Anion exchange separations

Fig. 5. Anion-exchange separation of insulin and insulin A- and B-chains, over diethylaminoethyl (DEAF) in a 10.9 x 200 mm column having a volume of 18.7 mL. Sample volume is 0.5 mL and protein concentration ia 16.7 mAf Tris buffer at pH 7.3 is 1 mg/mL for each component ia the presence of EDTA. Eluent (also 16.7 mAf Tris buffer, pH 7.3) flow rate is 1.27 ml,/min, and protein detection is by uv absorbance at 280 nm. The straight line depicts the salt...

Lovdahl, M. J. and Pietrzyk, D. J., Anion-exchange separation and determination of bisphosphonates and related analytes by post-column indirect fluorescence detection,. Chromatogr A, 850, 143, 1999. 

FIGURE 8.12 UV absorbance chromatograms of three anion-exchange separations of an E. coli lysate. Vertical lines represent the times at which fractions were changed. 

Figure 5.14. Elution curves of the rare earth elements in interfering elements for anion exchange separation using CH3COOH/HNO3 (1st columns) and MeOH/HNCh (2nd columns) mixtures. Source [629]...

Figure 8 Anion exchange separation of diethanolamine (DEA), glycine, hydroxyethyl glycine (HEG), iminodiacetic acid (IDA), and glyphosate followed by ECL detection. Mobile phase consisting of 0.01 mM Ru(bpy)32+ in 10% acetonitrile, 90% 0.01 M phosphate buffer at pH 9.8. (Reprinted from Ref. 61, with permission from, and copyright, Elsevier Science.)...

Figure 4.7 Anion exchange separation of carboxylic acids in red wine. Column, Shodex C811, 100 cm x 7.6 mm i.d. eluent, 3 mM perchloric acid flow rate, 0.9 ml min-1 temperature, 60 °C detection, reaction detection using chloro-phenol red at 430 nm. Peaks 1, citric acid 2, tartaric acid 3, malic acid 4, succinic acid 5, lactic acid 6, formic acid and 1, acetic acid.

Ishii, D., Hirose, A., and Horiuchi, I., Studies on micro-high-performance liquid-chromatography. 6.Application of microscale liquid-chromatographic technique to anion-exchange separation of halide ions. Journal of Radioanalytical Chemistry A5( ), 7-14, 1978. 

Historically, the first suppressor was a packed-column in the H+ or OH" form. Introduced in 1975 [1], it actually represented the foundation on which the IC was built. To depict the reactions taking place during the suppression for an anion-exchange separation, the following equilibria must be considered ... 

Pharmacia s Q- and S-Sepharose anion- and cation-exchange resins For the anion-exchange process it was found that two step changes, simultaneous in pH and salt concentration were necessary to carry out the anion-exchange separation. A 0.01 M sodium acetate buffer, pH 5.8, was used forthe starting state orfeed loading buffer. After the whey feed was loaded onto the column, one column volume of this... 

Scheme 26. Proposed synthesis of polymeric catenane 11, with anion-exchange separation of cyclic species from linear polymers [33].

Fig. 2. Anion exchange separation of trace anions in HF following fluoride removal using ion exclusion. See text for details. Peaks 1, fluoride 2, chloride, 7.9 (Xg/1 3, carbonate 4, nitrate, 0.89 mg/1 5, unidentified 6, sulfate 10.1 mg/1 7, phosphate, 2.4 jxg/1.

U. B. Larsson, I. Norstedt, and O. Samuelson, Anion exchange separation of organic acids in acetate medium influence of temperature, J. Chromatogr., 22 (1966) 102-109. 

Woodruff A., Pohl, C. A., Bordunov, A., and Avdalovic, N. (2003) Environmental Applications of a Cryptand Adjustable-capacity Anion-exchange Separator, J. Chromatogr. A 997, 33-39. 

Good, M.L., Purdy, M.B. and Hoering, T. (1958) The anion-exchange separation of iodine anions./. Inorg. Nucl. Chem., 6, 73-75. 

Cavallie, S. and Cardellichio, N. (1995) Direct determination of selenoamino acids in biological tissues by anion-exchange separation and electrochemical detection. J. Chromatogr. A, 706, 429-436. 

Figure 3 Analysis of selected fractions from DEAE column by 2-D PAGE. Fractions m, IV, and V from the anion-exchange separation of rat serum (Fig. 2) were analyzed by 2-D PAGE. Low-abundance proteins enriched by fractionation are noted with arrowheads.

Column chromatography [50] has been used to determine sulphate in waste water. A post-column solid phase reaction detector was employed in conjunction with an anion exchange separation column to determine 5 to 40mg L 1 sulphate. 

The polymer derivative was shown to be applicable to anion exchange separation of a number of anions. 

Fig. 2. Anion exchange separation of trimethylammonium polyfethy-leneimine) Retention as a function of the filtration factor Z (ratio of filtrate volume to cell volume) (4% w/w, 0.01 M sodium salts, pH 8.5) [33]...

Complex formation is studied experimentally by liquid-liquid extractions, or anion exchange separations. For a simple complex MLn, the cumulative complex formation constant... 

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