HMSCs cells

Stem cell isolation and expansion BM aspirates were combined with DPBS and centrifuged several times at different density gradients to collect hMSCs. Cells were then resuspended in hMSC complete culture medium and plated at 3 x 107 cells/185 cm2. The cultures were maintained at 37°C in a humidified atmosphere before being passaged prior to confluency. 643694"... 

Another study looked at the relationship between selected markers and hMSC-mediated immunosuppression in vitro. It was found that co-culture of hMSC with anti-CD3/CD28 activated peripheral blood mononuclear cells (hPBMCs) caused inhibition of lymphocyte proliferation. The effect of hMSCs on lymphocyte proliferation was dose dependent, causing more than 50% inhibition at an approximately 1 10 to 1 25 MSC lymphocyte ratio. Five-day... 

In October 2004, clinical data from a phase I safety trial of OTI-OIO therapy for the treatment of GVHD were reported. Infusion of hMSCs decreased GVHD in 22 to 45% of patients, suggesting that OTI-OIO reduces the numbers of pro-inflammatoiy cytokines and T-cells while increasing levels of anti-inflammatory cytokines in these patients [570810]. 

In vitro Modulation of allogeneic immune cell responses by hMSC hMSCs incubated with DCs and naive T-cells When incubated with DC a > 50% decrease in TNFa secretion and a > 50% increase in IL-10 secretion were observed. hMSC decreased IFN. Secretion by Thl and NK cells and increased IL-4 secretion by Th2 cells when incubated with naive T-cells 643694"... 

In vivo HMSC engraftment and migration Fischer rat MSCs were infused intraportally into the livers of other Fischer rats (2 million cells/rat) Results showed cells to persist for at least 28 days, being localized to the portal triad regions 643859... 

In this study, the authors examined the immunomodulary functions of human MSCs by coculturing them with purified subpopulations of immune cells, and report that hMSCs alter the cytokine secretion profile of the host immune cells to induce a more antiinflammatory phenotype. The authors discuss and propose mechanisms underlying the immunomodulaiy functions of hMSCs in allogeneic transplantation. A shift from a proinflammatory environment toward an anti-inflammatory or tolerant cell environment would result in inflammation modulation, tolerance induction and reduction of allogeneic transplantation complications. 

Substrate topographical patterns have also been shown to affect the differentiation of MSCs to nerve cells. Yim et al. investigated the differentiation and proliferation of hMSCs on nanogratings of 350 nm width and formd alignment and elongation of... 

PGA, PDLLA, PLLA, PLGA, PCL Nonwoven scaffold TE SEM, tensile test, degradation evaluation, in vitro chondrocytes and hMSCs culture, cell proliferation analysis (Li et al. 2006)... 

FIGURE 11.10 Permeation of hMSCs on (a) nonfunctionalized PCL and (b) PCL electrospun fibers functionalized by a 2% solution of PNEAmHi. Cells were allowed to culture for 4 weeks to obsawe cell infiltration. Functionalization promoted cell infiltration from the scaffold surface. Scaffold seeded at 30,000 cells per 1 x 1 cm scaffold. Scale bars=1000pm. 

S. Neuss, et al.. Long-term survival and bipotent terminal differentiation of human mesenchymal stem cells (hMSC) in combination with a commercially available three-dimensional collagen scaffold. Cell Transplant. 17 (2008) 977-986. 

C.B. Kwok, F.C. Ho, C.W. Li, A.H.W. Ngan, D. Chan, B.P. Chan, Compression-induced alignment and elongation of human mesenchymal stem cell (hMSC) in 3D collagen constructs is collagen concentration dependent, J. Biomed. Mater. Res. lOlA (6) (2013) 1716-1725. 

Spherical particles were examined for their cellular uptake characteristics by FITC-based flow cytometry and in vitro MRI. In vivo MRI was recorded in a nude mouse model where Gd-Dye MSN-IabeIed human mesenchymal stem cells (HMSCs) were implanted into the basal ganglia of the mice and observed over a 14 day period by 1.5 T MRI Tj-weighted imaging. The cells remained visible as bright dots for that period with no evidence of cell migration. Furthermore, in related... 

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