HaCaT cell lines

Small water-soluble molecules can also be introduced into cells via stimulation of fluid-phase uptake by the described protocol. Uptake of Lucifer Yellow (m.w. 457.2 Lucifer Yellow CH, dilithium salt from Sigma Chemicals, Rehovot, Israel) has been stimulated by 2.2-fold and 2.7-fold in COS 5-7 and HaCaT cell lines, respectively, as compared with the constitutive uptake. 

Mariani et al. compared the effects of light fixation in 2% formalin, air drying and desiccation for 1 h each on two different cell lines using Raman spectroscopy. For human keratinocyte cells (HaCaT cell line), air dried... 

Figure 5.6 Principal components analysis (PCA) score plot for Raman spectra of (A) A549, (B) BEAS2B and (C) HaCaT cell lines. Percentage labels on each axis denote the variance described by that PC. There is clear separation of cellular spectra fixed with Meth-Ac and Carnoy s fixative relative to live and formalin fixed spectra. A degree of similarity between the spectral content of formalin fixed and live cell spectra is implied by the proximity of their clusters. (Reproduced from reference [52].)...

The tamarind seed XG exerts several biological activities, such as marked inhibitory effect on the binding of BK virus to cells [299] and immunomodulatory effects [300]. Recently, it was reported [301] that XG affects the proliferation of cytokines in various skin-cell lines (Fig. 14) such as HaCaT cells (im-... 

Fig. 14 Effect of the molecular weight of tamarind seed xyloglucan depolymerized by ( ) 7-irradiation, ( ) ultrasonication, and ( ) endo-glucanase treatment on the production of various cytokines (Tumor necrosis factor a, TNE-a Interleukin 8, IL-8 Interleukin 10, IL-10 and Interleukin 12, IL-12) in HaCaT cells (Immortalized keratinocytes line) [301]...

Doxycycline inhibits the detachment of SM-exposed HaCaT cells in culture from the growth substrate. However, analysis of the metabolic activity of the adherent cells reveals that doxycycline treatment does not maintain cell viability. It is suggested that doxycycline and other matrix metalloprotease inhibitors may have a role to play in therapeutic intervention against SM, only as a combination therapy (Lindsay et al, 2007). Human keratinocyte cell lines were pretreated with mixtures of methenamine and glutathione prior to SM exposure. Though it is possible to protect the cell cultures from the toxic effects of SM, it will be effective only as a pretreatment (Smith et al, 1997). 

Figure 20.16 Fluorescence spectra of HSC 3 cells incubated with nanosphere solution. = 280 nm. Overall, gold nanospheres decrease the fluorescence of cells however, in some samples an increase in fluorescence is observed. The data is similar for all three cell lines (HaCat, HOC 313, and HSC 3) when the nanopaiticles were incubated with cells. Cited from reference 52.

In the naphthoquinone group, a number of lapacho compounds, the most common naphthoquinonic constituents of the inner bark of Tabebuia impetiginosa (Mart ex D C.) Standi were evaluated in vitro against the growth of the human keratinocyte cell line HaCaT. All compounds inhibited keratinocyte growth and appear to be promising as effective antipsoriatic agent [147],... 

Reeently, Saliou and coworkers have reported that oral supplementation of Pyenogenol reduees UV-induced erythema in the hitman skin and also significant inhibits the aetivatiorr, the binding to DNA, and the NF-rcB-dependent gene expression in the immortalized human keratinoeyte cell line HaCaT. In the same eontext, Pycnogenol has been observed to proteet GSH levels and to partially suppress the cytotoxicity induced by UV treatment, both in human primary keratinoeytes and in HaCaT cells. ... 

On the other hand, replacement of the morpholino moiety (ring C) by a 3-methyl-l,2,4-oxadiazol-5-yl g oup produces active compounds (2013EJMC533). Compounds 78a—d (F yne 27) show lower activity compared to linezolid against standard S. aureus, but a direct comparison could not be performed because the test were performed on racemic mixtures. Nevertheless, as a racemate, thioamide derivatives 78c,d showed major activity against MRSA strains, with MIC values twofold lower than those of reference drug. Moreover, a safety profile similar to HnezoHd was evidenced by means of cytotoxicity data on PK15, HaCat, and HepG2 cell lines. 

Pinus densifiora Sieb. et Zucc. (Pinaceae) EO inhibited proliferation and induced apoptosis in YD 8 cells (human oral squamous carcinoma). Cell proliferation was inhibited by 30% and 60% at concentrations of 40 and 60 pg/mL EO. The EO reduced cancer cells by 70% when treated with 60 pg/mL EO. Apoptosis was induced by activation of caspase-9 (Jo et al., 2012). Soeur et al. (2011) found ihdXAniba rosaeodora Ducke (Lauraceae) had cytotoxic effects against A431 (human epidermoid carcinoma cell line) and on HaCaT (human keratinocytes) cells. At a concentration... 

HaCaT (human adult low calcium high temperature) cells are spontaneously transformed human keratinocytes which have characteristics of basal epidermal keratinocytes so that cell line may be used as an in vitro model for highly proliferative epidermis in tissue engineering [4]. 

Human immortalised non-tumorigenic keratinocyte HaCaT cells (CeU Lines Service, Germany) were seeded mito the treated and untreated samples in the culture dishes and incubated at 37 °C for 4 days [10]. DMEM (high glucose) supplemented with 2mM L-glutamine solution and 10% fetal calf serum was used as the culmre medium. 

Usnic acid Methyl a-orcinolcarboxylate Ethyl hematommate Diffractaic acid (+) Protolichesterinic acid Human keratinocyte cell line HaCaT Kumar and Muller (1999)... 

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