Hemoglobin sensor

He J-L, Zou W, Wang J-X, Zeng L-H, Cao Z, Luo D-M. Tetra-(A -phenylpyrazole)-potphyrin/p-cyclodextrin incorporated CNT film modified hemoglobin sensor. Adv Mato" Res 2011 239-242 2831-4. 

FIGURE 8.5 Multiple-sensor respirometry. Representative calibration traces of PNOS (thin line, left ordinate) and PHSS (thick line, right ordinate) operating simultaneously in PBS, pH 7.3 at 37°C, with 50pM DTPA in a closed chamber respirometer. After NO additions were made, the chamber solution was replaced with fresh buffer, to which Na2S stock solutions were then injected in a stepwise manner. The stable POS signal shown at 2 pM 02 demonstrates that the POS does not respond to NO or H2S. Injections of anoxic buffered NO and H2S stocks are shown with concentrations at arrows, as are additions of Lucina pectinata ferric hemoglobin I (metHb I), which stoichiometrically binds to H2S (after [41]). 

Zhang J, Oyama M (2004) A hydrogen peroxide sensor based on the peroxidases activity of hemoglobin immobilized on gold nanoparticles-modified ITO electrode. Electrochim Acta 50 85-90... 

Yang, W. W., Bai, Y., Li, Y. C. and Sun, C. Q. (2005a), Amperometric nitrite sensor based on hemoglobin/colloidal gold nanoparticles immobilized on a glassy carbon electrode by a titania sol-gel film. Anal. Bioanal. Chem., 382(1) 44-50. 

Rusclano G et al (2008) Raman tweezers as a diagnostic tool of Hemoglobin-related blood disorders. Sensors 8(12) 7818-7832... 

Zhujun, Z. Seitz, W.R. Optical sensor for oxygen based on immobilized hemoglobin. Anal. Chem. 1986, 58, 220-222. 

Carbon monoxide is highly toxic because it has a higher affinity for hemoglobin than does oxygen. CO sensors are therefore of utmost importance for combustion control, fire-alarm systems, and detection of hazardous concentrations of the gas in private and industrial facilities such as tunnels, coal mines, and underground garages. The sensors in current use, in which CO is anodically oxidized or adsorbed onto coated piezoelectrodes, are relatively nonspecific, since H2S, N2O, and NO2 interfere. 

Fig. 94. Schematic and measuring curve of a substrate competition sensor for phenols and aniline using immobilized hemoglobin (Hb). (Redrawn from Scheller et al., 1987c).

Since the sensor signal was linear over the normal range of 2.1-6.9 U/g hemoglobin, and only decreased activities are of clinical relevance, the bienzyme electrode is suitable for the measurement of any clinically possible value. 

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