Hewlett-Packard liquid chromatograph

Samples were analyzed using an SEC system consisting of a Hewlett Packard liquid chromatograph 1090 and a Wyatt interferometric refiactometer detector. The mobile phase was toluene pumped at a flow rate of 1 mL/min. 100 pL of sample solution (5 mg/mL) were injected. The stationary phase consisted of 3 columns (300 mm x 8 mm ID) filled with PSS SDV gel (10 , 10, 10 A 5 pm). The software of PSS Mainz (WIN GPC) was used for data acquisition. 

Chemical purities were determined by HPLC on a Hewlett-Packard liquid chromatograph HP1090 series II using a LichroCART 125-4 Superspher 60-RP Select B column (Merck) and UV-detection at 254 nm wavelength. Separations were achieved with a linear gradient of Ig H3PO4 85% in 100 mL water and Ig H3PO4 85 % in 100 mL acetonitrile as mobile phase at a 1.2 mL/min. flow. 

Hydrotreating was carried out at 3.1 MPa (450 psig) and 613 K (340 °C) in a three-phase upflow fixed-bed reactor (Figure 1). The feed liquid was prepared by combining different quantities of 1.0 wt% tetralin (Aldrich, 99%), 0.02 wt% N as quinoline (Aldrich, 99%), 0.05 wt% S as 4,6-dimethyldibenzothiophene (4,6-DMDBT, Fisher, 95%), 0.3 wt% S as dimethyldisulfide (DMDS, Aldrich, 99%), n-octane (Aldrich, 99%), and balance n-tridecane (Alfa Aesar, 99%). Liquid product compositions were determined with a Hewlett-Packard 5890A chromatograph equipped with a 50 m dimethylsiloxane colunm (Chrompack, CPSil 5B) of 0.32 mm i.d. Reaction products were identified by matching retention times with commercially available standards. 

High-performance liquid chromatography is performed using a Hewlett-Packard 1090 chromatograph equipped with a ternary-solvent delivery system, an autoinjector with a 0 -20- u.L injection loop, an oven compartment, and a diode-array UV detector. An ELS detector (Alltech Associates, Deerfield, IL) is connected in series to the UV detector. Hexane, 2-propanol, and water were used for the analysis of nonionic surfactants. Water and tetrahydrofuran (THF) are used for the analysis of anionic surfactants. No preliminary sample preparation is used other than dilution. The nonionic surfactants are diluted 1 40 (v/v) with hexane. The anionic surfactants (alkyl ether sulfates and synthetic and petroleum sulfonates) are diluted 1 20 (v/v) with water-THF (50 50). The calcium sulfonate surfactants were diluted 1 20 (v/v) with a THF-38% hydrochloric acid solution of pH 1. Hydrochloric add is required to prevent salt precipitation by converting any excess water-insoluble caldum carbonate into water-soluble calcium chloride. All diluted samples are... 

A Hewlett Packard gas chromatograph, HP6890, was used for the analysis of liquid samples. A small quantity of the distillate with the distillate composition was kept in a fridge to be used as a feed solution. 

The purity of 1 and 2 is assessed by analytical gas-liquid chromatography (GC) on a Hewlett-Packard 5890 gas chromatograph equipped with a flame-ionization detector and fitted with a 50 m x 0.2 mm HP-5 fused silica glass capillary column using linear temperature programming from an initial temperature of 150°C for 5 min to a final temperature of 200°C for 10 min at a rate of 5°C/min. 

High-performance liquid HPllOO (Hewlett-Packard) chromatograph... 

Analysis Techniques. The contents of the major breakdown products of xetralin (naphthalene and 1-methyl indan) present in the distillate were determined by gas-liquid chromatography using a Hewlett Packard Series 5750 Research Chromatograph with a 62m x 0.5mm diameter glass capillary SCOT column coated with nonpolar SE 30 liquid phase (see Reference (4 ) for details). 

Instrumentation Hewlett Packard supply the HP 5988A and HP 5987A mass-selective detectors for use with liquid chromatographs. 

The lncos-50 is a relatively low-cost benchtop instrument as opposed to the research grade instruments discussed earlier. The gas chromatography-mass spectrometer transfer lines allow it to be used with either the Hewlett Packard 5890 or the Varian 3400 gas chromatographs. The Incos 50 provides data system control of the gas chromatography and accessories such as autosampler or liquid sample concentration. It can be used with capillary, wide-bore or packed columns. It performs electron ionization or chemical ionization with positive or negative detection. It also accepts desorption or other solids controls. 

The minor (R,S) diastereomer is present in the crude reaction mixture to the extent of approximately 0.9% as determined by capillary gas-liquid chromatographic analysis performed on a Hewlett-Packard 5790A gas chromatograph equipped with a Hewlett-Packard 3390A integrator and HP-1 methylsilicone gum column (25 m x 0.2 mm x 0.33 pm film thickness). The checkers found that HPLC analysis (Zorbax SB-Phenyl column 25 cm x 4.6 mm, 40 60 MeCN/0.1% aqueous phorphoric acid, 1.5 mL/min, 250 nm detection) provided satisfactory resolution of the R,R- and R,S-diastereomers. The minor diastereomer is hardly discernible by 1H NMR (500 MHz) after purification by flash chromatography. 

The liquid products obtained in the batch and semicontinuous experiments were analyzed with a Hewlett-Packard Model 5880 gas chromatograph equipped with two 1/8 in x 10 ft 60/70 mesh Chromasorb 101 columns connected in series. 

Fig. 42 Reversed-phase HPLC profiles of natural (top) and rearranged (bottom) butterfat triacylglycerols as obtained with the light-scattering detector. HPLC conditions Hewlett-Packard Model 1050 liquid chromatograph equipped with a Supelcosil LC-18 column (25 cm X 0.46-cm ID) coupled to a Varex ELSD II light-scattering detector. Solvent linear gradient of 10-90% propanol in acetonitrile at 25°C over a period of 90 min (1 ml/min) recording stopped at 70 min. Peak identification by carbon and double-bond numbers of triacylglycerols.

Fig.3.27. Hewlett-Packard Model 1010B liquid chromatograph.

The gasoline feed was cracked over a fixed bed (4.69g) of the relevant catalyst and liquid and gaseous products were collected in conventional MAT receivers. Gaseous products were analysed on a dual-column Hewlett Packard 5890 gas chromatograph where hydrogen and nitrogen were quantified on a 13X column linked to the TCD detector. Individual hydrocarbons in the number range Cl - C7 were quantified on a 50m alumina-PIOT column carbon linked to an FID detector. The liquid fraction from each test was analysed directly on a FIONA analyser supplied by Analytical Control BV. 

INSTRUMENTATION. A Model 1090 Series M liquid chromatograph (Hewlett Packard, Avondale, PA, USA), equipped with a ternary DR5 solvent delivery system, diode-array detector, ColorPro graphic plotter, and autosampler, was used. The chromatographic system and data evaluation were controlled by Series 79994A Chem Station computer. 

The chromatograms presented in Figures 4 and 8-12 were obtained by using the HPLC unit depicted in Figure 2 Results shown in the other figures were from experiments carried out with Hewlett Packard Model 1090 liquid chromatograph. 

A Hewlett-Packard gas-liquid chromatographic unit, a sophisticated analytical instrument for drug testing. 

The HPLC system consisted of a Hewlett Packard (Avondale, PA, USA) HP1090 Liquid Chromatograph equipped with a heater, a photo diode... 

High Performance Size Exclusion Chromatography. The Hewlett-Packard 1090 liquid chromatograph was used with the HP 1040 diode array or HP 1037A refractive index (and HP 3392 integrator) detectors. A fifty A (5 mm, 300 x 7 mm) Polymer Laboratories PL gel (polystyrene-divinylbenzene copolymer gel) column was used and standards were as described in Chum et al. (13). Tetrahydrofuran solutions of oil and oil fractions were analyzed. 

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