. The use of milk thistle in the clinical treatment of cancer has not yet been adequately studied but preliminary trials are under way. 

Astragaloside II, a cycloartane triterpene glycoside isolated from the Egyptian Astragalus spinosus, was tested at minimum of five concentrations 10-fold dilution against a total of 60 human tumor cell lines derived from seven cancer types (lung, colon, melanoma, renal, ovarian, brain and leukemia). The results indicated that the colon cancer (SW-620) and the leukemias (CCRF-CEM, HL-60) were the most sensitive cell lines [98]... 

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