HaCaT keratinocytes

Geiges D, Marks F, Gschwendt M (1995) Loss of protein kinase C5 from human HaCaT keratinocytes upon ras transfection is mediated by TGFa. Exp Cell Res 219 299-303... 

Masaki H, Okano Y Ochiai Y et al. Alpha-tocopherol increases the intracellular glutathione levels in HaCaT keratinocytes. Free Rad Res 2002 36 705-709. 

Yin JJ, Liu J, Ehrenshaft M, Roberts JE, Fu PP, Mason RP, Zhao B (2012) Phototoxicity of nano titanium dioxides in HaCaT keratinocytes—generation of reactive oxygen species and cell damage. Toxicol Appl Pharmacol 263(l) 81-88... 

The EPDIM sequence is derived from an ECM protein, pig-h3. Bae et al. [50] reported that EPDIM promoted keratinocyte adhesion, proliferation, and migration by integrin-mediated interactions. Compared to those unmodified materials, the EPDIM-immobUized chitosan porous beads significantly enhanced the growth rate of NIH3T3 fibroblasts and HaCaT keratinocytes, which is promising for the wound healing of injured tissue such as skin. 

Afaq, F. et al., Delphinidin, an anthocyanidin in pigmented fruits and vegetables, protects human HaCaT keratinocytes and mouse skin against UVB-mediated oxidative stress and apoptosis, J. Invest. Dermatol.,... 

HaCaT keratinocyte cells exposed to the compounds juglone or plumbagin at concentrations of 0 to 20 pM exhibited dose-dependent decrease in cell viability. The cytotoxicity of these compounds is due to two different mechanisms, namely, redox cycling and reaction with glutathione (Inbaraj and Chignell 2004). 

Haertel, B., et al. Non-thermal atmospheric-pressure plasma can influence cell adhesion molecules on HaCaT-keratinocytes. Experimental Dermatology 20(3), 282-284 (2011)... 

Zhou, B.-R, Zhang, J.-A., Zhang, Q., Permatasari, F., Xu, Y., Wu, D., Luo, D. (2013). Palmitic Acid Induces Production of Proinflammatory Cytokines Interleukin-6, Interleukin-1, and Tumor Necrosis Factor- via a NF-B-Dependent Mechanism in HaCaT Keratinocytes.MediatorsInflamm, 2013, ll.doi 10.1155/2013/530429. 

Inbaraj, J. J. Kukielczak, B. M. Chigneh, C. F. Phloxine B phototoxicity a mechanistic study using HaCaT keratinocytes. Photochem. Photobiol. 2005, 81, 81-88. 

Boissel, J.-R, Ohly, D., Bros, M., Godtel-Armbrust, U., Forstermann, U., Frank, S., 2003. The neuronal nitric oxide synthase is upregulated in mouse skin repair and in response to epidermal growth factor in human HaCaT keratinocytes. Journal of Investigative Dermatology 123, 132-139. 

Fig. 5 Micrographs of human skin HaCaT keratinocytes in culture upon the collagen films. (A) untreated, (B) air and (C) nitrogen treated...

More recent studies have suggested that MMP-9 is released by fibroblasts in response to keratinocyte stimulation by SM (Ries et al., 2008). Mustard-stimulated release of MMP-9 only occurred in coculture of human dermal fibroblasts wi HaCaT keratinocytes or with culture of human dermal fibroblasts with conditioned media from HD-treated HaCaT cells, suggesting that paracrine signaling is responsible (Ries et al., 2008). Our work showed a dramatic increase in cytokine expression and activation of mediators of inflammation within 24 h after HD exposure, which may contribute to MMP-9 induction (Figure 39.6) (Gerecke et al., 2009). Together these data suggest that inhibition of fibroblast activation after vesicant exposure may also reduce MMP-9 activation and release. 

Ermolli, M. Menne, C. Pozzi, G. Serra, M. A. Clerici, L. A. Nickel, cobalt and chromium-induced cytotoxicity and intracellular accumulation in human hacat keratinocytes. Toxicology 2001, 159, 23-31. 

Wang, S. Sheng, Y. Feng, M. Leszczynski, J. Wang, L. Tachikawa, H. Yu, H. 2007. Light-Induced Cytotoxicity of 16 Polycyclic Aromatic Hydrocarbons on the US EPA Priority Pollutant List in Human Skin HaCaT Keratinocytes Relationship Between Phototoxicity and Excited State Properties. Environmental Toxicology, v.22, p.318-327. 

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