High performance anion exchange

High performance anion-exchange chromatography evidence that the synthesized product is homogalacturonan... 

High-performance anion-exchange chromatography (HPAEC) of the reaction products from MHR-S showed that RGaseA and B acted differently towards this substrate (Fig. 1). 

Fig. 7. High-performance anion-exchange chromatography of the reaction products from alkali saponified SPS, produced by the crude A. acukatns preparation (Ultra-SP) and the partially purified fraction HTP-2.

Lee, YC (1990) High-performance anion-exchange chromatography for carbohydrate analysis. AnalBiochem 189 151-162... 

Figure 6A-C. High-performance anion-exchange chromatography elution profile of isolated modified hairy regions without addition of enzymes (A), with Biopectinase OS (B) and with rhamnogalacturonase-containing culture filtrate from an A. awamori multicopy transformant (C). /xC ftCoulomb- Data taken from Ref. 6.

Hutchens, T. W., Li, C. M., and Paige, P. K., Performance evaluation of a focusing buffer developed for chromatofocusing on high-performance anion-exchange columns, /. Chromatogr., 359, 169, 1986. 

Alonso, ]., Nogues, M. V., and Cuchillo, C. M. Separation of RNA derivatives by high-performance anion-exchange liquid chromatography, J. Liq. Chromatogr., 8, 299, 1985. 

Drager, R. R. and Regnier, F. E., High-performance anion-exchange chromatography of oligonucleotides, Anal. Biochem., 145, 47, 1985. 

De Ruiter, G. A., Schols, H. A., Voragen, A. G. J., and Rombouts, F. M., Carbohydrate analysis of water-soluble uronic acid-containing polysaccharides with high-performance anion-exchange chromatography using methanolysis combined with TFA hydrolysis is superior to four other methods, Anal. Biochem., 207, 176, 1992. 

Martens, D.A. and Frankenberger, Jr., W. T., Determination of saccharides by high-performance anion-exchange chromatography with pulsed amperometric detection, Chromatographia, 29, 7, 1990. 

Paskach, T. J., Lieker, H.-R, Reilly, P. J., and Thielecke, K., High-performance anion-exchange chromatography of sugars and sugar alcohols on quaternary ammonium resins under alkaline conditions, Carb. Res., 215,1, 1991. 

Watson, E., Bhide, A., Kenney, W. C., and Lin, F.-K., High-performance anion-exchange chromatography of asparagine-linked oligosaccharides, Anal. Bio-chem., 205, 90, 1992. 

Hernandez, L. M., Ballou, L., and Ballou, C. E., Separation of yeast asparagine-linked oligosaccharides by high-performance anion-exchange chromatography, Carb. Res., 203, 1, 1990. 

Lin, S. L., Inoue, Y., and Inoue S., Evaluation of high-performance anion-exchange chromatography with pulsed electrochemical and fluorometric detection for extensive application to the analysis of homologous series of oligo-and polysialic acids in bioactive molecules, Glycobiology, 9, 807, 1999. 

Conboy, J. J. Henion, J. High performance anion exchange chromatography coupled with mass spectrometry for the determination of carbohydrates. Biol. Mass Spectrom. 1992, 21, 397—407. 

Bao YD, Silva TJ, Guerrant RL, Lima AM, Fox JW Direct analysis of mannitol, lactulose and glucose in urine samples by high-performance anion-exchange chromatography with pulse amperometric detection - clinical evaluation of intestinal permeability in human immunodeficiency virus infection. J Chromatogr 1996 685 105-112. 

Ion chromatography has become available and is used for sugar analysis. In this system a high-performance anion exchange column is used at high pH. This separation works because neutral saccharides behave as weak acids. Table 1 shows some pKa values. 

JOL C N, NEIESS T G, PENNINKHOF B, RUDOLPH B and DE RUITER G A (1999), A novel high-performance anion-exchange chromatographic method for the... 

Kaiser, K., and R. Benner. 2000. Determination of amino sugars in environmental samples with high salt content by high performance anion exchange chromatography and pulsed amperometric detection. Analytical Chemistry 72(ll) 2566-2572. 

Field M., Papac D., and Jones A. (1996), The use of high-performance anion-exchange chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to monitor and identify oligosaccharide degradation, Anal. Biochem. 239, 92-98. 

Two anion exchange columns Dionex CarboPac PA1 plus a guard column and CarboPac MAI column with a guard column were used for separation procedure (High Performance Anion Exchange Chromatography). 

---