Binding stoichiometric

All the direct metal ligands in Con A are retained in the lectin from favin,360 which also binds stoichiometric amounts of Mn2+ and Ca2+. In total, about 40% of the residues are identical, including the site of the proposed cis-trans isomerization underlying the locked to unlocked conformational change. It will be of interest to compare the kinetics of binding of metals to favin with the results from Con A. 

A series of small peptides were prepared in order to identify the minimal unit that could mimic the effect of the N-terminal cap domain. The structure of the most efficient (I19L, Fig. 35) was studied by NMR and molecular modeling [142]. The concentration dependence of inhibition of tubulin polymerization suggested that the peptides bind stoichiometrically to unpolymerized tubulin rather than to assembled MT. It is well known that the stability of the tubulin/stathmin complex decreases upon phosphorylation of stathmin [8]. In agreement with this, phosphorylated I19L was found to be fourfold less efficient than its unphosphorylated form. 

Equations (45.13) and (45.14) assume that the mAb binds stoichiometrically with the target receptor in a 1 1 ratio, and Eq. (45.14) assumes that the total number of target receptors remains constant. Since the binding to target receptors was assumed to be in equilibrium at all times, binding was also taken into account when mAb is transferred from the dose compartment to the central compartment, with the central compartment only accounting for free mAb that is in equilibrium with bound mAb. Although not immediately apparent, these model structures preserve the overall mass balance in the model. 

Floor, M., and Biobel, G., (1996). The nuclear transport factor karyopherin /3 binds stoichiometrically to Ran-GTP and inhibits the RAN GTPase activating protein, i. Biol. Chem. 271, 5313-5316. 

D binds stoichiometrically to the SO3 groups of polystyrene sulfonic acid (PSS). The binding process was followed by titrating D with PSS in aqueous solution a change on the absorption spectrum was observed and was attributed to the formation of intermolecular associated species among dye molecules crowded along the polyanion chain. 

Several investigators (Sharma and Edwards 1983 Zelazowski et al. 1984 Kraker et al. 1985) reported that cisplatin reacts with metallothionein in vitro. Cisplatin binds stoichiometrically to the protein sulfhydryl groups in metallothionein and Zn, if present, is completely replaced with 10 2g atoms Pt/mol protein (Pattanaik et al. 1992). The amine ligands in cisplatin are also lost, suggesting that Pt(II) has tetrathiolate coordination in metal-... 

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