High-performance liquid chromatography anion exchange

Torres, A. R., Edberg, S.C., and Peterson, E. A., Preparative high-performance liquid chromatography of proteins on an anion exchanger using unfractionated carboxymethyl displacers, /. Chromatogr., 389, 177, 1987. 

Dizdaroglu, M., Krutzsch, H. C., and Simic, M. G., Separation of angiotensins by high-performance liquid chromatography on a weak anion-exchange bonded phase, Anal. Biochem., 123, 190, 1982. 

Rounds, M.A. and Nielsen, S. S., Anion-exchange high-performance liquid chromatography with post-column detection for the analysis of phytic acid and other inositol phosphates, /. Chromatogr. A, 653, 148, 1993. 

Despite its potential importance, formic acid has proven difficult to quantify at submicromolar levels in non-saline water samples. Formidable analytical difficulties are associated with its detection in highly saline samples. Ion exclusion, anion exchange, and reversed-phase high performance liquid chromatography techniques based on the direct detection of formic acid in aqueous samples are prone to interferences (especially from inorganic salts) that ultimately limit the sensitivity of these methods. 

Note. Abbreviations g.l.c., gas-liquid chromatography e.i.-m.s., electron-impact mass spectrometry c.i.-m.s., chemical-ionization mass spectrometry h.p.l.c., high-performance liquid chromatography h.p.a.e., high pH anion-exchange f.a.b.-m.s., fast-atom-bombardment mass spectrometry l.s.i.-m.s., liquid secondary-ion mass spectrometry n.O.e., nuclear Overhauser enhancement. Details of these methodologies are given in Ref. (3). 

Nogueira, R., Lubda, D., Leitner, A., Bicker, W, Maier, N.M., Lammerhofer, M., and Lindner, W, Silica-based monohthic columns with mixed-mode reversed-phase/weak anion-exchange selectivity principle for high-performance liquid chromatography, J. Sep. ScL, 29, 966, 2006. 

Ishii, D., Hirose, A., and Horiuchi, I., Studies on micro-high-performance liquid-chromatography. 6.Application of microscale liquid-chromatographic technique to anion-exchange separation of halide ions. Journal of Radioanalytical Chemistry A5( ), 7-14, 1978. 

Qiu, H., Jiang, S., and Liu, X., N-Methylimidazolium anion-exchange stationary phase for high-performance liquid chromatography, /. Chromatogr. A, 1103, 265-270,2006. 

Mancinelli, V., M. Rinaldi, E. Finessi, et al. 2007. An anion-exchange high-performance liquid chromatography method coupled to total organic carbon determination for the analysis of water-soluble organic aerosols./. Chromatogr. A 1149 385-389. 

Arsenic species were preconcentrated on Zipax, a pellicular anion exchange material and separated on a column packed with high performance liquid chromatography grade strong anion exchange resin, then continuously reduced with sodium tetrahydroborate and detected by atomic absorption spectrometiy. Detection limits were 2ng for arsenite, arsenate and monomethylarsinate and lng for dimethylarsonate. 

Takeuchi et al. [343] described an indirect photometric detection method for anions in micro high performance liquid chromatography. Micro anion exchange octadecylsilica columns permanently coated with the hydrophobic cetyltrimethylammonium ion (cetrimide) were used in the high performance liquid chromatography of chloride, nitrate... 

Takana et al. [344] used high performance liquid chromatography on an anion exchange column with differential pulse polarographic detection to determine thiosulphate and tri, tetra-, penta- and hexathionates in trade effluents. The method is accurate to within 10% at the 0,001 1 mM concentration range. 

Apparatus Use a suitable high-performance liquid chromatography system (see Chromatography, Appendix IIA) equipped with a dual wavelength detector system such that the effluent can be monitored serially at 254 nm and 325 to 385 nm (wide-band pass). Use a 1-m x 2.1-mm (id) column, or equivalent, packed with a strong anion-exchange resin (Dupont No. 830950405, or equivalent). 

M. Lammerhofer and W. Lindner, Quinince and quinidine derivatives as chiral selectors. Beush type chiral stationary phases for high performance liquid chromatography based on chincona carbamates and their applications as chiral anion exchanger, J. Chromatogr. 741 (1966), 33. 

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